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Chapter 18 - In Vitro Maturation of Immature Oocytes for IVM–IVF

from Section 3 - Gametes

Published online by Cambridge University Press:  07 August 2023

By
Satoshi Mizuno  and
Aisaku Fukuda
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Most laboratories use HEPES buffer solution for oocyte retrieval because it provides pH stability through buffering action. In contrast, there are laboratories that use workstations which provide CO₂ and temperature stability during oocyte collection. When this type of workstation is used, HEPES buffer solution is not required. In laboratories working without a dedicated workstation, the recovery and processing of oocytes should be done quickly to maintain optimal oocyte temperature and pH conditions.

Keywords

IVMimmature oocytefertility preservationPCOSOHSScancer
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 136 - 143
Publisher: Cambridge University Press
Print publication year: 2023

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References

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