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Influence of sources of dietary vitamin E on the maternal transfer of α-tocopherol to fetal and neonatal guinea pigs as determined by a stable isotopic technique

Published online by Cambridge University Press:  01 August 2008

N. Hidiroglou ,
R. Madere ,
L. R. McDowell  and
P. L. Toutain
N. Hidiroglou*
Affiliation:
Nutrition Research Division, Health Products and Food Branch, Health Canada, Banting Research Center (PL 2203C), Tunneys Pasture, Ottawa, Ontario, Canada K1A-OL2
R. Madere
Affiliation:
Nutrition Research Division, Health Products and Food Branch, Health Canada, Banting Research Center (PL 2203C), Tunneys Pasture, Ottawa, Ontario, Canada K1A-OL2
L. R. McDowell
Affiliation:
University of Florida, Department of Animal Sciences, Gainesville, Florida, USA
P. L. Toutain
Affiliation:
UMR INRA-Ecole Nationale Veterinaire de Toulouse de Physiopathologie et Toxicologie Expérimentales, Toulouse, F 31076, France
*
*Corresponding Author: Dr Nick Hidiroglou, fax +1 613 941 6182, email Nick_Hidiroglou@hc-sc.gc.ca
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Abstract

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The accepted biological potencies of vitamin E (United States Phamacopeia, 1985) for 1 mg all-rac-α-tocopheryl acetate (synthetic form) is 1·00 IU and that of 1 mg (RRR)-α-tocopheryl acetate (natural form) is 1·36 IU. In the present study, a stable isotopic (2H) technique was employed to evaluate the bioavailability of natural v. synthetic forms of vitamin E and to determine whether the potency of the forms is the stated relationship of 1·36:1·00 (RRR)-α-tocopheryl acetate:all-rac-α-tocopheryl acetate. Sixty female in-bred guinea pigs received either 40 or 80 mg vitamin E/kg diet with equal levels of (RRR)-α-tocopheryl acetate and all-rac-α-tocopheryl acetate throughout gestation and lactation. At late-term pregnancy (day 50 or 60) and during early lactation, dams and their corresponding fetuses or neonates were killed and various tissues collected for subsequent α-tocopherol analysis. Vitamin E analysis of fetal and neonatal tissues indicated a substantial transfer of 2H-labelled α-tocopherol across the placenta and through the mammary gland. Total α-tocopherol concentrations were significantly influenced by tissue type and dose level, but not by stage of gestation or lactation. The relative bioavailability (d3:d6) across fetal and neonatal tissues was on average 1·81:1·00, with a range from 1·62:1·00 to 2·01:1·00. Maternal tissues had a mean ratio of 1·77:1·00. A higher relative bioavailability (P≤0·05) was observed with natural compared with synthetic α-tocopherol as shown by a higher d3:d6 ratio in all tissues examined. Vitamin E was highest in colostrum on day 2 then declined through to day 5. Results from this present experiment further question the accepted biological potencies of natural:synthetic α-tocopheryl acetate of 1·36:1·00.

Keywords

Vitamin E sourcesBioavailabilityStable isotopesGuinea pig
Type
Research Article
Information
British Journal of Nutrition , Volume 89 , Issue 4 , April 2003 , pp. 455 - 466
Copyright
Copyright © The Nutrition Society 2003

References

Acuff, RV, Thedford, SS, Hidiroglou, NN, Papas, AM & Odom, TA Jr (1994) Relative bioavailability of RRR and all-rac-alpha-tocopheryl acetate in humans: studies using deuterated compounds. American Journal of Clinical Nutrition 60, 397–402.CrossRefGoogle ScholarPubMed
Behrens, W & Madere, R (1991) Tissue discrimination between dietary RRR-alpha tocopherol and all-rac-alpha tocopherols in rats. Journal of Nutrition 121, 454–459.CrossRefGoogle ScholarPubMed
Burton, GW & Ingold, KU (1993) Biokinetics of vitamin E using deuterated tocopherols. In Vitamin E in Health and Disease, pp. 329–344 [Packer, L and Fuchs, J, editors]. New York: Marcel Dekker.Google Scholar
Burton, GW, Traber, MG, Acuff, RV, Walters, DN, Kayden, H, Hughes, L & Ingold, KU (1998) Human plasma and tissue alpha tocopherol concentrations in response to supplementation with deuterated natural and synthetic vitamin E. American Journal of Clinical Nutrition 67, 669–684.CrossRefGoogle ScholarPubMed
Burton, GW, Webb, A & Ingold, KU (1985) Rapid lipid extraction with SDS for vitamin E/lipid ratios. Lipids 20, 29–39.CrossRefGoogle Scholar
Butcher, JR & Roberts, RJ (1981) Alpha-tocopherol (vitamin E) content of lung, liver and blood in the newborn rat and human infant. Influence of hyperoxia. Pediatrics 98, 806–811.Google Scholar
Canadian Council of Animal Care (1993) Guide to the Care and Use of Experimental Animals, 2nd ed. [Olfert, ED, Cross, BM and McWilliam, AA, editors]. Ottawa, Ontario, Canada: Bradda Publishing.Google Scholar
Chiswick, ML, Johnson, M & Toner, N (1982) Vitamin E and intravascular hemorrhage in the newborn. Annals New York Academy of Science 393, 109–118.Google Scholar
Chung, YK, Mahan, DC & Lepine, AJ (1992) Efficacy of dietary D-alpha-toco-pherol and DL-alpha-tocopheryl acetate for weanling pigs. Journal of Animal Science 70, 2485–2492.CrossRefGoogle ScholarPubMed
Ehrenkranz, RA, Ablow, RC & Warshaw, JB (1979) Prevention of bronchopulmonary dysplasia with vitamin E administration during the acute stages of respiratory distress syndrome. Journal of Pediatrics 95, 873–885.CrossRefGoogle ScholarPubMed
Ehrenkranz, RA, Bonta, BW, Ablovi, RC & Warshaw, JB (1978) Amelioration of bronchopulmonary dysplasia after vitamin E administration: a preliminary report. New England Journal of Medicine 299, 564–570.CrossRefGoogle ScholarPubMed
Engelhardt, ER (1977) Plasma and tissue levels of dietary radiotocopherols in the Harp Seal (Phoca Groenlandica). Canadian Journal of Physiology and Pharmacology 55, 601–608.Google Scholar
Fand, I & McNally, WP (1981) Whole body localization of 14C-tocopheryl acetate in the rat following oral adminstration. Archives International Pharmacodynamics Therapeutics 250, 4–17.Google Scholar
Health Canada (1995) Guidelines for the composition and clinical testing of formulas for preterm infants. Report of an ad hoc Expert Consultation, pp. 1–78. Ottawa, Ontario, Canada: Minister of Supply and Services Canada.Google Scholar
Hidiroglou, N, Laflamme, L & McDowell, LR (1988a) Blood plasma and tissue concentrations of vitamin E in beef cattle as influenced by supplementation of various tocopherol compounds. Journal of Animal Science 66, 3227–3234.CrossRefGoogle ScholarPubMed
Hidiroglou, N, McDowell, LR, Papas, AM, Antapli, M & Wikinson, NS (1992) Bioavailability of vitamin E compounds in lambs. Journal of Animal Science 70, 2556–2561.CrossRefGoogle ScholarPubMed
Hidiroglou, N, McDowell, LR & Pastrana, R (1988b) Bioavailability of various vitamin E compounds in sheep. International Journal of Vitamin Nutrition Research 58, 189–197.Google ScholarPubMed
Hidiroglou, N, Madere, R & McDowell, L (2001) Maternal transfer of vitamin E to fetal and neonatal guinea pigs utilizing a stable isotopic technique. Nutrition Research 21, 771–783.CrossRefGoogle Scholar
Hung, SSO, Moon, TW, Hilton, JW & Slinger, SJ (1982) Uptake, transport and distribution of DL-alpha-tocopheryl acetate compared to D-alpha tocopherol in rainbow trout. Journal of Nutrition 112, 1590–1599.CrossRefGoogle ScholarPubMed
Ingold, KU, Burton, GW, Foster, DO, Hughes, L, Lindsay, DA & Webb, A (1987) Biokinetics of and discrimination between dietary RRR and SRR-alpha tocopherols in the male rat. Lipids 22, 163–172.CrossRefGoogle ScholarPubMed
Johnson, NL, Quinn, GE, Abassi, S, Geodes, I, Bowen, FW & Bhutani, V (1995) Severe retinopathy of prematurity in infants with birth weights less than 1250 grams: Incidence and outcome of treatment with pharmacologic serum levels of vitamin E in addition to cryopathy from 1985 to 1991. Journal of Pediatrics 127, 632–639.CrossRefGoogle Scholar
Kelly, FJ (1993) Free radical disorders of preterm infants. British Medical Bulletin 49, 668–678.CrossRefGoogle ScholarPubMed
Kelly, FJ, Rodgers, W, Handel, J, Smith, S & Hall, MA (1990) Time course of vitamin E repletion in the premature infant. British Journal of Nutrition 63, 631–638.Google Scholar
Kelly, FJ, Safavi, M & Cheeseman, KH (1992) Tissue α-tocopherol status during late fetal and early neonatal life of the guinea pig. British Journal of Nutrition 67, 457–462.CrossRefGoogle ScholarPubMed
Leth, T & Sondergaard, H (1983) Biological activity of all-rac-alpha-tocopherol and RRR-alpha tocopherol determined by three different bioassays. International Journal of Vitamin and Nutrition Research 53, 297–311.Google Scholar
Mahan, DC, Kim, YY & Stuart, RL (2000) Effect of vitamin E sources (RRR- or all-rac-alpha tocopherol acetate) and levels on sow reproductive performance, serum, tissue, and milk alpha tocopherol contents over a five-parity period, and the effects on pregnancy. Journal of Animal Science 78, 110–119.Google Scholar
Mino, M & Nishino, H (1973) Fetal and maternal relationship in serum vitamin E level. Journal of Nutrition Science and Vitaminology 19, 475–482.CrossRefGoogle ScholarPubMed
Njeru, CA, McDowell, LR, Wilkinson, NS, Linda, SB & Williams, SN (1994) Pre- and postpartum supplemental DL-alpha tocopheryl acetate effects on placental and mammary vitamin E transfer in sheep. Journal of Animal Science 72, 1636–1640.CrossRefGoogle ScholarPubMed
Ogihara, T, Nishida, Y, Miki, M & Mino, M (1985) Comparative changes in plasma and red blood cell alpha tocopherol after administration of DL-alpha tocopheryl acetate and D-alpha tocopherol (1985). Journal of Nutrition Science and Vitaminology 31, 169–177.Google Scholar
Ostrea, EM, Balun, JE, Winkler, R & Porter, T (1986) Influence of breast-feeding on the restoration of the low serum concentration of vitamin E and β-carotene in the newborn infant. American Journal of Obstetrics and Gynecology 154, 1014–1017.CrossRefGoogle ScholarPubMed
Schlotke, B, Busch, L & Koch, F (1978) Untersuchungen zum einflub vitamin-E-armer ernahrung bei sauen wahrend der graviditat auf den vitamin-E status de ferkel in der neugebore-nenphase (Studies on the effect of vitamin E deficient diet in sows during pregnancy on the vitamin E status of newborn piglets). Zentralblatt fur Veterinarmedizin 25, 474–478.CrossRefGoogle Scholar
Speer, ME, Blifeld, C, Rudolph, AJ, Chadda, P, Holbein, B & Hittner, H (1984) Intraventricular hemorrhage and vitamin E in the very low-birth-weight infant: Evidence for efficacy of early intramuscular vitamin E administration. Pediatrics 74, 1107–1112.Google Scholar
Traber, MG (1996) Biokinetics of vitamin E. In Handbook of Antioxidants, pp. 43–61 [Cadenas, E and Packer, L, editors]. New York: Marcel Dekker, Inc.Google Scholar
United States Pharmacopeia (1985) The United States Pharmacopeia, 21st revised ed. Rochville, MD: United States Pharmacopeial Convention.Google Scholar
Weiser, H & Vecchi, M (1981) Stereoisomers of alpha-tocopheryl acetate characterization of the samples by physico-chemical methods and determination of biological activities in the rat resorption-gestation test. International Journal of Vitamin and Nutrition Research 51, 100–113.Google Scholar
Weiser, H, Riss, G & Kormann, AW (1996) Biodiscrimination of the eight alpha tocopherol stereoisomers results in preferential accumulation of the four 2R forms in tissues and plasma of rats. Journal of Nutrition 125, 2539–2549.CrossRefGoogle Scholar
Zhang, Z-H, Qi-Huo, Z & Yue, H (1987) Maternal–fetal transfer and developmental changes in permeability of blood–brain barrier for vitamin E in rat. Chinese Medical Journal 100, 731–734.Google Scholar