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Chapter 17 - Oocyte Collection for IVF

from Section 3 - Gametes

Published online by Cambridge University Press:  07 August 2023

By
Marcela Calonge
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Oocyte retrieval is the starting point of a complex laboratory process that is the final aim of delivering a healthy baby. Exposure of oocytes to inappropriate conditions could potentially disrupt fertilization and early development. It is well accepted that minimizing stress during gamete and embryo their manipulation is critical for optimizing outcomes. The potential physicochemical stressors that can affect oocytes and embryos must be controlled to diminish possible negative effects. These include temperature control, maintaining osmolarity, pH, and protection from oxidative stress and toxic substances such as volatile organic compounds (VOC). Therefore, precautions must be taken to maintain adequate conditions to protect zygote and embryo homeostasis.

Keywords

oocyte retrievaloocytecumulus–oocyte complexfollicular fluid
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 130 - 135
Publisher: Cambridge University Press
Print publication year: 2023

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References

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