In New Zealand there is a long history of research studies working with wild free-living brushtail possums (Trichosurus vulpecula) where euthanasia of large numbers of animals is frequently required. The most commonly used method for euthanising trapped possums is by blunt-force trauma. If possums being euthanised are potentially infected with bovine tuberculosis (TB), however, the blunt-force trauma method is associated with risks of researcher exposure to TB infection (eg risk of being scratched, bitten or blood-splattered). Here, we trial the use of a close-range shot to the head with a high-velocity air rifle as an alternative method for the euthanasia of cage-trapped possums. Our trial revealed that the air rifle method reduced the potential disease exposure risks to researchers by minimising blood-spatter area, and by completely avoiding the likelihood of both accidents due to animal handling and animal escapes. While both methods induced immediate unconsciousness leading to death, the air rifle method was quicker (ie shorter conscious handling and total time) and arguably had better animal welfare performance by significantly reducing the time to unconsciousness. Thus, we conclude that the air rifle method is suitably humane for possum euthanasia and should be considered when dealing with potentially TB-infected cage-trapped possums.

Moles are widely trapped as pests on farms and amenity land in Britain. Spring traps for killing mammals generally require welfare approval in the UK, but mole traps are exempt. Previous research demonstrated wide variation in the mechanical performance of mole traps. In this context, we aimed to produce new data on the welfare impact of kill-trapping moles in the field. We collected 50 moles trapped in southern England (November 2008-August 2009). Captures peaked during the peak in male breeding activity, when captures were almost exclusively male. Post mortem and x-ray (radiation) examinations were conducted to determine injuries and likely cause of death. No moles sustained damaged skulls or upper cervical vertebrae (which could cause unconsciousness immediately). The primary identifiable cause of death for all but one mole was acute haemorrhage; this contrasts with the findings of the only previous such study, in which only one mole showed clear evidence of haemorrhaging. Some moles may have asphyxiated although it was not possible to determine this. Moles most likely became unconscious before death, but times to unconsciousness, and death, can be determined only through killing trials and further investigation is urgently needed. This should be done through the spring traps approval process; this could improve the welfare standards of trapping for many thousands of moles each year. Mole trapping for long-term population control might be better targeted after the peak in male breeding activity, when females are more likely to be caught, but this would threaten the welfare of dependent young underground.

Social Network Analysis (SNA) enables the fine scale of animal sociality and population structure to be quantified. SNA is widely applied to questions relating to behavioural ecology but has seen little use in the application to zoo animal management, despite its clear potential. Investment in social bonds between individuals positively affects health status, welfare state, long-term fitness and lifetime reproductive output. Such positive affective states can be maintained consistently within captive situations if more information is known about the social preferences of the individuals that are kept. Disruption to social bonds may lead to impoverished welfare and stress to individuals which have seen their social support compromised. The patterning of social relationships between individuals also influences how space is utilised and how animals interact with resources provided for them. With more detailed knowledge of the social structure of a group or population, social groupings (for example, for captive breeding) can be specifically designed to minimise social stress. Likewise, enhancing the chances of successful reproduction can be achieved if we understand the role that each individual within a network plays and how these roles may impact on the behaviour of others. This paper discusses key aspects of SNA applicable to zoo-based researchers wishing to investigate the social lives of zoo animals. We present a review of how SNA can be used to assess social behaviour and highlight directions for future research. Our aim is to stimulate new research to ultimately improve our understanding of reproductive success, decision-making, group leadership, animal health and enclosure use. We conclude that what can be learned about the dynamics of social zoo-housed species using SNA can directly impact on husbandry decisions and help underpin excellent standards of animal welfare.

The restraint and sedation of wild animals has welfare implications, thus animal handling procedures should be well-informed and optimised to adhere to welfare standards. Furthermore, it is important that handling procedures should not cause future trap avoidance. This is of particular pertinence to European badgers (Meles meles), subject to extensive cage-trapping, relating to bovine tuberculosis epidemiology. We examined 4,288 capture/recapture events for 856 individual badgers, occurring between May 1999-September 2011, recording initial observed behaviour and reaction provoked by injection, on a scale ranging from still (0) to distressed/aggressive (3). Eighty-seven percent of adults and 76% of cubs were still (0) when approached initially and 75% of adults and 62% of cubs remained still when injected. Cubs exhibited significantly higher behavioural responses than adults, while female adults scored higher provoked scores than males. Importantly, the initial behaviour of an individual dictated its provoked response. Previous experience of capture was associated with lower subsequent behavioural response scores, while naïve badgers were most prone to score highly. Individuals first caught as cubs scored lower initial responses than those first caught as adults. Lower initial responses occurred in spring and summer and higher responses were associated with lice infestation. Behavioural criteria have potential to inform and optimise welfare in badger capture operations. This contributes to techniques allowing simple, non-invasive assessment of how wild animals in general respond to temporary restraint, where the psychological perception acts as the precursor to physiological stress.

This paper proposes a system that uses intrinsic study data to provide a clear visualisation of the stresses involved during the animal's life history that can be applied to all types of studies, even those not requiring invasive techniques. Thus, it provides an opportunity for researchers to identify and refine key events which impact on the welfare of an animal, and to explain clearly the totality of any necessary harms when justifying the research. Assessment of animal welfare depends on measurement of a number of parameters which will vary according to species, the animal's environment and the scientific procedure, all of which are inter-related. Currently, there are few tools to assess the effects of lifetime events on welfare or even, in some cases, to recognise that they have an impact on the level of suffering. A matrix to assess the combined effects of environment, experimental and contingent events on welfare has been applied, retrospectively, to programmes of work involving macaques (Macaca mulatta and M. fascicularis). Lifetime records, available for animals from their birth in the breeding colony through to experimental use in vaccine efficacy evaluation studies, were analysed as a robust validation test for the assessment matrix and refinement of the way in which information on these events is captured. A meaningful assessment method is required prospectively for project licence applications and retrospectively for licence review or decisions on re-use. The analysis will provide information that would support the application of refinements that would optimally enhance the lives of experimental animals.

Contemporary animal welfare thinking is increasingly emphasising the promotion of positive states. There is a need for existing assessment frameworks to accommodate this shift in emphasis. This paper describes extensions to the Five Domains model, originally devised to assess welfare compromise, that facilitate consideration of positive experiences that may enhance welfare. As originally configured, the model provided a systematic method for identifying compromise in four physical/functional domains (nutrition, environment, health, behaviour) and in one mental domain that reflects the animal's overall welfare state understood in terms of its affective experiences. The specific modifications described here now facilitate additional identification in each domain of experiences animals have which may be accompanied by positive affects that would enhance welfare. It is explained why the grading scale and indices for evaluating welfare compromise necessarily differ from those for assessing welfare enhancement. Also, it is shown that the compromise and enhancement grades can be combined to provide a single informative symbol, the scaled use of which covers the range from severe welfare compromise and no enhancement to no compromise and high-level enhancement. Adapted thus, the Five Domains model facilitates systematic and structured assessment of positive as well as negative welfare-related affects, the circumstances that give rise to them and potential interactions between both types of affect, all of which extend the utility of the model. Moreover, clarification of the extended conceptual framework of the model itself contributes to the growing contextual shift in animal welfare science towards the promotion of positive states whilst continuing to minimise negative states.

In some parts of the world, the laboratory pig (Sus scrofa) is often housed in individual, sterile housing which may impose stress. Our objectives were to determine the effects of isolation and enrichment on pigs housed within the PigTurn® — a novel penning system with automated blood sampling — and to investigate tear staining as a novel welfare indicator. Twenty Yorkshire × Landrace weaner pigs were randomly assigned to one of four treatments in a 2 × 2 factorial combination of enrichment (non-enriched [NE] or enriched [E]) and isolation (visually isolated [I] or able to see another pig [NI]). Pigs were catheterised and placed into the PigTurns® 48 h post recovery. Blood was collected automatically twice daily to determine white blood cell (WBC) differential counts and assayed for cortisol. Photographs of the eyes were taken daily and tear staining was quantified using a 0-5 scoring scale and Image-J software to measure stain area and perimeter. Behaviour was video recorded and scan sampled to determine time budgets. Data were analysed as an REML using the MIXED procedure of SAS. Enrichment tended to increase proportion of time standing and lying laterally and decrease plasma cortisol, tear-stain area and perimeter. There was a significant isolation by enrichment interaction. Enrichment given to pigs housed in isolation had no effect on plasma cortisol, but greatly reduced it in non-isolated pigs. Tear-staining area and perimeter were highest in the NE-I treatment compared to the other three treatments. Eosinophil count was highest in the E-NI treatment and lowest in the NE-I treatment. The results suggest that in the absence of enrichment, being able to see another animal but not interact may be frustrating. The combination of no enrichment and isolation maximally impacted tear staining and eosinophil numbers. However, appropriate enrichment coupled with proximity of another pig would appear to improve welfare.

Fractures occurring in conscious broiler chickens are painful and severely compromise animal welfare. The aim of this study was to investigate the effects of pre-slaughter handling procedures on the frequency of wing fractures. Wings were examined for fractures in 11,609 broilers, from 12 different flocks, slaughtered in two abattoirs: one using bi-phasic CO2 stunning (CS); and one using electric water-bath stunning (ES). The same broilers were examined: i) in lairage, representing fractures attributed to catching and transportation; ii) after evacuation of transport containers and shackling (only ES); and iii) post-stunning. The mean frequencies of wing fractures were: in the lairage; 0.8% (CS 0.73%, ES 0.88%); after shackling prior to stunning; 2.90% (only ES); and after stunning; 2.35% (CS 1.80%, ES 2.90%). Regardless of stunning method, significantly more fractures occurred during pre-slaughter handling at the abattoirs than during catching/transportation. The difference in prevalence between CS and ES was not significant. All fractures observed in the ES occurred in conscious animals, whereas in the CS it was not possible to distinguish between fractures occurring in conscious or stunned broilers. From a welfare perspective, fractures occurring on-farm/transport result in prolonged suffering and are thus considered more serious in risk assessments of broiler welfare, even though more fractures occur at the abattoir. Monitoring of wing fractures at abattoirs should be included as an indicator of broiler welfare.

In Australia, several macropod species are subjected to commercial harvesting, recreational hunting and population management, using both lethal and non-lethal measures. Some techniques for killing macropods can cause prolonged and/or severe suffering, and of particular concern is the welfare of remaining pouch young or young-at-foot, when females with dependent young are killed. Non-lethal methods are more widely supported by the general public and include reproductive control and relocation. These methods, however, also have significant associated welfare challenges. This review outlines the welfare concerns for each current method, and concludes that an accurate head-shot by an experienced shooter is least likely to inflict suffering. However, this assumes best practice shooting, which may not be representative of field conditions. Furthermore, many aspects of macropod control and killing still require significant research. These include, but are not limited to: accurate statistics for pouch young and young-at-foot mortality and morbidity in Australian states; data on field-based compliance with National Codes of Practice; safe and remote administration of reproductive control measures; and the impact of using dogs and trapping in wallaby destruction and hunting.

The flight distance from humans and the reaction of the mother to human handling of their offspring are measures that can be used to assess the quality of the human-animal relationship which could vary according to animals’ position in a group. The objective was to determine if the flight distance and the mother's reaction to human handling of her fawn during the first 24 h after birth differ according to pampas deer (Ozotoceros bezoarticus) hinds’ social rank. A complementary aim was to compare the mothers’ reaction to their fawns being handled relative to its sex. Studies were carried out on a semi-captive population. Animals were classed as high- or low-ranking individuals according to agonistic interactions between hinds recorded during autumn (breeding season) while animals received rations. In the first part of the study, the flight distance was determined in high- and low-ranked hinds. In the second, the minimum distance that the mother stayed from her fawn was recorded while the fawn was weighed and sexed during the first 24 h after birth, and the latency period for the dam to return with her fawn was also recorded. High-ranked hinds presented greater flight distance than low-ranked hinds. High-ranked hinds kept a greater distance from their fawns compared to low-ranked hinds and more high-than low-ranked hinds remained at a farther distance. In summary, high-ranked hinds seem to perceive humans as a greater threat, and thus be more fearful of them. The sex of the fawn did not affect the hinds’ reaction to human handling.

The Welfare Quality® (WQ) on-farm welfare assessment protocols for fattening pigs (n = 95 farms) and sows, as well as suckling piglets (n = 103 farms), were applied on Finnish farms. In order to identify distinct types of welfare problems (WPTs) Principal Component Analysis (PCA) was applied to the pooled animal-based items within both categories of animals. Measures describing suckling piglets did not contribute to the WPTs. The main WPTs (seemingly) reflected fighting in fattening pigs and lack of bedding in both fattening pigs and sows. The results imply that WQ includes biologically plausible shortlists of animal-based measures with decent to good internal consistency describing distinct types of welfare problems in growing pigs and in sows. The 20 descriptors of Qualitative Behavioural Assessment were analysed similarly to identify distinct mood types, which were named active positive, passive positive and passive positive behaviours. The different mood types had close to identical build-up in both fattening pigs and in sows and suckling piglets.

While concerns regarding the day-to-day management of domestic horses have been raised in relation to behaviour problems and welfare, most published studies have focused on the management of performance horses and less is known about the routine management of leisure horses and the prevalence of behavioural problems within this population. The objective of this study was to generate data on the day-to-day management of UK leisure horses and to quantify the prevalence of stable/stall-related and handling behaviour problems. A self-administered internet survey was used to collect individual horse-level data from a convenience sample of leisure horse owners. The survey was online for a year and respondents were asked to report on their routine over the previous week to minimise recall bias. The survey covered the horses’ stable and turn-out routine and environments, including opportunities for social contact with other horses. Respondents were also asked to rate the frequency their horse displayed 20 stable-related and handling behaviour problems. Data on 1,850 individual horses were collected. Stable-related and handling behaviour problems were displayed by 82% of horses sampled. The findings suggest a trend towards year-round stabling. The restriction in turn-out opportunities may have welfare costs for the horses involved. The high prevalence of stable-related and handling problems, including stereotypies, is a concern.

A common and painful management practice undertaken on most dairy farms is dehorning young calves (also called ‘disbudding’ when done on calves less than about two months of age). Despite much evidence the practice is painful, and effective means available to mitigate this pain, it is frequently performed without pain relief. The overall aim of this study was to describe different stakeholder views on the use of pain mitigation for disbudding and dehorning. Using an interactive, online platform, we asked participants whether or not they believed that calves should be disbudded and dehorned with pain relief and to provide reasons to support their choice. Participant composition was as follows: dairy producer or other farm worker (10%); veterinarian or other professional working with the dairy industry (7%); student, teacher or researcher (16%); animal advocate (9%); and no involvement with the dairy industry (57%). Of 354 participants, 90% thought pain relief should be provided when disbudding and dehorning. This support was consistent across all demographic categories suggesting the industry practice of disbudding and dehorning without pain control is not consistent with normative beliefs. The most common themes in participants’ comments were: pain intensity and duration, concerns about drug use, cost, ease and practicality and availability of alternatives. Some of the participants’ reasoning corresponded well with existing scientific evidence, but other reasons illustrated important misconceptions, indicating an urgent need for educational efforts targeted at dairy producers and dairy industry professionals advising these producers.

The field of welfare science and public concern for animal welfare is growing, with the focus broadening from animals on farms to those in zoos and aquaria. Bottlenose dolphins (Tursiops truncatus) are the most common captive cetaceans, and relevant regulatory standards are principally resource-based and regarded as minimum requirements. In this study, the farm animal Welfare Quality® assessment was adapted to measure the welfare of bottlenose dolphins, with a similar proportion of animal-based measures (58.3%). The ‘C-Well® ‘ assessment included eleven criterion and 36 species-specific measures developed in situ at three marine mammal zoological facilities, tested for feasibility and accuracy, and substantiated by published literature on wild and captive dolphins and veterinary and professional expertise. C-Well® scores can be calculated for each measure or combined to achieve an overall score, which allows for the comparison of welfare among individuals, demographics, and facilities. This work represents a first step in quantifying and systematically measuring welfare among captive cetaceans and can be used as a model for future development in zoos and aquaria, as well as a means to support benchmarking, industry best practices, and certification.

The energetic costs of animal movement change with body condition, although the consequences of this for foraging efficiency are rarely considered. We deployed externally attached devices to Magellanic penguins (Spheniscus magellanicus), known to increase the costs of swimming via increased drag in a consistent manner, and noted, however, that foraging behaviour and efficiency varied dramatically between years. We used our results to construct an energetics-based model and found that small increases in drag due to the attachment of externally attached tags predicted accelerating harm as prey availability decreased, which accorded with our observations. This explains earlier observations of differential breeding success in tagged versus untagged penguins in particular years, highlights the importance of understanding how animal body condition may affect population processes in general and advocates caution in interpretations of tag-derived data over variable environmental conditions.

This study aimed to establish associations between the environment and animal-based measures of welfare collected on 158 Finnish farms according to the Welfare Quality® systems for pigs. The data consisted of 95 welfare assessments in fattening pigs and 103 in sows, including suckling piglets. Principal Component Analysis had previously been applied to animal-based welfare measures (ABWM) and to the 20 descriptors of QBA to identify distinct types of welfare problems (WPT) and mood (MT), respectively. Generalised linear modeling was used to investigate environmental (space allowance, group size, feeding arrangement, floor type and use of enrichment or bedding) effects on WPT and MT scores. Those ABWMs not contributing to the major WPTs, but occurring on more than 40% of the farms, were considered important and used as outcome variables as well. The most important environmental determinants of pig welfare were space allowance for fattening pigs, group size in gestation and in the use of bedding for both fattening pigs and gestating sows. Bedding decreased tail biting and signs of fighting when used as a fairly thick layer for fattening pigs. In sows, the benefits of bedding, including less frustration and bursitis, required a smaller amount of material than in fattening pigs. An increasing space allowance was advantageous for fattening pigs, although signs of fighting increased in very spacious bedded pens. The positive effects of space, including a decrease of tail lesions and a more positive mood continued at least up to 1.5 m2 per fattening pig. Signs of resource shortage in sows increased with a growing group size according to a steepening curve.

High calf mortality rate is a significant problem facing semi-domestic reindeer (Rangifer tarandus) production around the world. Mortality rates, commonly due to predation, can range from 5 to 70%, which constitutes a great welfare concern. This study examined the influence of quantifiable maternal characteristics on reindeer calf survival. Data were compiled from 18 years’ worth of records on the survival of calves in the Cairngorm reindeer herd (Scottish Highlands, UK). Overall, mortality rate of calves (n = 635) in the herd was 34.9% to six months old, rising to 47.8% by one year old. For both Total Calf Survival (including perinatal losses) and Postnatal Calf Survival (excluding perinatal losses) of calves up to six months old, the only maternal trait found to significantly influence calf survival was the age of the cow at calving. Calves of very young and old cows showed higher mortality rates. Only 40% of the cows were associated with 77% of calf deaths and it was demonstrated that there were significant individual differences between cows in relation to their ability to consistently rear calves successfully. These findings can be applied to reduce calf mortality within herds, guiding selection towards females with successful reproductive histories and with ages falling in the prime productive range (3-11 years old). Additionally, annual variation had a highly significant influence on calf survival with rising mortality over the recorded period, indicating a role of environment on survival and an avenue for further research to investigate the impact of external factors, such as climate and pathogen load on post-natal loss.

In modern zoos, training should be an integral component of the animal care and management. The benefits of training include the opportunity for positive interactions with caretakers. This study was carried out with a group of vervet monkeys (Chlorocebus aethiops) housed at the Garda Zoological Park, Italy. Using focal animal sampling, we observed the behaviour performed by all group members from December 2007 to August 2008. The group took part in a training programme to be isolated in a familiar area before the subjects were included in a cognitive study. We collected behavioural data during a pre-training period to assess the social behaviour of the colony and during the training period to investigate the effects of the training programme on the behaviour of individuals. Additionally, a second phase of the study was conducted and training sessions with individual monkeys were video-recorded to determine the behaviour of animals during each training session and to thus confirm that they were suitable for participating in the procedure. Our results suggest that the training programme enriched the daily routine of these captive primates by increasing affiliative behaviours while decreasing agonistic behaviours. Furthermore, there was behavioural response variability among the individuals under the training procedure. However, all the individuals were trained to calmly enter a familiar area and be isolated from other members of the group. In conclusion, our findings highlight the importance of using positive reinforcement training to reduce the tension directly associated with potentially stressful procedures by allowing primates to participate voluntarily in these procedures. In addition, the training was found to be an enrichment tool for vervet monkeys.

Regarding broilers, Brazil is the third largest producer and the leading chicken meat exporter in the world. Independent certifications are required by some importers in the European Union (EU) to guarantee compliance with minimum welfare requirements. Our objective was to compare broiler chicken welfare in GLOBALGAP® certified (C) and non-certified (N) intensive farms in the State of Paraná, Brazil, using the Welfare Quality® protocol. Ten farms in each group were evaluated and data were transformed using scores that ranged from 0 to 100, where 100 is the best condition. Results suggested that farms adhered to minimum welfare standards, regardless of certification and that the adoption of standards based on overseas rules may have limitations for the improvement of animal welfare. In order to further improve broiler welfare in Brazil, more rigorous standards should be developed.

Sheep transport within Europe involves 9.5 million animals yearly, 63% of which travel over long journeys (> 8 h). Livestock transport, particularly over long journeys, gives rise to concern about the welfare of transported animals. The European Commission stimulates the development of market-oriented animal welfare standards for all phases of livestock production, providing an alternative to the ‘regulatory approach’. This study aimed to develop and test a new sheep welfare assessment protocol to be used following transport, irrespective of the journey purpose. The protocol included outcome (animal-based measures) and input variables (resource-based and management-based measures), being welfare-relevant aspects of both transport and unloading procedures. Weighted Cohen's Kappa and Fleiss’ Kappa index of agreement were calculated to evaluate the raters accuracy and the inter-observer reliability. Overall, good agreement levels were found. The protocol was tested on 40 commercial transports arriving at previously selected assembly centres and slaughterhouses in Italy and Greece. The protocol was found to be feasible when applied to commercial transports, allowing for a comprehensive and quick sheep welfare assessment during unloading, wthout impairing stockman work. Univariate analysis was carried out to evaluate associations between outcome and input variables. In this study, significant association between outcome measures and risk factors were identified when associated to unloading procedures but not to travel conditions. In collaboration with the relevant stakeholders, this protocol might be developed into a tool for routine checks for certification purposes and could provide direct feedback to all professionals involved in animal transportation on the weaknesses and strengths of their work.