Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-27T14:09:54.742Z Has data issue: false hasContentIssue false
  • English
  • Français

Hormone variations in serum and milk of buffaloes (Bubalus bubalis) as potential indicators of treatment with recombinant bovine somatotropin

Published online by Cambridge University Press:  16 August 2011

Lorenzo Castigliego ,
Xiao Ning Li ,
Andrea Armani ,
Goffredo Grifoni ,
Carlo Boselli ,
Remo Rosati ,
Daniela Gianfaldoni  and
Alessandra Guidi
Lorenzo Castigliego*
Affiliation:
Department of Animal Pathology, Prophylaxis and Food Hygiene, University of Pisa, Via delle Piagge 2-56124, Pisa, Italy
Xiao Ning Li
Affiliation:
Department of Animal Science and Technology, University of Guangxi, Daxue Road 100, Nanning, People's Republic of China
Andrea Armani
Affiliation:
Department of Animal Pathology, Prophylaxis and Food Hygiene, University of Pisa, Via delle Piagge 2-56124, Pisa, Italy
Goffredo Grifoni
Affiliation:
Experimental Zooprophylactic Institute of Lazio and Tuscany, Via Appia Nuova 1411-00178, Rome, Italy
Carlo Boselli
Affiliation:
Experimental Zooprophylactic Institute of Lazio and Tuscany, Via Appia Nuova 1411-00178, Rome, Italy
Remo Rosati
Affiliation:
Experimental Zooprophylactic Institute of Lazio and Tuscany, Via Appia Nuova 1411-00178, Rome, Italy
Daniela Gianfaldoni
Affiliation:
Department of Animal Pathology, Prophylaxis and Food Hygiene, University of Pisa, Via delle Piagge 2-56124, Pisa, Italy
Alessandra Guidi
Affiliation:
Department of Animal Pathology, Prophylaxis and Food Hygiene, University of Pisa, Via delle Piagge 2-56124, Pisa, Italy
*
*For correspondence: lcastigl@vet.unipi.it
Get access Rights & Permissions [Opens in a new window]

Abstract

Recombinant bovine somatotropin (rbST) is used to increase milk yield in cows, but it has been forbidden in some countries and in the EU. However, rbST misuse represents a concern in both bovine and buffalo dairy production. A number of studies on rbST treatment have been performed on bovines, but there are few data on buffaloes. In this study, we treated eight lactating buffaloes with biweekly injections of a slow-release formulation of rbST, for five cycles of administration, and analysed total ST and insulin-like growth factor 1 (IGF-1) variations in serum and IGF-1 in milk. The aim was to assess their power as potential indicators of rbST-treatment. Blood was collected on days 2, 5, 9 and 14 of each cycle, and milk on days 2, 9 and 14 of cycles 2 and 5. Results showed an extraordinary increase in ST levels on day 2 in treated animals, followed by a rapid decrease over the following days, while a significant increase in IGF-1 was observed both in serum and in milk throughout most of each cycle. These results suggest that serum ST levels are a good indicator of treatment. However, the rapid decrease after the peak limits the useful period of sample collection.

Keywords

Recombinant bovine somatotropinbuffalomilkIGF-1
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 4 , November 2011 , pp. 412 - 420
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bauman, DE 1992 Bovine somatotropin: Review of an emerging animal technology. Journal of Dairy Science 75 3432–3451Google Scholar
Bilby, CR, Bader, JF, Salfen, BE, Youngquist, RS, Murphy, CN, Garverick, HA, Crooker, BA & Lucy, MC 1999 Plasma GH, IGF-I and conception rate in cattle treated with low doses of recombinant bovine GH. Theriogenology 51 1285–1296Google Scholar
Borghese, A 2005 Buffalo cheese and milk industry. Buffalo Production and Research, FAO X 185–196Google Scholar
Borghese, A & Mazzi, M 2005 Buffalo population and strategies in the world. Buffalo Production and Research, FAO I 1–39Google Scholar
Castigliego, L, Iannone, G, Grifoni, G, Rosati, R, Gianfaldoni, D & Guidi, A 2007 Natural and recombinant bovine somatotropin: immunodetection with a sandwich ELISA. Journal of Dairy Research 74 79–85Google Scholar
Castigliego, L, Grifoni, G, Rosati, R, Iannone, G, Armani, A, Gianfaldoni, D & Guidi, A 2009 On the alterations in serum concentration of somatotropin and insulin-like growth factor 1 in lactating cows after the treatment with a little studied recombinant bovine somatotropin. Research in Veterinary Science 87 29–35CrossRefGoogle ScholarPubMed
Castigliego, L, Li, X, Armani, A, Mazzi, M & Guidi, A 2011 An immunoenzymatic assay to measure insulin-like growth factor 1 (IGF-1) in buffalo milk with an IGF binding protein blocking pre-treatment of the sample. International Dairy Journal 21 421–426Google Scholar
Cisse, M, Chillard, Y, Coxam, V, Davicco, MJ & Remond, B 1991 Slow release somatotropin in dairy heifers and cows fed two levels of energy concentrate. 2. Plasma hormones and metabolites. Journal of Dairy Science 74 1382–1394CrossRefGoogle ScholarPubMed
Collier, RJ, Miller, MA, McLaughlin, CL, Johnson, HD & Baile, CA 2008 Effects of recombinant bovine somatotropin (rbST) and season on plasma and milk insulin-like growth factors I (IGF-I) and II (IGF-II) in lactating dairy cows. Domestic Animal Endocrinology 35 16–23CrossRefGoogle Scholar
Council Decision of 17 December 1999 concerning the placing on the market and administration of bovine somatotropin (BST). Official Journal of European Communities, 1999/879/ECGoogle Scholar
Cushman, RA, Desouza, JC, Hedgpeth, VS & Britt, JH 2001 Effect of long-term treatment with recombinant bovine somatotropin and estradiol on hormone concentrations and ovulatory response of superovulated cattle. Theriogenology 55 1533–1547CrossRefGoogle ScholarPubMed
Daxenberger, A, Breier, BH & Sauerwein, H 1998 Increased milk levels of insulin-like growth factor 1 (IGF-1) for the identification of bovine somatotropin (bST) treated cows. Analyst 123 2429–2435Google Scholar
Gallo, GF & Block, E 1990 Effects of recombinant bovine somatotropin on nutritional status and liver function of lactating dairy cows. Journal of Dairy Science 73 3276–3286CrossRefGoogle ScholarPubMed
Helal, FIS & Lasheen, MA 2008 The productive performance of Egyptian dairy buffaloes receiving biosynthetic bovine somatotropin (rbST) with or without monensin. American-Eurasian Journal of Agricultural & Environmental Science 3 771–777Google Scholar
Hodate, K, Ozawa, A & Johke, T 1991 Effect of a prolonged release formulation of recombinant bovine somatotropin on plasma concentrations of hormones and metabolites, and milk production in dairy cows. Endocrinology Japan 38 527–532CrossRefGoogle ScholarPubMed
Holzer, Z, Aharoni, Y, Brosh, A, Orlov, A, Veenhuizen, JJ & Kasser, TR 1999 The effects of long-term administration of recombinant bovine somatotropin (Posilac) and Synovex on performance, plasma hormone and amino acid concentration, and muscle and subcutaneous fat fatty acid composition in Holstein-Friesian bull calves. Journal of Animal Science 77 1422–1430CrossRefGoogle ScholarPubMed
Le Breton, MH, Rochereau-Roulet, S, Pinel, G, Cesbron, N & Le Bizec, B 2009 Elimination kinetic of recombinant somatotropin in bovine. Analytica Chimica Acta 637 121–127CrossRefGoogle ScholarPubMed
Ludri, RS, Upadhyay, RC & Singh, M 1989 Milk production in lactating buffalo receiving recombinantly produced bovine somatotropin. Journal of Dairy Science 72 2283–2287Google Scholar
McGuire, MA, Bauman, DE, Miller, MA & Hartnell, GF 1992 Response of somatomedins (IGF-I and IGF-II) in lactating cows to variations in dietary energy and protein and treatment with recombinant n-methionyl bovine somatotropin. Journal of Nutrition 122 128–136Google Scholar
Mielke, H, Lochmann, G, Simon, I, Bier, H, Jahreis, G, Hesse, V & Gallowitsch, F 1990 bSTH and IGF-I levels in blood and milk of black and white dairy cows before and after application of recombinantly derived bovine somatotropin. Zentralblatt für Veterinärmedizin Reihe A 37 554–557CrossRefGoogle ScholarPubMed
Peel, CJ, Fronk, TJ, Bauman, DE & Gorewit, RC 1983 Effect of exogenous growth hormone in early and late lactation on lactational performance of dairy cows. Journal of Dairy Science 66 776–782Google Scholar
Polidori, F, Sgoifo Rossi, CA, Senatore, EM, Savoini, G & Dell'Orto, V 1997 Effect of recombinant somatotropin and calcium salts of long chain fatty acids on milk from Italian buffalo. Journal of Dairy Science 80 2137–2142Google Scholar
Prosser, CG, Fleet, IR & Corps, AN 1989 Increased secretion of insulin-like growth factor I into milk of cows treated with recombinantly derived bovine growth hormone. Journal of Dairy Research 56 17–26Google Scholar
Putnam, DE, Varga, GA & Dann, HM 1999 Metabolic and production responses to dietary protein and exogenous somatotropin in late gestation dairy cows. Journal of Dairy Science 82 982–995Google Scholar
Schams, D, Graf, F, Meyer, J, Graule, B, Mauthner, M & Wollny, C 1991 Changes in hormones, metabolites, and milk after treatment with sometribove (recombinant methionyl bST) in Deutsches Fleckvieh and German black and white cows. Journal of Animal Science 69 1583–1592Google Scholar
Schwarz, FJ, Schams, D, Ropke, R, Kirchgessner, M, Kogel, J & Matzke, P 1993 Effects of somatotropin treatment on growth performance, carcass traits, and the endocrine system in finishing beef heifers. Journal of Animal Science 71 2721–2731Google Scholar
Slaba, J, Krejci, P, Skarda, J, Huybrechts, LM, Decuypere, E & Herrmann, H 1994 Plasma profiles of somatotropin and IGF-I in diary cows following application of two preparations of recombinant bovine somatotropin in a sustained release vehicle. Physiological Research 43 37–43Google Scholar
Spitsberg, VL 1987 A selective extraction of growth hormone from bovine pituitary gland and its further purification and crystallization. Analytical Biochemistry 160 489–495CrossRefGoogle ScholarPubMed
Zhao, X, McBride, BW, Trouten-Radford, LM, Golfman, L & Burton, JH 1994 Somatotropin and insulin-like growth factor-I concentrations in plasma and milk after daily or sustained-release exogenous somatotropin administrations. Domestic Animal Endocrinology 11 209–216Google Scholar