Although web-based data collection has become increasingly popular in (linguistic) research over the past years, many researchers are still cautious about collecting data via the internet. Thus, this study aims at comparing web-based and lab-based testing of linguistic manipulations that have resulted in robust findings in previous lab-based research on bilingual language processing. A total of 134 L1 German students of L2 English participated in two experiments in a web-based (n = 78) or lab-based setting (n = 56). The study examined potential language co-activation through cognates in an English Lexical Decision Task (Experiment 1) and the use of L2 lexical and syntactic information in English relative clause processing in a Self-paced Reading Task (Experiment 2). We found comparable evidence of lexical and syntactic processing in both groups in both experiments. Critically, this paper provides important methodological implications for web-based data collections with second language learners.