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Effect of cleaning procedure and hygienic condition of milking equipment on bacterial count of bulk tank milk

Published online by Cambridge University Press:  04 March 2011

Luciana Bava ,
Maddalena Zucali ,
Anna Sandrucci ,
Milena Brasca ,
Laura Vanoni ,
Lucio Zanini  and
Alberto Tamburini
Luciana Bava*
Affiliation:
Dipartimento di Scienze Animali, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy
Maddalena Zucali
Affiliation:
Dipartimento di Scienze Animali, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy
Anna Sandrucci
Affiliation:
Dipartimento di Scienze Animali, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy
Milena Brasca
Affiliation:
Istituto di Scienze delle Produzioni Alimentari, CNR, via Celoria 2, 20133 Milano, Italy
Laura Vanoni
Affiliation:
Istituto di Scienze delle Produzioni Alimentari, CNR, via Celoria 2, 20133 Milano, Italy
Lucio Zanini
Affiliation:
Associazione Regionale Allevatori della Lombardia, viale Kennedy 30, 26013 Crema, Italy
Alberto Tamburini
Affiliation:
Dipartimento di Scienze Animali, Università degli Studi di Milano, via Celoria 2, 20133 Milano, Italy
*
*For correspondence: luciana.bava@unimi.it
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Abstract

The aim of the study was to describe the characteristics of cleaning procedures for milking equipment applied in intensive dairy farms in Lombardy (Italy) and to study their relationships with bacterial count of bulk milk and hygienic condition of milking machine components. A group of 22 dairy farms was visited twice (winter and summer) in order to collect bulk tank milk and post-rinse water samples and swabs from liners and milk receiver. Samples were analysed to determine: standard plate count (SPC), laboratory pasteurization count (LPC), psychrotrophic bacteria count (PBC), coliform count (CC) and Escherichia coli. Cleaning procedures were monitored using electronic milk flow meters with specific software for the measurement of the duration of each cleaning phase, circulating solution temperature and electrical conductivity, turbulence and water filling percentage of pipelines. The results showed that farms classified as high and low milk total bacteria count significantly differed both in terms of liners and receiver bacterial contamination and in terms of water temperature reached during the detergent phase of cleaning milking equipment. Significant positive correlations were found among total bacteria count in milk and bacterial contamination of the liners. Maximum water temperature reached during the cleaning cycle of milking equipment was very low (34·4±8·9°C on average); most of the observations (88·6%) corresponded to water temperatures <45°C. Cleaning temperature was related to psychrotrophic bacteria count of milk and post-rinse water and coliform count in liners. Routine check and regulation of water temperature during the washing phase of the milking machine can be a simple and effective way to control one of the main risk factors for bacteriological quality of bulk tank milk.

Keywords

Milking equipmentcleaning proceduremilkbacteria count
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 2 , May 2011 , pp. 211 - 219
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

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References

Chambers, J V 2002 The microbiology of raw milk. In: Dairy Microbiology Handbook 3rd Edn (Ed. Robinson, RK) p. 40. New York, USA: John Wiley & Sons Inc.Google Scholar
Elmoslemany, AM, Keefe, GP, Dohoo, IR & Jayarao, BM 2009a Risk factors for bacteriological quality of bulk tank milk in Prince Edward Island dairy herds. Part 1: Overall risk factors. Journal of Dairy Science 92 2634–2643CrossRefGoogle ScholarPubMed
Elmoslemany, AM, Keefe, GP, Dohoo, IR & Jayarao, BM 2009b Risk factors for bacteriological quality of bulk tank milk in Prince Edward Island dairy herds. Part 2: Bacteria count-specific risk factors. Journal of Dairy Science 92 2644–2652Google Scholar
European Commission 2004 Regulation (EC) No 853/2004 of the European Parliament and of The Council of 29 April 2004 laying down specific hygiene rules for on the hygiene of foodstuffs. Official Journal of the European Communities L139 55–205Google Scholar
Holm, C, Mathiasen, T & Jespersen, L 2004 A flow cytometric technique for quantification and differentiation of bacteria in bulk tank milk. Journal of Applied Microbiology 97 935–941CrossRefGoogle ScholarPubMed
Jayarao, BM, Pillai, SR, Sawant, AA, Wolfgang, DR & Hegde, NV 2004 Guidelines for monitoring bulk tank milk somatic cell and bacterial counts. Journal of Dairy Science 87 3561–3573Google Scholar
Kelly, PT, O'Sullivan, K, Berry, DP, More, SJ, Meaney, WJ, O'Callaghan, EJ & O'Brien, B 2009 Farm management factors associated with bulk tank total bacterial count in Irish dairy herds during 2006/2007. Irish Veterinary Journal 62 36–42Google Scholar
Keefe, G & Elmoslemany, A 2007 Consumer acceptance of fluid milk after raw milk selection using bulk tank bacteriologic and somatic cell count criteria. National Mastitis Council Annual Meeting Proceedings 218–219Google Scholar
Keown, J & Kononoff, PJ 2006 Producing milk with a low bacterial count. Neb guide. http://elkhorn.unl.edu/epublic/pages/publicationD.jsp?publicationId=676 Accessed 15 November 2010Google Scholar
Murphy, SC & Boor, KJ 2000 Source and causes of high bacteria counts in raw milk an abbreviated review. http://www.riboprinter.cornell.edu/cals/foodsci/extension/upload/BactRawRev.doc. Accessed 15 November 2010Google Scholar
Perkins, NR, Kelton, DF, Hand, KJ, MacNaughton, G, Berke, O & Leslie, KE 2009 An analysis of the relationship between bulk tank milk quality and wash water quality on dairy farms in Ontario, Canada. Journal of Dairy Science 92 3714–3722CrossRefGoogle ScholarPubMed
Regione, Lombardia 2007 Circolare regionale 28 giugno 2007 no. 19. Vendita diretta al consumatore di latte crudo vaccino, ovi-caprini e bufalini nell'azienda agricola di produzione – Modifiche e integrazioni alle Circolari n.39/SAN del 17 novembre 2004 e n.20/SAN del 24 maggio 2005″ – Precisazioni. BURL, Serie editoriale ordinaria 28 1944–1946Google Scholar
Reinemann, DJ, Mein, GA, Bray, DR, Reid, D & Britt, JS 1997 Troubleshooting high bacteria counts in farm milk. http://www.uwex.edu/MilkQuality/PDF/A3705.pdf. Accessed 15 November 2010Google Scholar
Reinemann, DJ, Wolters, GMVH, Billon, P, Lind, O & Rasmussen, MD 2000 Review of practices for cleaning and sanitation of milking machines. http://www.uwex.edu/uwmril/pdf/MilkMachine/Cleaning/00_Nagano_CIP.pdf. Accessed 15 November 2010Google Scholar
Ruegg, PL & Reinemann, DJ 2002 Milk quality and mastitis tests. Bovine Practitioner 3 41–54CrossRefGoogle Scholar
SAS 9.1, 2001 SAS Institute Inc., Cary NC, USAGoogle Scholar
Sørhaug, T & Stepaniak, L 1997 Psychrotrophs and their enzymes in milk and dairy products: Quality aspects. Trends in Food Science and Technology 8 35–41Google Scholar
Teixeira, P, Lopes, Z, Azeredo, J, Oliveira, R, & Vieira, MJ 2005 Physico-chemical surface characterization of a bacterial population isolated from a milking machine. Food Microbiology 22 247–251CrossRefGoogle Scholar
Villar, A, Garcia, JA, Iglesias, L, Garcia, ML & Otero, A 1996 Application of principal component analysis to the study of microbial populations in refrigerated raw milk from farms. International Dairy Journal 6 937–945CrossRefGoogle Scholar
Yin, Y & Ding, Y 2009 A close to real-time prediction method of total coliform bacteria in foods based on image identification technology and artificial neural network. Food Research International 42 191–199CrossRefGoogle Scholar