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Fast and reliable detection of doubled-haploids in Asparagus officinalis by stringent RAPD-PCR

Published online by Cambridge University Press:  05 January 2004

K. EIMERT ,
G. REUTTER  and
B. STROLKA
K. EIMERT
Affiliation:
Department of Botany, State Research Institute Geisenheim, Geisenheim, Germany
G. REUTTER
Affiliation:
Department of Botany, State Research Institute Geisenheim, Geisenheim, Germany
B. STROLKA
Affiliation:
Deutsche Spargelhochzucht Station, Spargelhof Gast, Alt-Mölln, Germany
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Abstract

A stringent RAPD-PCR protocol was developed for reliable distinction at an early stage between somatic and doubled-haploid androgenic regenerates emerging from asparagus anther culture by using decamer primers with a high G/C content and/or primers with a dinucleotide sequence. RAPD-PCR was used to test 87 putative doubled-haploids from seven donor plants of different varieties and breeding lines, with differing genetic backgrounds. All of them differed from the anther donor, which suggests they had the desired doubled-haploid constitution, and demonstrates the effectiveness of the in vitro protocol used. This simple, reliable, fast, and comparatively cheap method eliminates the need for discriminating testcrosses and, thus, can significantly accelerate asparagus breeding programmes.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 141 , Issue 1 , August 2003 , pp. 73 - 78
Copyright
2003 Cambridge University Press

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