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Chapter 26 - Sperm Selection for ICSI by Viability

from Section 4 - Insemination/ICSI

Published online by Cambridge University Press:  07 August 2023

By
Markus H. M. Montag
Edited by
Markus H. M. Montag  and
Dean E. Morbeck
Markus H. M. Montag
Affiliation:
ilabcomm GmbH, St Augustin, Germany
Dean E. Morbeck
Affiliation:
Kindbody Inc, New York City
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Summary

Sperm motility is an important factor for successful fertilization and embryo development. If a patient presents only immotile sperm in the ejaculate or in a testicular sample, a viability test can help to identify among the immotile sperm those that are viable and suitable for intracytoplasmic sperm injection (ICSI). Different sperm viability tests have been introduced, and if they are applied properly, there is a good chance for successful treatment.

Keywords

hypo-osmotic swelling testlasertheophyllinespermselectionviabilityICSIsperm tail
Type
Chapter
Information
Principles of IVF Laboratory Practice
Laboratory Set-Up, Training and Daily Operation
 , pp. 196 - 200
Publisher: Cambridge University Press
Print publication year: 2023

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References

Oliveira, N. M., Sanchez, R. V., Fiesta, S. R., et al. Pregnancy with frozen–thawed and fresh testicular biopsy after motile and immotile sperm microinjection, using the mechanical touch technique to assess viability. Hum Reprod 2004; 19:262–5.Google Scholar
Jeyendran, R. S., van der Ven, H. H., Perez- Pelaez, M., Crabo, B. G. and Zaneveld, L. J. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 1984; 70:219–28.Google Scholar
Ved, S., Montag, M., Schmutzler, A., et al. Pregnancy following intracytoplasmic sperm injection of immotile spermatozoa selected by the hypo-osmotic swelling-test: a case report. Andrologia 1997; 29:241–2.Google ScholarPubMed
Sallam, H., Farrag, A., Agameya, A., et al. The use of a modified hypo-osmotic swelling test for the selection of viable ejaculated and testicular immotile spermatozoa in ICSI. Hum Reprod 2001; 16:272–6.Google Scholar
Montag, M., Rink, K., Delacrétaz, G. and van der Ven, H. Laser-induced immobilization and plasma membrane permeabilization in human spermatozoa. Hum Reprod 2000; 15:846–52.CrossRefGoogle ScholarPubMed
Aktan, T. M., Montag, M., Duman, S., et al. Use of a laser to detect viable but immotile spermatozoa. Andrologia 2004; 36:366–9.CrossRefGoogle ScholarPubMed
Nordhoff, V., Schüring, A. N., Krallmann, C., et al. Optimizing TESE-ICSI by laser-assisted selection of immotile spermatozoa and polarization microscopy for selection of oocytes. Andrology 2013; 1:67–74.Google Scholar
Yovich, J. L. Pentoxifylline: actions and applications in assisted reproduction. Hum Reprod 1993; 8:1786–91.CrossRefGoogle Scholar
Ebner, T., Tews, G., Mayer, R. B., et al. Pharmacological stimulation of sperm motility in frozen and thawed testicular sperm using the dimethylxanthine theophylline. Fertil Steril 2011; 96:1331–6.CrossRefGoogle ScholarPubMed

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