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Transmission of Helicobacter spp. A challenge to the dogma of faecal-oral spread

Published online by Cambridge University Press:  15 May 2009

A. Lee ,
J. G. Fox ,
G. Otto ,
E. Hegedus Dick  and
S. Krakowka
A. Lee*
Affiliation:
The University of New South Wales, Kensington, Australia, 2033
J. G. Fox
Affiliation:
Massachusetts Institute of Technology, Boston, Massachusetts 02139
G. Otto
Affiliation:
Massachusetts Institute of Technology, Boston, Massachusetts 02139
E. Hegedus Dick
Affiliation:
The University of New South Wales, Kensington, Australia, 2033
S. Krakowka
Affiliation:
Ohio State University, Columbus, Ohio 43210
*
Professor Adrian Lee, School of Microbiology & Immunology, University of New South Wales, P.O. Box 1, Kensington, NSW, Australia, 2033
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Summary

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Faecal oral spread is claimed by many to be the mode of transmission of the gastric pathogen Helicobacter pylori. This idea is based not on experimental data but because the epidemiology of H. pylori infection resembles that of other pathogens known to be spread by the faecal-oral route. This is in spite of the observation that no-one has been successful in culturing H. pylori from human stool. In this study, a series of transmission experiments are reported on animals infected with the gastric spirilla, Helicobacter felis and ‘Gastrospirillum hominis’. Germfree mice and rats infected with H. felis did not transmit their infection to uninoculated mice despite prolonged contact in the same cage nor could the bacterium be isolated from their intestinal contents. This was confirmed in specific pathogen free mice where infected dams did not pass the helicobacter to their progeny. Similarly, mice infected with a human isolate of ‘Gastrospirillum hominis’ did not transmit the infection while in close contact with uninoculated mice. In contrast, in a limited series of experiments, both H. pylori and H. felis were transmitted from infected gnotobiotic Beagle puppies to uninfected animals in the same enclosure. In addition, the gastric mucus from a cat with indigenous ‘Gastrospirillum’-like organisms was infectious for mice, whereas faecal content from the same animal was not. It is suggested that the difference between the murine and canine experiments is that the dogs are more likely to have oral-oral contact than rodents. Unlike dogs, mice and rats do not vomit and are coprophagous. It is concluded that the case for faecal-oral spread of Helicobacter species is ‘not proven’ and that the inter-oral route is more likely.

Type
Research Article
Information
Epidemiology & Infection , Volume 107 , Issue 1 , August 1991 , pp. 99 - 109
Copyright
Copyright © Cambridge University Press 1991

References

REFERENCES

1.Marshall, BJ, Warren, JR. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet 1984; i: 1331–5.Google Scholar
2.Graham, DY. Campylobacter pylori and peptic ulcer disease. Gastroenterol 1989; 96: 615–52.CrossRefGoogle ScholarPubMed
3.Fox, JG, Correa, P, Taylor, NS, et al. Campylobacter pylori associated gastritis and immune response in a population at increased risk of gastric carcinoma. American Gastroenterol 1989; 89: 775–81.Google Scholar
4.Mitchell, HM, Bohane, TD, Berkowicz, TD, Hazell, SL, Lee, A. Antibody to Campylobacter pylori in families of index children with gastrointestinal illness due to C. pylori. Lancet 1987; ii: 681–2.CrossRefGoogle Scholar
5.Berkowicz, J, Lee, A. Person to person spread of Campylobacter pylori. Lancet 1987; ii: 680–1.CrossRefGoogle Scholar
6.Mitchell, HM, Lee, A, Carrick, J. An increased incidence of Campylobacter pylori infection in gastroenterologists: further evidence to support person to person transmission of C. pylori. Scand J Gastroenterol 1989; 24: 396–400.CrossRefGoogle ScholarPubMed
7.Graham, DY. Helicobacter pylori: Future directions in research. In: Malfertheiner, P, Ditschuneit, H. eds. Helicobacter pylori. Gastritis and peptic ulcer. Berlin. Heidelberg: Springer-Verlag. 1990; 463–70.CrossRefGoogle Scholar
8.Jones, DM, Curry, A. The genesis of coccoid forms of Helicobacter pylori. In: Malfertheiner, P, Ditschuneit, H. eds. Helicobacter pylori. Gastritis and peptic ulcer. Berlin. Heidelberg: Springer-Verlag. 1990; 29–37.CrossRefGoogle Scholar
9.Mai, UE, Shahamat, M, Colwell, RR. Survival of Helicobacter pylori in a dormant but viable stage. Enfermedades Digestivas 1990; 78 (suppl): 17.Google Scholar
10.Lee, A, Hazell, SL, O'rourke, J, Kouprach, S. Isolation of a spiral-shaped bacterium from the cat stomach. Infect Immun 1988; 56: 2843–50.CrossRefGoogle ScholarPubMed
11.Paster, BJ, Lee, A, Dewhirst, FE, Fox, JG, Yordoff, LA, Ferrero, R. The phylogeny of Helicobacter felis nov., a spiral-shaped bacterium isolated from the gastric mucosa of a cat. Helicobacter mustelae, and related bacteria. Int J Syst Bact 1991; 41: 31–8.CrossRefGoogle Scholar
12.McNulty, CAM, Dent, JC, Curry, A, et al. New spiral bacterium in gastric mucosa. J Clin Pathol 1989; 42: 585–91.CrossRefGoogle ScholarPubMed
13.Dent, JC, McNulty, CAM, Uff, JC, Wilkinson, SP, Gear, MWL. Spiral organisms in the gastric antrum. Lancet 1987; ii: 242–4.Google Scholar
14.Dick, E, Lee, A, Watson, G, O'rourke, . The isolation and investigation of stomach-associated spiral/helical shaped bacteria from humans and other animals using the mouse. J Med Micro 1989; 29: 55–62.CrossRefGoogle ScholarPubMed
15.Hazell, SL, Borody, TJ, Gal, A. Campylobacter pyloridis gastritis I: detection of urease as a marker of bacterial colonization and gastritis. Am J Gastroenterol 1987; 82: 292–6.Google ScholarPubMed
16.Lee, A, Fox, JG, Otto, G, Murphy, J. A small animal model of human Helicobacter pylori active chronic gastritis. Gastroenterology 1990; 99: 1315–23.CrossRefGoogle ScholarPubMed
17.Fox, JG, Lee, A, Otto, G, Taylor, NS, Murphy, JC. Helicobacter felix gastritis in gnotobiotic rats: An animal model of H. pylori gastritis. Infect Immun. In press.Google Scholar
18.Krakowka, S, Long, D, Mezza, R, Mador, RA, Kuestner, A. Derivation and maintenance of gnotobiotic dogs. Lab Amin Sci 1978; 28: 178–81.Google ScholarPubMed
19.Radin, MJ, Eaton, KA, Morgan, DR, Lee, A, Ott, G, Fox, JG. Helicobacter pylori gastric infection in gnotobiotic Beagle dogs. Infect Immun 1990; 58: 2606–12.CrossRefGoogle ScholarPubMed
20.Lee, A, Krakowka, S, Fox, LG, Otto, KA, Murphy, J. Helicobacter felis as a cause of lymphoreticular hyperplasia in the dog stomach. A confounding factor in canine toxicological studies. Vet Path. Submitted for publication.Google Scholar
21.Krakowka, S, Morgan, D, Kraft, W, Leunk, R. Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect Immun 1987; 55: 2789–96.CrossRefGoogle ScholarPubMed
22.Lambert, JR, Borromeo, M, Pinkard, KJ, Turnet, H, Chapman, CB, Smith, ML. Colonization of gnotobiotic piglets with Campylobacter pyloridis – An animal model? J Infect Dis 1987; 155: 1344.CrossRefGoogle ScholarPubMed
23.Albenque, M, Tall, F, Dabis, F, Megraud, F. Epidemiological study of Helicobacter pylori transmission from mother to child in Africa. Enfermedades Digestivas 1990; 78 (suppl 1): 48.Google Scholar
24.Krajden, S, Fuska, M, Anderson, J. Examination of human stomach biopsies, saliva, and dental plaque for Campylobacter pylori. J Clin Micro 1989; 27: 1397–8.CrossRefGoogle ScholarPubMed
25.Dye, KR, Marshall, BJ. Frierson, Pambianco, DJ, McCallum, RW. Campylobacter pylori colonizing heterotopic gastric tissue in the rectum. Am J Clin Pathol 1990; 93: 144–7.CrossRefGoogle ScholarPubMed
26.De Cothi, GA, New bold, JM, O'Connor, HJ. Campylobaeter-like organisms and heterotopic gastric mucosa in Meckel's diverticula. J Clin Pathol 1989; 42: 132–4.CrossRefGoogle ScholarPubMed
27.Klein, P. High prevalence of Campylobacter pylori (CP) infection in poor and rich Peruvian children determined by 13C urea breath test. Gastroenterology 1990; 96: 260.Google Scholar
28.Lambert, JR, Schembri, M, Lin, SK, et al. High prevalence of Helicobacter pylori antibodies in institutionalized adults. Aust Microbiol 1990; 11: 252.Google Scholar