The effects of recombinantly derived growth hormone (GH), oestradiol-17β plus trenbolone acetate, clofibrate, melatonin and fasting on basal plasma triglycerides (TG) and TG entry rate were determined in wether lambs given lucerene/barley pellets ad libitum. Clofibrate was administered at 2 × 0·5 g capsules orally, GH (0·25 mg/kg live weight) was injected subcutaneously daily whilst oestradiol-17β, oestradiol-17β plus trenbolone acetate and melatonin were slow release implants (either 4 or 4·8 mg, 4 nig and 20 nig and 18 mg respectively) placed subcutaneously in the ear. Both fasting and oestradiol treatments raised basal TG levels without influencing TG entry rates, defined as the maximum rate of change of plasma TG 3 to 6h following a dose of Triton WR1339 of at least 0·2 g/kg live weight. Entry rates were similar to those previously reported for goats but were higher than those reported for sheep. Triton WR1339 used to determine entry rates was found to reduce food intake at all levels in some sheep despite reports to the contrary. Among the various treatments, only oestradiol-17β and trenbolone acetate influenced body composition as determined by chemical analysis of minced carcasses, resulting in an increased gain of carcass protein and decreased gain of carcass fat. Clofibrate, melatonin and Triton, if allowance is made for Triton-induced loss in food intake, had no clear effects on body fatness. Overall the effects of hormones on plasma TG appeared to be small and it seems improbable that plasma TG are involved in effects of hormones on fatness.