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Microscopic differential cell counts in milk for the evaluation of inflammatory reactions in clinically healthy and subclinically infected bovine mammary glands

Published online by Cambridge University Press:  16 August 2011

Daniel Schwarz ,
Ulrike S Diesterbeck ,
Sven König ,
Kerstin Brügemann ,
Karen Schlez ,
Michael Zschöck ,
Wilfried Wolter  and
Claus-Peter Czerny
Daniel Schwarz
Affiliation:
Department of Animal Sciences, Institute of Veterinary Medicine, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Sciences, Georg-August-University Göttingen, Burckhardtweg 2, D-37077 Göttingen, Germany
Ulrike S Diesterbeck
Affiliation:
Department of Animal Sciences, Institute of Veterinary Medicine, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Sciences, Georg-August-University Göttingen, Burckhardtweg 2, D-37077 Göttingen, Germany
Sven König
Affiliation:
Department of Animal Breeding, University of Kassel, Nordbahnhofstraße 1a, D-37213 Witzenhausen, Germany
Kerstin Brügemann
Affiliation:
Department of Animal Breeding, University of Kassel, Nordbahnhofstraße 1a, D-37213 Witzenhausen, Germany
Karen Schlez
Affiliation:
Landesbetrieb Hessisches Landeslabor, Schubertstraße 60, D-35392 Giessen, Germany
Michael Zschöck
Affiliation:
Landesbetrieb Hessisches Landeslabor, Schubertstraße 60, D-35392 Giessen, Germany
Wilfried Wolter
Affiliation:
Regierungspräsidium Giessen, Milk Control, Schanzenfeldstraße 8, D-35578 Wetzlar, Germany
Claus-Peter Czerny*
Affiliation:
Department of Animal Sciences, Institute of Veterinary Medicine, Division of Microbiology and Animal Hygiene, Faculty of Agricultural Sciences, Georg-August-University Göttingen, Burckhardtweg 2, D-37077 Göttingen, Germany
*
*For correspondence; e-mail: cczerny@gwdg.de
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Abstract

Somatic cell count (SCC) is generally regarded as an indicator of udder health. A cut-off value of 100×103 cells/ml is currently used in Germany to differentiate between normal and abnormal secretion of quarters. In addition to SCC, differential cell counts (DCC) can be applied for a more detailed analysis of the udder health status. The aim of this study was to differentiate somatic cells in foremilk samples of udder quarters classified as normal secreting by SCC <100×103 cells/ml. Twenty cows were selected and 72 normal secreting udder quarters were compared with a control group of six diseased quarters (SCC >100×103 cells/ml). In two severely diseased quarters of the control group (SCC of 967×103 cells/ml and 1824×103 cells/ml) Escherichia coli and Staphylococcus aureus were detected. DCC patterns of milk samples (n=25) with very low SCC values of ⩽6·25×103 cells/ml revealed high lymphocyte proportions of up to 92%. Milk cell populations in samples (n=41) with SCC values of (>6·25 to ⩽25)×103 cells/ml were also dominated by lymphocytes (mean value 47%), whereas DCC patterns of milk from udder quarters (n=6) with SCC values (>25 to ⩽100)×103 cells/ml changed. While in samples (n=3) with SCC values of (27–33)×103 cells/ml macrophages were predominant (35–40%), three milk samples with (43–45)×103 cells/ml indicated already inflammatory reactions based on the predominance of polymorphonuclear leucocytes (PMN) (54–63%). In milk samples of diseased quarters PMN were categorically found as dominant cell population with proportions of ⩾65%. Macrophages were the second predominant cell population in almost all samples tested in relationship to lymphocytes and PMN. To our knowledge, this is the first study evaluating cell populations in low SCC milk in detail. Udder quarters classified as normal secreting by SCC <100×103 cells/ml revealed already inflammatory processes based on DCC.

Keywords

Differential cell countssubclinical mastitisudder healthlow somatic cell counts
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 4 , November 2011 , pp. 448 - 455
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

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References

Coles, EH 1974 Leukocytes. In: Veterinary Clinical Pathology, 6th Edn, pp. 40–98. Philadelphia PA, USA: W B Saunders CompanyGoogle Scholar
Davis, AK, Maney, DL & Maerz, JC 2008 The use of leukocyte profiles to measure stress in vertebrates: a review for ecologists. Functional Ecology 22 760–772CrossRefGoogle Scholar
Dohoo, IR & Meek, AH 1982 Somatic cell counts in bovine milk. Canadian Veterinary Journal 23 119–125Google ScholarPubMed
Dosogne, H, Vangroenweghe, F, Mehrzad, J, Massart-Leën, AM & Burvenich, C 2003 Differential leukocyte count method for bovine low somatic cell count milk. Journal of Dairy Science 86 828–834Google Scholar
DVG (Deutsche Veterinärmedizinische Gesellschaft) 2000 [Guidelines for the collection of milk samples under antiseptic conditions.] Giessen, Germany: German Veterinary SocietyGoogle Scholar
DVG (Deutsche Veterinärmedizinische Gesellschaft) 2002 [Guidelines for the control of bovine mastitis as a herd problem.] Giessen, Germany: German Veterinary SocietyGoogle Scholar
Harmon, RJ 1994 Physiology of mastitis and factors affecting somatic cell counts. Journal of Dairy Science 77 2103–2112Google Scholar
Hill, AW, Shears, AL & Hibbitt, KG 1978 The elimination of serum-resistant Escherichia coli from experimentally infected single mammary glands of healthy cows. Research in Veterinary Science 85 89–93CrossRefGoogle Scholar
IDF, International Dairy Federation 1981 Laboratory methods for use in mastitis work. Document 132. Brussels, Belgium: IDFGoogle Scholar
Kehrli, ME & Shuster, DE 1994 Factors affecting milk somatic cells and their role in health of the bovine mammary gland. Journal of Dairy Science 77 619–627Google Scholar
Koess, C & Hamann, J 2008 Detection of mastitis in the bovine mammary gland by flow cytometry at early stages. Journal of Dairy Research 75 225–232Google Scholar
Lee, CS, FBP Wooding, P & Kemp, P 1980 Identification properties and differential counts of cell populations using electron microscopy of dry cows secretions, colostrum and milk from normal cows. Journal of Dairy Research 47 39–50Google Scholar
Leitner, G, Shoshani, E, Krifucks, O, Chaffer, M & Saran, A 2000 Milk leucocyte population patterns in bovine udder infection of different aetiology. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health 47 581–589Google Scholar
Leitner, G, Eligulashvily, R, Krifucks, O, Perl, S & Saran, A 2003 Immune cell differentiation in mammary gland tissues and milk of cows chronically infected with Staphylococcus aureus. Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health 50 45–52Google Scholar
Littell, RC, Henry, PR & Ammerman, CB 1998 Statistical analysis of repeated measures data using SAS procedures. Journal of Animal Science 76 1216–1231Google Scholar
Medzhitov, R 2007 Recognition of microorganisms and activation of the immune response. Nature 449 819–826Google Scholar
Merle, R, Schröder, AC & Hamann, J 2007 Cell function in the bovine mammary gland: a preliminary study on interdependence of healthy and infected udder quarters. Journal of Dairy Research 74 174–179Google Scholar
Miller, RH, Paape, MJ & Fulton, LA 1991 Variation in milk somatic cells of heifers at first calving. Journal of Dairy Science 74 3782–3790Google Scholar
Newbould, FHS & Neave, FK 1965 The recovery of small numbers of Staphylococcus aureus infused into the bovine teat cistern. Journal of Dairy Research 32 157–162Google Scholar
Nickerson, SC 1989 Immunological aspects of mammary involution. Journal of Dairy Science 72 1665–1678Google Scholar
Olde Riekerink, RGM, Barkema, HW, Veenstra, W, Berg, FE, Stryhn, H & Zadoks, RN 2007 Somatic cell counts during and between milkings. Journal of Dairy Science 90 3733–3741Google Scholar
Oviedo-Boyso, J, Valdez-Alarcón, JJ, Cajero-Juárez, M, Ochoa-Zarzosa, A, López-Meza, JE, Bravo-Patiño, A & Baizabal-Aguirre, VM 2007 Innate immune response of bovine mammary gland to pathogenic bacteria responsible for mastitis. Journal of Infection 54 399–409CrossRefGoogle ScholarPubMed
Paape, MJ, Wergin, WP, Guidry, AJ & Pearson, RE 1979 Leukocytes—Second line of defense against invading mastitis pathogens. Journal of Dairy Science 62 135–153Google Scholar
Paape, MJ, Mehrzad, J, Zhao, X, Detilleux, J & Burvenich, C 2002 Defense of the bovine mammary gland by polymorphonuclear neutrophil leukocytes. Journal of Mammary Gland Biology and Neoplasia 7 109–121Google Scholar
Paape, MJ, Bannerman, DD, Zhao, X & Lee, J-W 2003 The bovine neutrophil: structure and function in blood and milk. Veterinary Research 34 597–627Google Scholar
Pappenheim, A 1912 [Staining of blood cells in clinical dried-blood preparation of hematopoietic tissue according to my method.]. Folia Haematologica: Internationales Magazin für klinische und morphologische Blutforschung 13 337–344Google Scholar
Piccinini, R, Bronzo, V, Moroni, P, Luzzago, C & Zecconi, A 1999 Study on the relationship between milk immune factors and Staphylococcus aureus intramammary infections in dairy cows. Journal of Dairy Research 66 501–510Google Scholar
Pillai, SR, Kunze, E, Sordillo, LM & Jayarao, BM 2001 Application of differential inflammatory cell count as a tool to monitor udder health. Journal of Dairy Science 84 1413–1420Google Scholar
Reiter, B 1978 Review of progress of dairy science—Anti-microbial systems in milk. Journal of Dairy Research 45 131–147Google Scholar
Rivas, AL, Quimby, FW, Blue, J & Coksaygan, O 2001 Longitudinal evaluation of bovine mammary gland health status by somatic cell counting, flow cytometry, and cytology. Journal of Veterinary Diagnostic Investigation 13 399–407CrossRefGoogle ScholarPubMed
Sarikaya, H, Werner-Misof, C, Atzkern, M & Bruckmaier, RM 2005 Distribution of leucocyte populations, and milk composition, in milk fractions of healthy quarters in dairy cows. Journal of Dairy Research 72 486–492CrossRefGoogle ScholarPubMed
Sarikaya, H & Bruckmaier, RM 2006 Importance of the sampled milk fraction for the prediction of total quarter somatic cell count. Journal of Dairy Science 89 4246–4250Google Scholar
Schröder, AC & Hamann, J 2005 The influence of technical factors on differential cell count in milk. Journal of Dairy Research 72 153–158CrossRefGoogle ScholarPubMed
Schwarz, D, Diesterbeck, US, Failing, K, König, S, Brügemann, K, Zschöck, M, Wolter, W & Czerny, C-P 2010 Somatic cell counts and bacteriological status in quarter foremilk samples of cows in Hesse, Germany—A longitudinal study. Journal of Dairy Science 93 5716–5728Google Scholar
Sears, PM, Smith, BS, English, PB, Herer, PS & Gonzalez, RN 1990 Shedding pattern of Staphylococcus aureus from bovine intramammary infections. Journal of Dairy Science 73 2785–2789Google Scholar
Sordillo, LM & Nickerson, SC 1988 Morphometric changes in the bovine mammary gland during involution and lactogenesis. American Journal of Veterinary Research 49 1112–1120Google Scholar
Sordillo, LM, Shafer-Weaver, K & DeRosa, D 1997 Immunobiology of the mammary gland. Journal of Dairy Science 80 1851–1865Google Scholar
Targowski, SP 1983 Role of immune factors in protection of mammary gland. Journal of Dairy Science 66 1781–1789Google Scholar
Vangroenweghe, F, Dosogne, H, Mehrzad, J & Burvenich, C 2001 Effect of milk sampling techniques on milk composition, bacterial contamination, viability and functions of resident cells in milk. Veterinary Research 32 565–579Google Scholar
Viguier, C, Arora, S, Gilmartin, N, Welbeck, K & O'Kennedy, R 2009 Mastitis detection: current trends and future perspectives. Trends in Biotechnology 27 486–493CrossRefGoogle ScholarPubMed
Wever, PU & Emanuelson, U 1989 Effects of systematic influences and intramammary infection on differential and total somatic cell counts in quarter milk samples of dairy cows. Acta Veterinaria Scandinavica 30 465–474CrossRefGoogle ScholarPubMed