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Suitability of bronopol preservative treated milk for fatty acid determination

Published online by Cambridge University Press:  17 March 2011

Gillian Butler  and
Sokratis Stergiadis
Gillian Butler*
Affiliation:
Nafferton Ecological Farming Group, Newcastle University
Sokratis Stergiadis
Affiliation:
Nafferton Ecological Farming Group, Newcastle University
*
*For correspondence; e-mail: gillian.butler@ncl.ac.uk
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Abstract

This work aimed to test if milk preserved with bronopol can be reliably used for fatty acid determination. Dairy production and milk quality are often monitored regularly to assess performance and contribute to selection indices. With evidence that fat composition can be influenced by selective breeding, there might be an interest in using samples collected in routine testing to evaluate individual cow fatty acid profiles, contributing to breeding indices. However, most recording services use a preservative such as bronopol and there is no published record if this influences subsequent fatty acid analysis. This study used milk from an oil seed supplementation trial, generating a wide range of milk fatty acid profiles, to test if the concentration of 31 individual fatty acids determined by GC were influenced by bronopol. Provided preserved samples are subsequently frozen, milk treated with bronopol can reliably be used to evaluate fatty acid composition in most cases; however bronopol might influence a few long-chain fatty acids present in relatively low concentrations. This is one small step towards simplifying milk compositional analysis but it could ultimately streamline the inclusion of milk fat quality into breeding indices, either with a view to ‘healthier’ milk or potentially reducing methane output and the environmental impact of dairy production.

Keywords

milkfatty acidspreservativebronopol
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 2 , May 2011 , pp. 220 - 225
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

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References

Bertrand, JA 1996 Influence of Shipping Container, Preservative, and Breed on Analysis of Milk Components of Shipped Samples. Journal of Dairy Science 79 (1) 145–148CrossRefGoogle Scholar
Butler, G, Nielsen, JH, Slots, T, Seal, C, Eyre, MD, Sanderson, R & Leifert, C 2008 Fatty acid and fat-soluble antioxidant concentrations in milk from high- and low-input conventional and organic systems: seasonal variation. Journal of the Science of Food and Agriculture 88 (8) 1431–1441CrossRefGoogle Scholar
Carroll, SM, DePeters, EJ, Taylor, SJ, Rosenberg, M, Perez-Monti, H & Capps, VA 2006 Milk composition of Holstein, Jersey, and Brown Swiss cows in response to increasing levels of dietary fat. Animal Feed Science and Technology 131 (3–4) 451–473CrossRefGoogle Scholar
Chilliard, Y, Martin, C, Rouel, J & Doreau, M 2009 Milk fatty acids in dairy cows fed whole crude linseed, extruded linseed, or linseed oil, and their relationship with methane output. Journal of Dairy Science 92 (10) 5199–5211CrossRefGoogle ScholarPubMed
Collomb, M, Bisig, W, Bütikofer, U, Sieber, R, Bregy, M & Etter, L 2008 Seasonal variation in the fatty acid composition of milk supplied to dairies in the mountain regions of Switzerland. Dairy Science and Technology 88 (6) 631–647CrossRefGoogle Scholar
Collomb, M, Bisig, W, Bütikofer, U, Sieber, R, Bregy, M & Etter, L 2008 Fatty acid composition of mountain milk from Switzerland: Comparison of organic and integrated farming systems. International Dairy Journal 18 976–982CrossRefGoogle Scholar
Dewhurst, RJ 2005 Targets for milk fat research: nutrient, nuisance or nutraceutical? Journal of Agricultural Science 143 359–367CrossRefGoogle Scholar
Dewhurst, RJ, Tweed, JKS, Davies, DWR & Fisher, WJ 2002 Effects of legume silages on the concentration of a-linolenic acid in milk. In 13th International Silage Conference, pp. 136–137. Ayr, ScotlandGoogle Scholar
Ellis, KA, Innocent, G, Grove-White, D, Cripps, P, McLean, WG, Howard, CV & Mihm, M 2006 Comparing the fatty acid composition of organic and conventional milk. Journal of Dairy Science 89 (6) 1938–1950CrossRefGoogle ScholarPubMed
Jensen, R 2002 The composition of bovine milk lipids: January 1995 to December 2000. Journal of Dairy Science 85 295–350CrossRefGoogle ScholarPubMed
Kelsey, J, Corl, B, Collier, R & Bauman, D 2003 The effect of breed, parity and stage of lactation on conjugated linoleic acid (CLA) in milk fat from dairy cows. Journal of Dairy Science 86 2588–2597CrossRefGoogle ScholarPubMed
Kennelly, J 1996 The fatty acid composition of milk fat as influenced by feeding oilseeds. Animal Feed Science and Technology 60 137–152CrossRefGoogle Scholar
Kramer, JKG & Zhou, J 2001 Conjugated linoleic acid and octadecenoic acids: Extraction and isolation of lipids. European. Journal of Lipid Science and Technology 103 (9) 594–6003.0.CO;2-R>CrossRefGoogle Scholar
Lawless, F, Stanton, C, L'Escop, P, Devery, R, Dillon, P & Murphy, JJ 1999 Influence of breed on bovine milk cis-9, trans-11-conjugated linoleic acid content. Livestock Production Science 62 (1) 43–49CrossRefGoogle Scholar
Malinowski, E, Smulski, S, Gehrke, M, Klossowska, A, Arczyanska, A & Kaczmarowski, M 2008 Effect of storage conditions and preservation with Bronopol on somatic cell count with the DeLaval cell counter in cow milk. Medycyna Weterynaryjna 64 (11) 1299–1303Google Scholar
Monardes, HG, Moore, RK, Corrigan, B & Rioux, Y 1996 Preservation and storage mechanisms for raw milk samples for use in milk-recording schemes. Journal of Food Protection 59 (2) 151–154CrossRefGoogle ScholarPubMed
Pickard, RM, Beard, AP, Seal, CJ & Edwards, SA 2008 Neonatal lamb vigour is improved by feeding docosahexaenoic acid in the form of algal biomass during late gestation. Animal 2 (8) 1186–1192CrossRefGoogle ScholarPubMed
Rego, OA, Rosa, HJD, Regalo, SM, Alves, SP, Alfaia, CMM, Prates, JAM, Vouzela, CM & Bessa, RJB 2008 Short Communication: Seasonal changes of CLA isomers and other fatty acids of milk fat from grazing dairy herds in the Azores. Journal of the Science of Food and Agriculture 88 1855–1859CrossRefGoogle Scholar
Rubio, R, Berruga, MI & Molina, A 2009 Effect of preservatives on immunoenzymatic methods for the analysis of aflatoxin M1 in ewe's milk. Milchwissenschaft 64 (4) 431–433Google Scholar
Sanchez, A, Sierra, D, Luengo, C, Corrales, JC, Morales, CT, Contreras, A & Gonzalo, C 2005 Influence of storage and preservation on Fossomatic cell count and composition of goat milk. Journal of Dairy Science 88 (9) 3095–3100CrossRefGoogle ScholarPubMed
Schennink, A, Heck, JML, Bovenhuis, H, Visker, MHPW, Van Valenberg, HJF & Van Arendonk, JAM 2008 Milk fatty acid unsaturation: Genetic parameters and effects of stearoyl-CoA desaturase (SCD1) and Acyl CoA: Diacylglycerol acyltransferase 1 (DGAT1). Journal of Dairy Science 91 (5) 2135–2143CrossRefGoogle ScholarPubMed
Shepherd, JA, Waigh, RD & Gilbert, P 1988 Antibacterial action of 2-bromo-2-nitropropane-1,3-diol (Bronopol). Antimicrobial Agents and Chemotherapy 32 (11) 1693–1698CrossRefGoogle ScholarPubMed
Sierra, D, Sanchez, A, Contreras, A, Luengo, C, Corrales, JC, de la fe, C, Guirao, I, Morales, CT & Gonzalo, C 2009 Short communication: Effect of storage and preservation on total bacterial counts determined by automated flow cytometry in bulk tank goat milk. Journal of Dairy Science 92 (10) 4841–4845CrossRefGoogle ScholarPubMed
Slots, T, Butler, G, Leifert, C, Kristensen, T, Skibsted, LH & Nielsen, JH 2009 Potentials to differentiate milk composition by different feeding strategies. Journal of Dairy Science 92 2057–2066CrossRefGoogle ScholarPubMed
Soyeurt, H, Dardenne, P, Gillon, A, Croquet, C, Vanderick, S, Mayeres, P, Bertozzi, C & Gengler, N 2006 Variation in fatty acid contents of milk and milk fat within and across breeds. Journal of Dairy Science 89 (12) 4858–4865CrossRefGoogle ScholarPubMed
Sukhija, PS & Palmquist, DL 1988 Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry 36 (6) 1202–1206CrossRefGoogle Scholar