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Chapter 9 - Mature T-Cell Neoplasms and Natural Killer-Cell Malignancies

Published online by Cambridge University Press:  30 January 2025

By
Anne Tierens
Edited by
Anna Porwit  and
Marie Christine Béné
Anna Porwit
Affiliation:
Lunds Universitet, Sweden
Marie Christine Béné
Affiliation:
Université de Nantes, France
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Summary

Mature T- and natural killer (NK)-cell neoplasms comprise multiple distinct disease entities. Diagnosis and classification of these entities require the integration of morphology, immunophenotype and cyto- and molecular genetics and correlation with clinical presentation. Multiparameter flow cytometry (MFC) is an important tool to immunophenotype T and NK cells. Our knowledge of the constellation of immunophenotypic aberrancies associated with certain disease entities has increased by the simultaneous analysis of more markers and molecular genetic studies. Genotype-phenotype associations have been identified contributing to a better understanding of the disease biology and clinical behaviour. T- and NK-cell disease entities in which MFC plays a central role in the diagnosis and classification are reviewed in this chapter. T-cell clonality analysis by MFC has become an assay used in many diagnostic laboratories. The availability of the JOVI-1 antibody against the T-cell receptor β constant region 1 protein (TRBC1) has greatly facilitated the detection of clonal TCRαβ T cells with high specificity and sensitivity. Despite the major advances in the diagnostic flow cytometry assays for the detection of T- and NK-cell neoplasms, standardized protocols are needed to increase the accuracy of diagnosis and classification and facilitate the implementation of automated MFC data analysis.

Keywords

flow cytometryimmunophenotypingT-cell lymphomaNK-cell lymphomasubtypesclassificationpanelsT-cell receptorTRBCmonotypy
Type
Chapter
Information
Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies , pp. 141 - 163
Publisher: Cambridge University Press
Print publication year: 2025

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