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Chapter 16 - Cryopreservation of Sperm for IVF

Semen Samples and Individual Sperm

from Section 3 - Gametes

Published online by Cambridge University Press:  07 August 2023

By
Nina Desai  and
Pooja Rambhia
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Cryopreservation of human spermatozoa offers male patients the option to preserve fertility prior to initiating cancer therapy, vasectomy or assisted reproductive technology (ART) treatment. Human sperm can be easily cryopreserved in liquid nitrogen and stored for years. A number of cryopreservation techniques and cryoprotectants have been investigated. To date, however, vapour-phase or programmed slow cooling using a glycerol/egg yolk buffer cryoprotectant has been the most widely applied sperm cryopreservation protocol. Sperm cryosurvival rates of 40% to 60% are generally achievable. However, in patients having low motility or low sperm number at the outset, cryopreservation and recovery of viable sperm can be more challenging. In such cases, freezing of single sperm or small numbers of sperm is emerging as a valuable tool. With this technique, intracytoplasmic sperm injection (ICSI) can be performed even in cases with only a few frozen spermatozoa.

Keywords

cryopreservationstoragesemensperm
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 121 - 129
Publisher: Cambridge University Press
Print publication year: 2023

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References

Polge, C., Smith, A. U. and Parkes, A. S. Revival of spermatazoa after vitrification and dehydation at low temperatures. Nature 1949; 164:666.CrossRefGoogle Scholar
Bunge, R. G. and Sherman, J. K. Fertilizing capacity of frozen human spermatozoa. Nature 1953; 172:767–8.CrossRefGoogle ScholarPubMed
Di Santo, M., Tarozzi, N., Nadalini, M. and Borini, A. Human sperm cryopreservation: update on techniques, effect on DNA integrity, and implications for ART. Adv Urol 2012; 2012:854837.CrossRefGoogle ScholarPubMed
Isachenko, V., Isachenko, E., Katkov, I. I., et al. Cryoprotectant-free cryopreservation of human spermatozoa by vitrification and freezing in vapor: effect on motility, DNA integrity, and fertilization ability. Biol Reprod 2004; 71:1167–73.CrossRefGoogle ScholarPubMed
Spis, E., Bushkovskaia, A., Isachenko, E., et al. Conventional freezing vs. cryoprotectant-free vitrification of epididymal (MESA) and testicular (TESE) spermatozoa: Three live births. Cryobiology 2019; 90:100–2.CrossRefGoogle ScholarPubMed
Tao, Y., Sanger, E., Arpornas, S. and Leville, M. Human sperm vitification:the state of the art. Reprod Biol Endocrinol 2020; 18(1):17.CrossRefGoogle Scholar
Li, Y. X., Zhou, L., Lv, M. Q., et al. Vitrification and conventional freezing methods in sperm cryopreservation: A systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol 2019; 233:84–92.CrossRefGoogle ScholarPubMed
World Health Organization (WHO). Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. (Geneva, Switzerland: WHO, 2010).Google Scholar
AbdelHafez, F., Bedaiwy, M., El-Nashar, S. A., Sabanegh, E. and Desai, N. Techniques for cryopreservation of individual or small numbers of human spermatozoa: a systematic review. Hum Reprod Update 2009; 15:153–64.Google ScholarPubMed
Gangrade, B. K. Cryopreservation of testicular and epididymal sperm: techniques and clinical outcomes of assisted conception. Clinics 2013; 68 (Suppl 1):131–40.CrossRefGoogle ScholarPubMed
Cohen, J., Garrisi, G. J., Congedo-Ferrara, T. A., et al. Cryopreservation of single human spermatozoa. Hum Reprod 1997; 12:994–1001.CrossRefGoogle ScholarPubMed
Liu, S. and Li, F. Cryopreservation of single-sperm: where are we today? Reprod Biol Endocrinol 2020; 18(1):41.CrossRefGoogle ScholarPubMed
Desai, N. N., Blackmon, H. and Goldfarb, J. Single sperm cryopreservation on cryoloops: an alternative to hamster zona for freezing individual spermatozoa. Reprod Biomed Online 2004; 9:47–53.CrossRefGoogle ScholarPubMed
Coetzee, K., Ozgur, K., Berkkanoglu, M., Bulut, H. and Isikli, A. Reliable single sperm cryopreservation in Cell Sleepers for azoospermia management. Andrologia 2016; 48(2):203–10.CrossRefGoogle ScholarPubMed
Endo, Y., Fujii, Y., Shintani, K., et al. Single spermatozoon freezing using Cryotop. J Mamm Ova Res 2011; 28:47–52.CrossRefGoogle Scholar
Berkovitz, A., Miller, N., Silberman, M., Belenky, M. and Itsykson, P. A novel solution for freezing small numbers of spermatozoa using a sperm vitrification device. Hum Reprod 2018; 33(11):1975–83.CrossRefGoogle ScholarPubMed

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