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Chapter 2 - Antigens

Published online by Cambridge University Press:  30 January 2025

By
Marie-Christine Béné  and
Anna Porwit
Edited by
Anna Porwit  and
Marie Christine Béné
Anna Porwit
Affiliation:
Lunds Universitet, Sweden
Marie Christine Béné
Affiliation:
Université de Nantes, France
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Summary

Flow cytometry relies on the use of fluorochrome-conjugated antibodies, most of them identified and produced after the discovery of the technology allowing to generate large amounts of monoclonal antibodies. Hence, nearly all these reagents are named after the cluster of differentiation (CD) number that was given to newly discovered molecules they recognize, many of them having no other name. Although some CDs have become very popular and well known, others are less familiar. This chapter provides a guide to recover the characteristics of surface or cytoplasmic antigens explored with the CDs most frequently used in the field of haematological malignancies.

Keywords

flow cytometrymonoclonal antibodiesantigenscluster of differentiationubiquitous antigenslineage-specific antigensmolecule structuremolecule functionssurface markerscytoplasmic markers
Type
Chapter
Information
Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies , pp. 15 - 35
Publisher: Cambridge University Press
Print publication year: 2025

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