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  3. Advances in Microscopy and Microanalysis

Microscopic visualization techniques range from atomic imaging to visualization of living cells at near nanometer spatial resolution, and advances in the field are fueled by developments in computation, image detection devices, labelling, and sample preparation strategies. Microscopy has proven to be one of the most attractive and progressive research tools available to the scientific community, and remains at the forefront of research in many disciplines, from nanotechnology to live cell molecular imaging. This series reflects the diverse role of microscopy, defining it as any method of imaging objects of micrometer scale or less, and includes both introductory texts and highly technical and focused monographs for researchers and practitioners in materials and the life sciences.

  • General Editors: Patricia Calarco, University of California, San Francisco, Michael Isaacson, University of California, Santa Cruz

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5 results in Advances in Microscopy and Microanalysis

Atomic-Scale Analytical Tomography
  • Concepts and Implications
  • Thomas F. Kelly, Brian P. Gorman, Simon P. Ringer
  • Published online:
    03 March 2022
    Print publication:
    24 March 2022
  • A comprehensive guide on Atomic-Scale Analytical Tomography (ASAT) that discusses basic concepts and implications of the technique in areas such as material sciences, microscopy, engineering sciences and several interdisciplinary avenues. The title interrogates how to successfully achieve ASAT at the intersection of transmission electron microscopy and atom probe microscopy. This novel concept is capable of identifying individual atoms in large volumes as well as in 3D, with high spatial resolution. Written by leading experts from academia and industry, this book serves as a guide with real-world applications on cutting-edge research problems. An essential reading for researchers, engineers and practitioners interested in nanoscale characterisation, this book introduces the reader to a new direction for atomic-scale microscopy.

X-ray Microscopy
  • Chris Jacobsen
  • Published online:
    28 October 2019
    Print publication:
    19 December 2019
  • Written by a pioneer in the field, this text provides a complete introduction to X-ray microscopy, providing all of the technical background required to use, understand and even develop X-ray microscopes. Starting from the basics of X-ray physics and focusing optics, it goes on to cover imaging theory, tomography, chemical and elemental analysis, lensless imaging, computational methods, instrumentation, radiation damage, and cryomicroscopy, and includes a survey of recent scientific applications. Designed as a 'one-stop' text, it provides a unified notation, and shows how computational methods in different areas are linked with one another. Including numerous derivations, and illustrated with dozens of examples throughout, this is an essential text for academics and practitioners across engineering, the physical sciences and the life sciences who use X-ray microscopy to analyze their specimens, as well as those taking courses in X-ray microscopy.

Wavefront Shaping for Biomedical Imaging
  • Edited by Joel Kubby, Sylvain Gigan, Meng Cui
  • Published online:
    10 June 2019
    Print publication:
    20 June 2019
  • Learn about the theory, techniques and applications of wavefront shaping in biomedical imaging using this unique text. With authoritative contributions from researchers who are defining the field, cutting-edge theory is combined with real-world practical examples, experimental data and the latest research trends to provide the first book-level treatment of the subject. It is suitable for both background reading and use in a course, with coverage of essential topics such as adaptive optical microscopy, deep tissue microscopy, time reversal and optical phase conjugation, and tomography. The latest images from the forefront of biomedical imaging are included, and full-colour versions are available in the eBook version. Researchers, practitioners and graduate students in optics, biophotonics, biomedical engineering, and biology who use biomedical imaging tools and are looking to advance their knowledge of the subject will find this an indispensable resource.

Liquid Cell Electron Microscopy
  • Edited by Frances M. Ross
  • Published online:
    22 December 2016
    Print publication:
    24 November 2016
  • The first book on the topic, with each chapter written by pioneers in the field, this essential resource details the fundamental theory, applications, and future developments of liquid cell electron microscopy. This book describes the techniques that have been developed to image liquids in both transmission and scanning electron microscopes, including general strategies for examining liquids, closed and open cell electron microscopy, experimental design, resolution, and electron beam effects. A wealth of practical guidance is provided, and applications are described in areas such as electrochemistry, corrosion and batteries, nanocrystal growth, biomineralization, biomaterials and biological processes, beam-induced processing, and fluid physics. The book also looks ahead to the future development of the technique, discussing technical advances that will enable higher resolution, analytical microscopy, and even holography of liquid samples. This is essential reading for researchers and practitioners alike.

Scanning Electron Microscopy for the Life Sciences
  • Edited by Heide Schatten
  • Published online:
    05 January 2013
    Print publication:
    06 December 2012
  • Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM and highlights several advances in cell and molecular biology that greatly benefited from using conventional, cryo, immuno and high-resolution SEM.