Infra-red thermography (IRT) is a non-invasive tool for measuring eye temperature as an indicator of stress and welfare in animals. Previous studies state that images are taken from 90° but do not specify a reference point or method of standardisation. The aims of the current study were to determine whether the position of the IRT camera has an impact on recorded temperature and which camera position is optimal for indicating stress in a mammal with anterolateral eyes. IRT images were taken from 90° to the nasal plane, eye and sagittal plane on the left side of the horses’ faces (n = 14) at eye level before and after exposure to a novel object. Distance and angle of measurement was standardised using ground markers. Temperature at each point of measurement was compared against heart rate variability. A significant difference was found between recorded temperature at all three of the points of measurement, both before and after the novel object test, suggesting that IRT camera position has an impact on eye temperature results. There was a significant strong positive correlation between eye temperature taken from 90° to the sagittal plane and heart rate variability, but no such correlation was observed from 90° to the nasal plane or eye. This suggests that a 90° angle in relation to the sagittal plane is the optimal position for taking eye temperature measurements using IRT, whereas 90° to the eye is commonly used. This study offers a validated protocol for using IRT to measure stress and welfare in mammals with anterolateral eyes.

Pig (Sus scrofa) production in Hungary provides a case study in how external pressures influence animal production, animal welfare and intensification. External pressures were explored in 24 in-depth, semi-structured interviews with Hungarian pig farmers operating either confinement or alternative systems. Confinement producers reported intense economic pressure because of a power imbalance with the large meat-processing companies that buy their animals. These companies, in the view of the farmers, can source internationally and largely dictate prices. When prices paid by the companies fall below the cost of production, farmers cannot respond by reducing production because of the long time-lags between breeding and marketing; and with their large investment in confinement buildings that are difficult to modify, farmers see little option except to reduce production costs further. Alternative farmers reported being more resilient to economic pressures because they sell into niche markets, use inexpensive technologies, and typically produce a diversity of agricultural products which buffer periods of low profit in any one commodity. The current regulatory system was seen as inadequate to protect animal welfare from economic pressure because it focuses on certain inputs rather than welfare outcomes, does not cover some important determinants of animal welfare, and does not accommodate certain realities of farming. Current subsidies were also seen as an inadequate remedy, and were viewed as inequitable because they are difficult for alternative producers to access. Consumer-choice options, while used by alternative producers, are not available in mainstream markets which demand uniform ‘commodity’ production. The economic constraints that influence animal welfare might be better mitigated by a regulatory system developed with greater consultation with producers, a more equitable subsidy programme, and more developed consumer-choice programmes.

In mouse (Mus musculus) models of diabetic nephropathy (DN), one of the most important read-outs is the 24-h urinary albumin excretion (UAE). The 24-h urine collection is usually performed by single housing mice in metabolic cages on wire mesh without enrichment. This is known to be stressful for the mice. Therefore, it was investigated if shorter urine collections would be sufficient to get reliable assessments of albuminuria. Twenty-one diabetic (C57BLKS-Leprdb/ db) and ten non-diabetic mice (C57BLKS-Leprdb/+) were placed in metabolic cages at 15 and 20 weeks of age (WoA) for 24 h. Urine samples were taken at 4, 6, 18 and 24 h and albumin and creatinine concentration were measured. Four- and 6-h UAE was found to correlate significantly with 24-h UAE. Furthermore, a significant correlation was found between 24-h UAE and albumin:creatinine ratio (ACR) in the 4-h sample. However, the strength of the correlation between ACR and 24-h UAE was weaker than between the 4- and 24-h UAE. This suggests that normalising to creatinine may not provide additional value to the 4-h urine collection. In conclusion, the strong correlation between 4- and 6-h UAE and 24-h UAE indicates that the collection period can be considerably reduced. This refinement could reduce stress and increase welfare of the db/db model and potentially be applied to other DN models.

Pregnant (dry) sows (Sus scrofa) are fed a rationed amount of feed to maintain healthy weight and production but this does not satisfy their hunger. This study measured the extent of feed restriction compared to sows’ desired intake. Forty-seven Large White × Landrace sows were housed in small groups with straw bedding and individual feeding stalls. Following three days on a standard ration of 2.5 kg, they were offered 10 kg a day of commercial dry sow feed for three days, split into four 2.5-kg meals a day which enabled individual intakes to be measured. This quantity was effectively ad libitum (maximum daily intake 9.4 kg). Mean (± SEM) intake per day over the three ad libitum days was 5.67 (± 0.24) kg, compared to the 2.5-kg standard ration. The ration thus provides less than half (44.1%) of sows’ desired intake. Behaviour on their third rationed day was compared with behaviour on the third day of ad libitum. Eating rate and the display of hunger-related behaviours, particularly following the morning feed, was greater under ration feeding; sows spent more time in the food stall and less in the straw bed, and more time active rather than resting. During ration-feeding sows also chewed and nosed more at straw bedding and pen equipment and used the drinker more after their morning meal than when they were fed ad libitum. Eating rate on the last rationed day was positively correlated with feed intake on each of the ad libitum days. Despite an EU requirement for fibre to be added to diets to ameliorate this problem, and the provision of straw bedding, hunger resulting from food restriction remains a welfare concern for dry sows.

Several established models in human and veterinary medicine exist to evaluate an individual health or disease status. Many of these seem unsuitable for further epidemiological research aimed at discovering underlying influential factors. As a case example for score development and choice, the present study analyses different approaches to scoring the foot health of Asian elephants (Elephas maximus) living in European facilities. Sum scores with varying degree of detail, and without or with a weighting method, were compared using descriptive statistics, ie kurtosis, skewness, Shannon entropy, total redundancy, their maximum and their actual ranges. With increasing score complexity, a higher level of differentiation was reached. In parallel, the distribution of score frequencies in the population shifted systematically: with the least complex scoring model the pattern indicated a severely unhealthy population with an opposite skew to a hypothetically healthy population, whereas the most complex scoring model indicated a mildly affected population with a skew corresponding to that expected for a healthy population. We propose the latter, in the form of the Particularised Severity Score (ParSev), which accounts for every nail and pad individually and weights the sub-scores by squaring, as the most relevant score for further investigations, either in assessing changes within an elephant population over time, or correlating foot health in epidemiological studies to potentially influencing factors. Our results emphasise the relevance of choosing appropriate scoring models for welfare-associated evaluations, due to implications for the applicability as well as the perceived welfare status of the test population.

Meat quality is not only influenced by breed but also rearing environment. The aim of this study was to evaluate the influence of different housing environments on growth performance, carcase traits, meat quality, physiological response pre-slaughter and fatty acid composition in two pig breeds. A total of 120 growing pigs at 60-70 days of age were arranged in a 2 × 2 factorial design with the breeds (Duroc × Landrace × Large White [D × L × LW] and Duroc × Landrace × Min pig [D × L × M]) and environmental enrichment (barren concrete floor or enriched with straw bedding) as factors. Each treatment was performed in triplicate with ten pigs per replicate. The pigs housed in the enriched environment exhibited a higher average daily gain, average daily feed intake, saturated fatty acid percentage and backfat depth than the pigs reared in the barren environment. Plasma cortisol levels were lower and growth hormone higher in enriched compared to barren pens. The D × L × M pigs showed lower cooking loss compared with the D × L × LW pigs. Moreover, the D × L × M pigs exhibited poor growth performance but had a better water-holding capacity. Only carcase traits and meat quality interaction effects were observed. We concluded that an enriched environment can reduce preslaughter stress and improve the growth performance of pigs and modulate the fatty acid composition of pork products.

In this study, an appropriate visual scoring system for foot-pad dermatitis was validated, considering the histologically measured depth of the inflammation zone and the histopathological grade (no lesion, mild lesion, ulcer). The aim being to evaluate whether the visual, macroscopic scoring of foot-pad dermatitis can represent the histological, microscopic findings. Two hundred Ross 308 broiler chicken feet (birds aged 39-42 fattening days) were collected at a slaughterhouse and scored macroscopically according to a modified version of the Welfare Quality® Assessment Protocol for Poultry. Afterwards, 200 histological slides (one per foot) were prepared, the extent of the inflammation measured and all slides scored by veterinarian pathologists using Michel et al's modified scheme. The statistical relationship between microscopic and macroscopic score and depth of inflammation were estimated via regression models. Increasing macroscopic score was found to be linked with an increase in microscopic score and the depth of inflammation. In particular, feet without lesions and feet with ulcers were identifiable using the macroscopic score. Macroscopic scoring of foot-pad dermatitis can mirror histological findings once certain limitations are taken into account (superficial lesions were not clearly identifiable). Foot-pad dermatitis is considered a useful indicator of animal welfare and our findings suggest that visual, macroscopic scoring could be a practicable assessment tool.

This study aimed to identify current weak points in animal welfare in Danish dairy production at herd level using the Welfare Quality® (WQ) protocol, and at national level using the Danish Animal Welfare Index (DAWIN) protocol. The DAWIN was developed as a monitoring tool for the welfare of the Danish dairy cow population, derived from the aggregation of DAWIN assessments at herd level. The DAWIN dairy cow protocol covers 29 measures (13 resource- and 16 animal-based measures) that were weighted and aggregated into a final overall population welfare score. A total of 3,591 cows from 60 dairy herds were assessed throughout 2015. Results from both the WQ and DAWIN were presented at six criteria levels in order to identify specific areas of concern relating to animal welfare at herd versus population level. Both protocols indicated a good general level of welfare across study herds, but also identified insufficient water supply as the main area of concern. In addition, resting comfort (ie time needed to lie down, collisions with barn equipment, cleanliness of rear body parts, animals lying outside of the designated lying area) and disease (in terms of the proportion of cows with chronically elevated somatic cell counts) were identified as problematic areas. The two assessment protocols both identified behavioural deficits, but in the WQ it was due to zero-grazing systems in contrast to the insufficient numbers of cow brushes in the DAWIN protocol. Despite differences in the aggregation, similar areas of concern were identified at criteria level.

In the wild, rabbits (Oryctolagus cuniculus) are social but territorial. Recent surveys suggest that over half of pet rabbits are housed singly. We aimed to compare welfare in single versus paired rabbits. We predicted singletons would show more abnormal or escape behaviour, greater stress responses to handling, and reduced body temperature (being unable to huddle with another individual when cold), but that pairs may show aggression. This study was conducted during winter at a rabbit-only rescue centre, and included 45 rabbits, comprising 15 housed singly and 15 pairs. Like most pet rabbits, they were housed either outdoors or in unheated outbuildings. Singletons were mostly in smaller enclosures than pairs. Home-pen observations (40 min) revealed bar-biting in 8/15 single rabbits compared with 0/30 of the paired ones. No other behavioural effects of social housing reached significance, and enclosure size showed no significant effects. Body temperature was significantly lower in singletons than pairs, with at least 0.5°C mean difference. On colder days, rabbits adopted compact postures more, and relaxed postures less frequently. After handling, pairs resumed normal behaviour significantly more quickly than singletons in the home-pen (3.0 [± 0.3] versus 8.3 [± 1.3] s, respectively). Aggression was never observed. The results indicate that social housing reduces bar-biting, aids thermoregulation, and may help buffer stress. Rabbit owners should be encouraged to meet the need for rabbits to be housed with an appropriate conspecific in a suitably large, sheltered enclosure.