Book contents
- Single-Molecule Science
- Single-Molecule Science
- Copyright page
- Dedication
- Contents
- Contributors
- Foreword
- Preface
- Part I Super-Resolution Microscopy and Molecular Imaging Techniques to Probe Biology
- Part II Protein Folding, Structure, Confirmation, and Dynamics
- Part III Mapping DNA Molecules at the Single-Molecule Level
- 7 Observing Dynamic States of Single-Molecule DNA and Proteins Using Atomic Force Microscope
- 8 Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification
- Part IV Single-Molecule Biology to Study Gene Expression
- Index
- References
7 - Observing Dynamic States of Single-Molecule DNA and Proteins Using Atomic Force Microscope
from Part III - Mapping DNA Molecules at the Single-Molecule Level
Published online by Cambridge University Press: 05 May 2022
Book contents
- Single-Molecule Science
- Single-Molecule Science
- Copyright page
- Dedication
- Contents
- Contributors
- Foreword
- Preface
- Part I Super-Resolution Microscopy and Molecular Imaging Techniques to Probe Biology
- Part II Protein Folding, Structure, Confirmation, and Dynamics
- Part III Mapping DNA Molecules at the Single-Molecule Level
- 7 Observing Dynamic States of Single-Molecule DNA and Proteins Using Atomic Force Microscope
- 8 Atomic Force Microscopy and Detecting a DNA Biomarker of a Few Copies without Amplification
- Part IV Single-Molecule Biology to Study Gene Expression
- Index
- References
Summary
Biomolecules and biopolymers undergo conformational transitions during many biological processes. For example, some proteins are observed to have multiple intermediate states in the folding/unfolding pathways (Stigler et al., 2011; Yu et al., 2012); intrinsically disordered proteins can form diverse metastable structures (Neupane et al., 2014); functional proteins can often be switched between active and inactive states through conformational transitions (Yang et al., 2003; Hanson et al., 2007; Wijeratne et al., 2013); nucleosomes are able to regulate DNA unwrapping through their conformational transitions (Ngo et al., 2015). These dynamic states of DNA and proteins control their biological functions. Since force plays a fundamental role in many, if not all, biological systems, one way to reveal the dynamics of the molecules is to elucidate its intra- and intermolecular force, which can be used as a marker to capture information about their conformational changes.
- Type
- Chapter
- Information
- Single-Molecule ScienceFrom Super-Resolution Microscopy to DNA Mapping and Diagnostics, pp. 97 - 110Publisher: Cambridge University PressPrint publication year: 2022
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