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Chapter 11 - Preparation of Surgically Retrieved Spermatozoa

Published online by Cambridge University Press:  16 February 2022

David Mortimer ,
Lars Björndahl ,
Christopher L. R. Barratt ,
José Antonio Castilla ,
Roelof Menkveld ,
Ulrik Kvist ,
Juan G. Alvarez  and
Trine B. Haugen
David Mortimer
Affiliation:
Oozoa Biomedical Inc., Vancouver
Lars Björndahl
Affiliation:
Karolinska Institutet, Stockholm
Christopher L. R. Barratt
Affiliation:
University of Dundee
José Antonio Castilla
Affiliation:
HU Virgen de las Nieves, Granada
Roelof Menkveld
Affiliation:
Stellenbosch University, South Africa
Ulrik Kvist
Affiliation:
Karolinska Institutet, Stockholm
Juan G. Alvarez
Affiliation:
Centro ANDROGEN, La Coruña
Trine B. Haugen
Affiliation:
Oslo Metropolitan University
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Summary

Standard operating procedures (SOPs) for processing both epididymal and testicular spermatozoa are provided.

Keywords

surgical sperm retrievalepididymistestisMESATESE
Type
Chapter
Information
A Practical Guide to Basic Laboratory Andrology , pp. 256 - 261
Publisher: Cambridge University Press
Print publication year: 2022

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References

Shin, DH, Turek, PJ. Sperm retrieval techniques. Nat Rev Urol 2013; 10: 723–30.Google Scholar
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Verheyen, G, Popovic-Todorovic, B, Tournaye, H. Processing and selection of surgically retrieved sperm for ICSI: a review. Basic Clin Androl 2017; 27: 6.CrossRefGoogle ScholarPubMed
Flannigan, RK, Schlegel, PN. Microdissection testicular sperm extraction: preoperative patient optimization, surgical technique, and tissue processing. Fertil Steril 2019; 111: 420–6.Google Scholar
Rowe, PJ, Comhaire, FH, Hargreave, TB, Mahmoud, AMA. WHO Manual for the Standardized Investigation, Diagnosis and Management of the Infertile Male. Cambridge: Cambridge University Press, 2000.Google Scholar
Deruyver, Y, Vanderschueren, D, Van der Aa F. Outcome of microdissection TESE compared with conventional TESE in non-obstructive azoospermia: a systematic review. Andrology 2014; 2: 20–4.Google Scholar
Bernie, AM, Mata, DA, Ramasamy, R, Schlegel, PN. Comparison of microdissection testicular sperm extraction, conventional testicular sperm extraction, and testicular sperm aspiration for nonobstructive azoospermia: a systematic review and meta-analysis. Fertil Steril 2015; 104: 1099–103.CrossRefGoogle ScholarPubMed

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