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Prediction and copy number variation identification of ZNF146 gene related to growth traits in Chinese cattle

Published online by Cambridge University Press:  10 August 2022

X. T. Ding Open the ORCID record for X. T. Ding [Opens in a new window] ,
X. Liu ,
X. M. Li ,
Y. F. Wen ,
J. W. Xu ,
W. J. Liu ,
Z. M. Li ,
Z. J. Zhang ,
Y. N. Chai  and
H. L. Wang
...Show all authors
X. T. Ding
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China
X. Liu
Affiliation:
Henan Provincial Animal Husbandry General Station, Zhengzhou, Henan 450008, People's Republic of China
X. M. Li
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China College of Animal Science and Technology, Xinjiang Agriculture University, Urumqi, Xinjiang 830052, People's Republic of China
Y. F. Wen
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China
J. W. Xu
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China
W. J. Liu
Affiliation:
College of Animal Science and Technology, Xinjiang Agriculture University, Urumqi, Xinjiang 830052, People's Republic of China
Z. M. Li
Affiliation:
Henan Provincial Animal Husbandry General Station, Zhengzhou, Henan 450008, People's Republic of China
Z. J. Zhang
Affiliation:
Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, People's Republic of China
Y. N. Chai
Affiliation:
Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, People's Republic of China
H. L. Wang
Affiliation:
Jiaxian Animal Husbandry Bureau, Jiaxian, Henan 467100, People's Republic of China
B. W. Cheng
Affiliation:
Jiaxian Animal Husbandry Bureau, Jiaxian, Henan 467100, People's Republic of China
S. H. Liu
Affiliation:
Jiaxian Animal Husbandry Bureau, Jiaxian, Henan 467100, People's Republic of China
B. Hou
Affiliation:
Jiaxian Animal Husbandry Bureau, Jiaxian, Henan 467100, People's Republic of China
Y. J. Huang
Affiliation:
Jiaxian Animal Husbandry Bureau, Jiaxian, Henan 467100, People's Republic of China
J. G. Li
Affiliation:
Jiaxian Animal Husbandry Bureau, Jiaxian, Henan 467100, People's Republic of China
L. J. Li
Affiliation:
Jiaxian Animal Husbandry Bureau, Jiaxian, Henan 467100, People's Republic of China
G. J. Yang
Affiliation:
Jiaxian Animal Husbandry Bureau, Jiaxian, Henan 467100, People's Republic of China
Z. F. Qi
Affiliation:
Jiaxian Animal Husbandry Bureau, Jiaxian, Henan 467100, People's Republic of China
F. Y. Chen
Affiliation:
Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, People's Republic of China
Q. T. Shi
Affiliation:
Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, People's Republic of China
E. Y. Wang
Affiliation:
Institute of Animal Husbandry and Veterinary Science, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, People's Republic of China
C. Z. Lei
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China
H. Chen
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China
B. R. Ru*
Affiliation:
Henan Provincial Animal Husbandry General Station, Zhengzhou, Henan 450008, People's Republic of China
Y. Z. Huang
Affiliation:
College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China
*
Authors for correspondence: B. R. Ru, E-mail: brr908@163.com; Y. Z. Huang, E-mail: hyzsci@nwafu.edu.cn
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Abstract

The great demographic pressure brings tremendous volume of beef demand. The key to solve this problem is the growth and development of Chinese cattle. In order to find molecular markers conducive to the growth and development of Chinese cattle, sequencing was used to determine the position of copy number variations (CNVs), bioinformatics analysis was used to predict the function of ZNF146 gene, real-time fluorescent quantitative polymerase chain reaction (qPCR) was used for CNV genotyping and one-way analysis of variance was used for association analysis. The results showed that there exists CNV in Chr 18: 47225201-47229600 (5.0.1 version) of ZNF146 gene through the early sequencing results in the laboratory and predicted ZNF146 gene was expressed in liver, skeletal muscle and breast cells, and was amplified or overexpressed in pancreatic cancer, which promoted the development of tumour through bioinformatics. Therefore, it is predicted that ZNF146 gene affects the proliferation of muscle cells, and then affects the growth and development of cattle. Furthermore, CNV genotyping of ZNF146 gene was three types (deletion type, normal type and duplication type) by Real-time fluorescent quantitative PCR (qPCR). The association analysis results showed that ZNF146-CNV was significantly correlated with rump length of Qinchuan cattle, hucklebone width of Jiaxian red cattle and heart girth of Yunling cattle. From the above results, ZNF146-CNV had a significant effect on growth traits, which provided an important candidate molecular marker for growth and development of Chinese cattle.

Keywords

Association analysisCNVFunction predictionGrowth traitsZNF146 gene
Type
Animal Research Paper
Information
The Journal of Agricultural Science , Volume 160 , Issue 5 , October 2022 , pp. 404 - 412
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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Footnotes

*

These authors contributed equally to this work.

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