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Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease

Published online by Cambridge University Press:  14 August 2017

Li Zhang ,
Qi-Fang Song ,
Jing-Jie Jin ,
Ping Huang ,
Zhou-Ping Wang ,
Xiao-Fei Xie ,
Xiao-Qiong Gu ,
Xue-Juan Gao  and
Hong-Ling Jia
Li Zhang
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Qi-Fang Song
Affiliation:
Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University, Guangzhou 510632, Guangdong, China
Jing-Jie Jin
Affiliation:
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China
Ping Huang
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Zhou-Ping Wang
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Xiao-Fei Xie
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Xiao-Qiong Gu
Affiliation:
Guangzhou Women and Children’s Medical Center, Guangzhou 510120, Guangdong, China
Xue-Juan Gao*
Affiliation:
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China
Hong-Ling Jia*
Affiliation:
Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China
*
#Correspondence to: X.-J. Gao and H.-L. Jia, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, 601 Huangpu Ave W, The 2nd Science and Technology Building, Room 914, Guangzhou 510632, China. Tel: +86 208 522 1071; Fax: +86 208 522 1071; E-mail: tgaoxj@jnu.edu.cn; jiahongling@aliyun.com
#Correspondence to: X.-J. Gao and H.-L. Jia, Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University, 601 Huangpu Ave W, The 2nd Science and Technology Building, Room 914, Guangzhou 510632, China. Tel: +86 208 522 1071; Fax: +86 208 522 1071; E-mail: tgaoxj@jnu.edu.cn; jiahongling@aliyun.com
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Abstract

Background

Kawasaki disease, which is characterised by systemic vasculitides accompanied by acute fever, is regularly treated by intravenous immunoglobulin to avoid lesion formation in the coronary artery; however, the mechanism of intravenous immunoglobulin therapy is unclear. Hence, we aimed to analyse the global expression profile of serum exosomal proteins before and after administering intravenous immunoglobulin.

Methods

Two-dimensional electrophoresis coupled with mass spectrometry analysis was used to identify the differentially expressed proteome of serum exosomes in patients with Kawasaki disease before and after intravenous immunoglobulin therapy.

Results

Our analysis revealed 69 differential protein spots in the Kawasaki disease group with changes larger than 1.5-fold and 59 differential ones in patients after intravenous immunoglobulin therapy compared with the control group. Gene ontology analysis revealed that the acute-phase response disappeared, the functions of the complement system and innate immune response were enhanced, and the antibacterial humoral response pathway of corticosteroids and cardioprotection emerged after administration of intravenous immunoglobulin. Further, we showed that complement C3 and apolipoprotein A-IV levels increased before and decreased after intravenous immunoglobulin therapy and that the insulin-like growth factor-binding protein complex acid labile subunit displayed reverse alteration before and after intravenous immunoglobulin therapy. These observations might be potential indicators of intravenous immunoglobulin function.

Conclusions

Our results show the differential proteomic profile of serum exosomes of patients with Kawasaki disease before and after intravenous immunoglobulin therapy, such as complement C3, apolipoprotein A-IV, and insulin-like growth factor-binding protein complex acid labile subunit. These results may be useful in the identification of markers for monitoring intravenous immunoglobulin therapy in patients with Kawasaki disease.

Keywords

Kawasaki diseaseintravenous immunoglobulinexosomeproteomics
Type
Original Articles
Information
Cardiology in the Young , Volume 27 , Issue 9 , November 2017 , pp. 1786 - 1796
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Copyright
© Cambridge University Press 2017 

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Footnotes

*

Li Zhang, Qi-Fang Song, and Jing-Jie Jin contributed equally to this work.

References

1. Burns, JC, Glodé, MP. Kawasaki syndrome. Lancet 2004; 364: 533–544.Google Scholar
2. Kawasaki, T, Kosaki, F, Okawa, S, Shigematsu, I, Yanagawa, H. A new infantile acute febrile mucocutaneous lymph node syndrome (MLNS) prevailing in Japan. Pediatrics 1974; 54: 271–276.Google Scholar
3. Lin, MT, Sun, LC, Wu, ET, Wang, JK, Lue, HC, Wu, MH. Acute and late coronary outcomes in 1073 patients with Kawasaki disease with and without intravenous γ-immunoglobulin therapy. Arch Dis Child 2015; 100: 542–547.Google Scholar
4. Makino, N, Nakamura, Y, Yashiro, M, et al. Descriptive epidemiology of Kawasaki disease in Japan, 2011-2012: from the results of the 22nd nationwide survey. J Epidemiol 2015; 25: 239–245.CrossRefGoogle ScholarPubMed
5. Heuclin, T, Dubos, F, Hue, V, et al. Increased detection rate of Kawasaki disease using new diagnostic algorithm, including early use of echocardiography. J Pediatr 2009; 155: 695–699.Google Scholar
6. Ho, CL, Fu, YC, Lin, MC, Jan, SL. Early immunoglobulin therapy and outcomes in Kawasaki disease: a nationwide cohort study. Medicine (Baltimore) 2015; 94: e1544.CrossRefGoogle ScholarPubMed
7. Patel, RM, Shulman, ST. Kawasaki disease: a comprehensive review of treatment options. J Clin Pharm Ther 2015; 40: 620–625.Google Scholar
8. Ballow, M. The IgG molecule as a biological immune response modifier: mechanisms of action of intravenous immune serum globulin in autoimmune and inflammatory disorders. J Allergy Clin Immunol 2011; 127: 315–323.Google Scholar
9. Zhou, C, Huang, M, Xie, L, Shen, J, Xiao, T, Wang, R. IVIG inhibits TNF-α-induced MMP9 expression and activity in monocytes by suppressing NF-κB and P38 MAPK activation. Int J Clin Exp Pathol 2015; 8: 15879–15886.Google Scholar
10. Pap, E, Pállinger, E, Pásztói, M, Falus, A. Highlights of a new type of intercellular communication: microvesicle-based information transfer. Inflamm Res 2009; 58: 1–8.Google Scholar
11. Lakkaraju, A, Rodriguez-Boulan, E. Itinerant exosomes: emerging roles in cell and tissue polarity. Trends Cell Biol 2008; 18: 199–209.Google Scholar
12. Mathivanan, S, Ji, H, Simpson, RJ. Exosomes: extracellular organelles important in intercellular communication. J Proteomics 2010; 73: 1907–1920.Google Scholar
13. Yoon, YJ, Kim, OY, Gho, YS. Extracellular vesicles as emerging intercellular communicasomes. BMB Rep 2014; 47: 531–539.Google Scholar
14. Inal, JM, Kosgodage, U, Azam, S, Stratton, D, Antwi-Baffour, S, Lange, S. Blood/plasma secretome and microvesicles. Biochim Biophys Acta 2013; 1834: 2317–2325.Google Scholar
15. Kosaka, N, Iguchi, H, Yoshioka, Y, Takeshita, F, Matsuki, Y, Ochiya, T. Secretory mechanisms and intercellular transfer of microRNAs in living cells. J Biol Chem 2010; 285: 17442–17452.CrossRefGoogle ScholarPubMed
16. Hwang, I, Ki, D. Receptor-mediated T cell absorption of antigen presenting cell-derived molecules. Front Biosci 2011; 16: 411–421.Google Scholar
17. Zhou, DH, Yuan, ZG, Zhao, FR, et al. Modulation of mouse macrophage proteome induced by Toxoplasma gondii tachyzoites in vivo. Parasitol Res 2011; 109: 1637–1646.Google Scholar
18. Wu, G, Dawson, E, Duong, A, Haw, R, Stein, L. ReactomeFIViz: a Cytoscape app for pathway and network-based data analysis. Version 2 F1000Res 2014; 3: 146.Google Scholar
19. Yu, HR, Kuo, HC, Sheen, JM, et al. A unique plasma proteomic profiling with imbalanced fibrinogen cascade in patients with Kawasaki disease. Pediatr Allergy Immunol 2009; 20: 699–707.Google Scholar
20. Boguski, MS, Elshourbagy, N, Taylor, JM, Gordon, JI. Rat apolipoprotein A-IV contains 13 tandem repetitions of a 22-amino acid segment with amphipathic helical potential. Proc Natl Acad Sci U S A 1984; 81: 5021–5025.Google Scholar
21. Kalogeris, TJ, Rodriguez, MD, Tso, P. Control of synthesis and secretion of intestinal apolipoprotein A-IV by lipid. J Nutr 1997; 127: 537S–543S.CrossRefGoogle ScholarPubMed
22. Weinberg, RB, Cook, VR, DeLozier, JA, Shelness, GS. Dynamic interfacial properties of human apolipoproteins A-IV and B-17 at the air/water and oil/water interface. J Lipid Res 2000; 41: 1419–1427.CrossRefGoogle ScholarPubMed
23. Canales, A, Benedi, J, Bastida, S, et al. The effect of consuming meat enriched in walnut paste on platelet aggregation and thrombogenesis varies in volunteers with different apolipoprotein A4 genotype. Nutr Hosp 2010; 25: 746–754.Google Scholar
24. Wong, WM, Hawe, E, Li, LK, et al. Apolipoprotein AIV gene variant S347 is associated with increased risk of coronary heart disease and lower plasma apolipoprotein AIV levels. Circ Res 2003; 92: 969–975.Google Scholar
25. Fofanova-Gambetti, OV, Hwa, V, Kirsch, S, et al. Three novel IGFALS gene mutations resulting in total ALS and severe circulating IGF-I/IGFBP-3 deficiency in children of different ethnic origins. Horm Res 2009; 71: 100–110.Google Scholar
26. Domené, HM, Scaglia, PA, Martínez, AS, et al. Heterozygous IGFALS gene variants in idiopathic short stature and normal children: impact on height and the IGF system. Horm Res Paediatr 2013; 80: 413–423.Google Scholar
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