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Four- and six-hour urinary albumin excretion is a valuable alternative to 24-h urinary albumin excretion in male db/db mice

Published online by Cambridge University Press:  01 January 2023

SA Nørgaard ,
FW Sand ,
DB Sørensen  and
H Søndergaard
SA Nørgaard*
Affiliation:
Pharmacology, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Måløv, Denmark Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 9, 2 Sal, 1870 Frederiksberg C, 1-62 Denmark
FW Sand
Affiliation:
Pharmacology, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Måløv, Denmark
DB Sørensen
Affiliation:
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Ridebanevej 9, 2 Sal, 1870 Frederiksberg C, 1-62 Denmark
H Søndergaard
Affiliation:
Pharmacology, Novo Nordisk A/S, Novo Nordisk Park 1, 2760 Måløv, Denmark
*
* Contact for correspondence: sissenoergaard@gmail.com
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Abstract

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In mouse (Mus musculus) models of diabetic nephropathy (DN), one of the most important read-outs is the 24-h urinary albumin excretion (UAE). The 24-h urine collection is usually performed by single housing mice in metabolic cages on wire mesh without enrichment. This is known to be stressful for the mice. Therefore, it was investigated if shorter urine collections would be sufficient to get reliable assessments of albuminuria. Twenty-one diabetic (C57BLKS-Leprdb/ db) and ten non-diabetic mice (C57BLKS-Leprdb/+) were placed in metabolic cages at 15 and 20 weeks of age (WoA) for 24 h. Urine samples were taken at 4, 6, 18 and 24 h and albumin and creatinine concentration were measured. Four- and 6-h UAE was found to correlate significantly with 24-h UAE. Furthermore, a significant correlation was found between 24-h UAE and albumin:creatinine ratio (ACR) in the 4-h sample. However, the strength of the correlation between ACR and 24-h UAE was weaker than between the 4- and 24-h UAE. This suggests that normalising to creatinine may not provide additional value to the 4-h urine collection. In conclusion, the strong correlation between 4- and 6-h UAE and 24-h UAE indicates that the collection period can be considerably reduced. This refinement could reduce stress and increase welfare of the db/db model and potentially be applied to other DN models.

Keywords

albuminuriaanimal welfaredb/db micediabetic nephropathymetabolic cagesrefinement
Type
Research Article
Information
Animal Welfare , Volume 29 , Issue 2 , May 2020 , pp. 143 - 149
Copyright
© 2020 Universities Federation for Animal Welfare

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