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Mechanism underlying the inhibitory effect of high calcium carbonate intake on iron bioavailability from ferrous sulphate in anaemic rats

Published online by Cambridge University Press:  09 March 2007

K. J. H. Wienk ,
J. J. M. Marx ,
A. G. Lemmens ,
E. J. Brink ,
R. Van Der Meer  and
A. C. Beynen
K. J. H. Wienk
Affiliation:
Department of Laboratory Animal Science, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands
J. J. M. Marx
Affiliation:
Department of Internal Medicine, Utrecht University Academic Hospital, Utrecht, The Netherlands
A. G. Lemmens
Affiliation:
Department of Laboratory Animal Science, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands
E. J. Brink
Affiliation:
Unilever Research Laboratorium, Vlaardingen, The Netherlands
R. Van Der Meer
Affiliation:
Netherlands Institute for Dairy Research, Ede, The Netherlands
A. C. Beynen
Affiliation:
Department of Laboratory Animal Science, Utrecht University, PO Box 80166, 3508 TD, Utrecht, The Netherlands
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Abstract

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The influence of high CaCO3, intake on the bioavailability of Fe from FeSO4, was assessed during Fe repletion of rats with Fe-deficiency-induced anaemia. Fe-deficient rats with a mean blood haemoglobin concentration of 4. 1 mmol/l were fed on purified Fe-adequate diets containing either 6.2 or 25.0g CaCO,/kg (ten rats per group). Haemoglobin repletion after 14 d was significantly depressed by high CaCO, intake (9.5 v. 9.8 mmol/l for high and low CaCO, intake respectively; P = 0·03), as was apparent Fe retention (367 v. 552 µg/d during days 5–7, P<0·001; 146 v. 196 µg/d during days 19–21, P < 0·001). The concentration of Fe in the liquid phase of the proximal half of the small intestine was significantly lower in the high-CaCO, group (3.71 v. 520 µg/g digesta; P = 0-02). Mucosal uptake and mucosal transfer of Fe were determined with orally administered 59Fe and Cr as a non-absorbable marker. Mucosal transfer was significantly diminished by CaCO, loading (90 v. 100% of mucosal uptake; P = 0·04), whereas mucosal uptake was not. 59Fe retention values at 14 d after administration were not significantly different (57.6 v. 51.9%; P = 0·14). Fe contents of liver and spleen were significantly decreased by high compared with low CaCO, intake (879 v. 590 pg Fe in liver, P < 0·001; 92 v. 63pg Fe in spleen, P <0·001). It is concluded that high intake of CaCO, depresses Fe bioavailability in rats. The CaCO,-induced decrease in Fe solubility in the digesta probably was associated with an increased efficiency of mucosal Fe uptake so that the amount of mucosal uptake remained unaltered. The CaCO,-induced decrease in Fe transfer through the mucosal cytoplasm and/or basolateral membrane may have been responsible for the concurrent decrease in Fe bioavailability.

Keywords

Non-haem FeCalcium carbonate intake
Type
Effect of calcium carbonate on iron bioavailability
Information
British Journal of Nutrition , Volume 75 , Issue 1 , January 1996 , pp. 109 - 120
Copyright
Copyright © The Nutrition Society 1996

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