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Chapter 10 - Flow Cytometric Diagnosis of Classic Hodgkin Lymphoma in Lymph Nodes

Published online by Cambridge University Press:  30 January 2025

By
Lorinda Soma  and
Jonathan R. Fromm
Edited by
Anna Porwit  and
Marie Christine Béné
Anna Porwit
Affiliation:
Lunds Universitet, Sweden
Marie Christine Béné
Affiliation:
Université de Nantes, France
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Summary

Hodgkin lymphoma, a nodal disease, is usually diagnosed using morphology and immunochemistry on lymph nodes biopsies. However, with the increased practice of fine-needle aspiration or core biopsy, multiparameter flow cytometry (MFC) can provide valuation information on cell suspensions from such samples. Here, the major markers and characteristics allowing, in MFC, to distinguish between the scarce Reed Sternberg cells and the inflammatory immunological infiltrate surrounding them are described. Guidelines and recent information are provided for readers willing to implement these investigations in their own settings.

Keywords

flow cytometryimmunophenotypingclassic Hodgkin lymphomanodular lymphocyte predominant Hodgkin lymphomafine needle aspirationcore biopsycell suspensionspanelsCD30gating strategies
Type
Chapter
Information
Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies , pp. 164 - 173
Publisher: Cambridge University Press
Print publication year: 2025

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References

Küppers, R, Engert, A, Hansmann, ML. Hodgkin lymphoma. J Clin Invest 2012; 122: 3439–3447.CrossRefGoogle ScholarPubMed
Alaggio, R, Amador, C, Anagnostopoulos, I, et al. The 5th edition of the World Health Organization Classification of Haematolymphoid Tumours: Lymphoid Neoplasms. Leukemia 2022; 36: 1720–1748.CrossRefGoogle ScholarPubMed
Fromm, JR, Kussick, SJ, Wood, BL. Identification and purification of classical Hodgkin cells from lymph nodes by flow cytometry and flow cytometric cell sorting. Am J Clin Pathol 2006; 126: 764–780.CrossRefGoogle ScholarPubMed
Fromm, JR, Sabath, DE, Wood, BL. Diagnostic usefulness of flow cytometry for immunophenotyping classical Hodgkin lymphoma. Am J Clin Pathol 2011; 136: 157–158.CrossRefGoogle ScholarPubMed
Fromm, JR, Wood, BL. A six-color flow cytometry assay for immunophenotyping classical Hodgkin lymphoma in lymph nodes. Am J Clin Pathol 2014; 141: 388–396.CrossRefGoogle ScholarPubMed
Irsch, J, Nitsch, S, Hansmann, ML, et al. Isolation of viable Hodgkin and Reed-Sternberg cells from Hodgkin disease tissues. Proc Natl Acad Sci USA 1998; 95: 10117–10122.CrossRefGoogle ScholarPubMed
Kim, LH, Eow, GI, Peh, SC, Poppema, S. The role of CD30, CD40 and CD95 in the regulation of proliferation and apoptosis in classical Hodgkin’s lymphoma. Pathology 2003; 35: 428–435.CrossRefGoogle ScholarPubMed
Tedoldi, S, Mottok, A, Ying, J, et al. Selective loss of B-cell phenotype in lymphocyte predominant Hodgkin lymphoma. J Pathol 2007; 213: 429–440.CrossRefGoogle ScholarPubMed
Ally, F, Gajzer, D, Fromm, JR. A review of the flow cytometric findings in classic Hodgkin lymphoma, nodular lymphocyte predominant Hodgkin lymphoma and T cell/histiocyte-rich large B cell lymphoma. Clin Lab Med 2023; 43: 427–444.CrossRefGoogle ScholarPubMed
Banchereau, J, Bazan, F, Blanchard, D, et al. The CD40 antigen and its ligand. Annu Rev Immunol 1994; 12: 881–922.CrossRefGoogle ScholarPubMed
Dorreen, MS, Habeshaw, JA, Stansfeld, AG, Wrigley, PF, Lister, TA. Characteristics of Sternberg-Reed, and related cells in Hodgkin’s disease: an immunohistological study. Br J Cancer 1984; 49: 465–476.CrossRefGoogle ScholarPubMed
Peter, ME, Budd, RC, Desbarats, J, et al. The CD95 receptor: apoptosis revisited. Cell 2007; 129: 447–450.CrossRefGoogle ScholarPubMed
Fromm, JR, Wood, BL. Strategies for immunophenotyping and purifying classical Hodgkin lymphoma cells from lymph nodes by flow cytometry and flow cytometric cell sorting. Methods 2012; 57: 368–375.CrossRefGoogle ScholarPubMed
Chan, A, Scarpa Carniello, JV, Gao, Q, et al. Role of flow cytometric immunophenotyping for classic Hodgkin lymphoma in small biopsy and cytology specimens. Arch Pathol Lab Med 2022; 146: 462–468.CrossRefGoogle ScholarPubMed
Carbone, A, Gloghini, A, Gattei, V, et al. Expression of functional CD40 antigen on Reed-Sternberg cells and Hodgkin’s disease cell lines. Blood 19951; 85: 780–789.CrossRefGoogle ScholarPubMed
Kesler, MV, Paranjape, GS, Asplund, SL, McKenna, RW, Jamal, S, Kroft, SH. Anaplastic large cell lymphoma: a flow cytometric analysis of 29 cases. Am J Clin Pathol 2007; 128: 314–322.CrossRefGoogle ScholarPubMed
Segal, GH, Kjeldsberg, CR, Smith, GP, Perkins, SL. CD30 antigen expression in florid immunoblastic proliferations: a clinicopathologic study of 14 cases. Am J Clin Pathol 1994; 102: 292–298.CrossRefGoogle ScholarPubMed
Barry, TS, Jaffe, ES, Sorbara, L, Raffeld, M, Pittaluga, S. Peripheral T-cell lymphomas expressing CD30 and CD15. Am J Surg Pathol 2003; 27: 1513–1522.CrossRefGoogle ScholarPubMed
Kanzler, H, Küppers, R, Helmes, S, et al. Hodgkin and Reed-Sternberg-like cells in B-cell chronic lymphocytic leukemia represent the outgrowth of single germinal-center B-cell-derived clones: potential precursors of Hodgkin and Reed-Sternberg cells in Hodgkin’s disease. Blood 2000; 95: 1023–1031.CrossRefGoogle ScholarPubMed
Hernandez, O, Oweity, T, Ibrahim, S. Is an increase in CD4/CD8 T-cell ratio in lymph node fine needle aspiration helpful for diagnosing Hodgkin lymphoma? A study of 85 lymph node FNAs with increased CD4/CD8 ratio. Cytojournal 2005; 2: 14.CrossRefGoogle Scholar
Bosler, DS, Douglas-Nikitin, VK, Harris, VN, Smith, MD. Detection of T-regulatory cells has a potential role in the diagnosis of classical Hodgkin lymphoma. Cytometry B Clin Cytom 2008; 74: 227–235.CrossRefGoogle Scholar
Fromm, JR, Thomas, A, Wood, BL. Increased expression of T cell antigens on T cells in classical Hodgkin lymphoma. Cytometry B Clin Cytom 2010; 78: 387–388.CrossRefGoogle Scholar
Seegmiller, AC, Karandikar, NJ, Kroft, SH, McKenna, RW, Xu, Y. Overexpression of CD7 in classical Hodgkin lymphoma-infiltrating T lymphocytes. Cytometry B Clin Cytom 2009; 76: 169–174.CrossRefGoogle ScholarPubMed
Wu, D, Thomas, A, Fromm, JR. Reactive T cells by flow cytometry distinguish Hodgkin lymphomas from T cell/histiocyte-rich large B cell lymphoma. Cytometry B Clin Cytom 2016; 90: 424–432.CrossRefGoogle ScholarPubMed
Beatriz Álvarez Flores, M, Sopeña Corvinos, M, Medrano Élez, M, et al. A new approach to the study of Hodgkin lymphoma by flow cytometry. Pathology 2023; 55: 86–93.CrossRefGoogle Scholar
Fromm, JR, Thomas, A, Wood, BL. Characterization and purification of neoplastic cells of nodular lymphocyte predominant Hodgkin lymphoma from lymph nodes by flow cytometry and flow cytometric cell sorting. Am J Pathol 2017; 187: 304–317.CrossRefGoogle ScholarPubMed
Grewal, RK, Chetty, M, Abayomi, EA, Tomuleasa, C, Fromm, JR. Use of flow cytometry in the phenotypic diagnosis of Hodgkin’s lymphoma. Cytometry B Clin Cytom 2019; 96: 116–127.CrossRefGoogle ScholarPubMed
Martig, DS, Fromm, JR. A comparison and review of the flow cytometric findings in classic Hodgkin lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, T cell/histiocyte rich large B cell lymphoma, and primary mediastinal large B cell lymphoma. Cytometry B Clin Cytom 2022; 102: 14–25.CrossRefGoogle Scholar
Steidl, C, Lee, T, Shah, SP, et al. Tumor-associated macrophages and survival in classic Hodgkin’s lymphoma. N Engl J Med 2010; 362: 875–885.CrossRefGoogle ScholarPubMed
Portlock, CS, Donnelly, GB, Qin, J, et al. Adverse prognostic significance of CD20 positive Reed-Sternberg cells in classical Hodgkin’s disease. Br J Haematol 2004; 125: 701–708.CrossRefGoogle ScholarPubMed
Oudejans, JJ, Jiwa, NM, Kummer, JA, et al. Activated cytotoxic T cells as prognostic marker in Hodgkin’s disease. Blood 1997; 89: 1376–1382.CrossRefGoogle ScholarPubMed
Sup, SJ, Alemañy, CA, Pohlman, B, et al. Expression of bcl-2 in classical Hodgkin’s lymphoma: an independent predictor of poor outcome. J Clin Oncol 2005; 23: 3773–3779.CrossRefGoogle ScholarPubMed
Chetaille, B, Bertucci, F, Finetti, P, et al. Molecular profiling of classical Hodgkin lymphoma tissues uncovers variations in the tumor microenvironment and correlations with EBV infection and outcome. Blood 2009; 113: 2765–2775.CrossRefGoogle ScholarPubMed
Fromm, JR, Tang, C, Naresh, KN. Predictors of risk of relapse in classic Hodgkin lymphoma. J Clin Pathol 2023; 76: 414–417.CrossRefGoogle ScholarPubMed
Armand, P, Shipp, MA, Ribrag, V, et al. Programmed death-1 blockade with pembrolizumab in patients with classical Hodgkin lymphoma after brentuximab vedotin failure. J Clin Oncol 2016; 34: 3733–3739.CrossRefGoogle ScholarPubMed
Tzankov, A, Zimpfer, A, Lugli, A, et al. High-throughput tissue microarray analysis of G1-cyclin alterations in classical Hodgkin’s lymphoma indicates overexpression of cyclin E1. J Pathol 2003; 199: 201–207.CrossRefGoogle ScholarPubMed
Fromm, JR. Flow cytometric analysis of CD123 is useful for immunophenotyping classical Hodgkin lymphoma. Cytometry B Clin Cytom 2011; 80: 91–99.CrossRefGoogle ScholarPubMed
Simonson, PD, Wu, Y, Wu, D, Fromm, JR, Lee, AY. De novo identification and visualization of important cell populations for classic Hodgkin lymphoma using flow cytometry and machine learning. Am J Clin Pathol 2021; 156: 1092–1102.CrossRefGoogle ScholarPubMed

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