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Chapter 2 - Development of ICSI in Human Assisted Reproduction

Published online by Cambridge University Press:  02 December 2021

By
Stephanie Cheung ,
Alessandra Parrella ,
Philip Xie ,
Sherina Lawrence ,
Mounia Haddad ,
Derek Keating ,
Zev Rosenwaks  and
Gianpiero D. Palermo
Edited by
Gianpiero D. Palermo  and
Zsolt Peter Nagy
Gianpiero D. Palermo
Affiliation:
Cornell Institute of Reproductive Medicine, New York
Zsolt Peter Nagy
Affiliation:
Reproductive Biology Associates, Atlanta, GA
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Summary

Since the establishment of in vitro fertilization, it became quickly apparent that approximately half of the couples treated presented with a dysfunctional male gamete. To alleviate this issue, intracytoplasmic sperm injection (ICSI) was introduced to treat men with compromised semen parameters or azoospermia, and more recently high sperm chromatin fragmentation or sperm-linked oocyte activation deficiency. Because of its success, ICSI has been extended for cases with low egg yield, oocyte cryopreservation, and often for preimplantation genetic testing. Due to its versatility and reliability, ICSI has become the most popular ART and will be invaluable for emerging technologies such as in vitro gametogenesis and heritable genome editing. In this chapter, we discuss the development of ICSI, its current applications, and ongoing research that will contribute to the future of reproductive medicine.

Keywords

intracytoplasmic sperm injectionpreimplantation genetic testingsurgical sperm retrievalDNA fragmentationassisted oocyte activationoocyte dysmorphismoocyte maturityoocyte cryopreservationgametogenesisheritable genome editing
Type
Chapter
Information
Manual of Intracytoplasmic Sperm Injection in Human Assisted Reproduction
With Other Advanced Micromanipulation Techniques to Edit the Genetic and Cytoplasmic Content of the Oocyte
 , pp. 11 - 24
Publisher: Cambridge University Press
Print publication year: 2021

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