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Chapter 10 - Flow Cytometric Diagnosis of Classic Hodgkin Lymphoma in Lymph Nodes

Published online by Cambridge University Press:  30 January 2025

By
Lorinda Soma  and
Jonathan R. Fromm
Edited by
Anna Porwit  and
Marie Christine Béné
Anna Porwit
Affiliation:
Lunds Universitet, Sweden
Marie Christine Béné
Affiliation:
Université de Nantes, France
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Summary

Hodgkin lymphoma, a nodal disease, is usually diagnosed using morphology and immunochemistry on lymph nodes biopsies. However, with the increased practice of fine-needle aspiration or core biopsy, multiparameter flow cytometry (MFC) can provide valuation information on cell suspensions from such samples. Here, the major markers and characteristics allowing, in MFC, to distinguish between the scarce Reed Sternberg cells and the inflammatory immunological infiltrate surrounding them are described. Guidelines and recent information are provided for readers willing to implement these investigations in their own settings.

Keywords

flow cytometryimmunophenotypingclassic Hodgkin lymphomanodular lymphocyte predominant Hodgkin lymphomafine needle aspirationcore biopsycell suspensionspanelsCD30gating strategies
Type
Chapter
Information
Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies , pp. 164 - 173
Publisher: Cambridge University Press
Print publication year: 2025

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