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Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease

Published online by Cambridge University Press:  14 August 2017

Li Zhang ,
Qi-Fang Song ,
Jing-Jie Jin ,
Ping Huang ,
Zhou-Ping Wang ,
Xiao-Fei Xie ,
Xiao-Qiong Gu ,
Xue-Juan Gao  and
Hong-Ling Jia
Li Zhang
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Qi-Fang Song
Affiliation:
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, Guangdong, China
Jing-Jie Jin
Affiliation:
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China
Ping Huang
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Zhou-Ping Wang
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Xiao-Fei Xie
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Xiao-Qiong Gu
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Xue-Juan Gao*
Affiliation:
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China
Hong-Ling Jia*
Affiliation:
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China
*
#Correspondence to: X.-J. Gao and H.-L. Jia, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, 601 Huangpu Ave W, The 2nd Science and Technology Building, Room 914, Guangzhou 510632, China. Tel: +86 208 522 1071; Fax: +86 208 522 1071; E-mail: tgaoxj@jnu.edu.cn; jiahongling@aliyun.com
#Correspondence to: X.-J. Gao and H.-L. Jia, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, 601 Huangpu Ave W, The 2nd Science and Technology Building, Room 914, Guangzhou 510632, China. Tel: +86 208 522 1071; Fax: +86 208 522 1071; E-mail: tgaoxj@jnu.edu.cn; jiahongling@aliyun.com
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Abstract

Background

Kawasaki disease, which is characterised by systemic vasculitides accompanied by acute fever, is regularly treated by intravenous immunoglobulin to avoid lesion formation in the coronary artery; however, the mechanism of intravenous immunoglobulin therapy is unclear. Hence, we aimed to analyse the global expression profile of serum exosomal proteins before and after administering intravenous immunoglobulin.

Methods

Two-dimensional electrophoresis coupled with mass spectrometry analysis was used to identify the differentially expressed proteome of serum exosomes in patients with Kawasaki disease before and after intravenous immunoglobulin therapy.

Results

Our analysis revealed 69 differential protein spots in the Kawasaki disease group with changes larger than 1.5-fold and 59 differential ones in patients after intravenous immunoglobulin therapy compared with the control group. Gene ontology analysis revealed that the acute-phase response disappeared, the functions of the complement system and innate immune response were enhanced, and the antibacterial humoral response pathway of corticosteroids and cardioprotection emerged after administration of intravenous immunoglobulin. Further, we showed that complement C3 and apolipoprotein A-IV levels increased before and decreased after intravenous immunoglobulin therapy and that the insulin-like growth factor-binding protein complex acid labile subunit displayed reverse alteration before and after intravenous immunoglobulin therapy. These observations might be potential indicators of intravenous immunoglobulin function.

Conclusions

Our results show the differential proteomic profile of serum exosomes of patients with Kawasaki disease before and after intravenous immunoglobulin therapy, such as complement C3, apolipoprotein A-IV, and insulin-like growth factor-binding protein complex acid labile subunit. These results may be useful in the identification of markers for monitoring intravenous immunoglobulin therapy in patients with Kawasaki disease.

Keywords

Kawasaki diseaseintravenous immunoglobulinexosomeproteomics
Type
Original Articles
Information
Cardiology in the Young , Volume 27 , Issue 9 , November 2017 , pp. 1786 - 1796
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Copyright
© Cambridge University Press 2017 

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Footnotes

*

Li Zhang, Qi-Fang Song, and Jing-Jie Jin contributed equally to this work.

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