Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-13T04:25:35.223Z Has data issue: false hasContentIssue false
  • English
  • Français

Milk fatty acid characterization and genetic parameter estimates for milk conjugated linoleic acid in buffaloes

Published online by Cambridge University Press:  04 March 2011

Humberto Tonhati ,
André LF Lima ,
Dante PD Lanna ,
Gregório MF de Camargo ,
Fernando Baldi ,
Lucia G de Albuquerque  and
Jeanne MCD Montrezor
Humberto Tonhati*
Affiliation:
Animal Science Department, São Paulo State University (FCAV/Unesp), Jaboticabal, SP, Brazil Conselho Nacional de Desenvolvimento Científico e Tecnologico (CNPq) and Instituto Nacional de Ciência e Tecnologia - Ciência Animal (INCT- CA), Viçosa, MG, Brazil
André LF Lima
Affiliation:
Animal Science and Rural Development Department, Santa Catarina Federal University, Florianópolis, SC, Brazil
Dante PD Lanna
Affiliation:
Animal Science Department, São Paulo University (ESALQ/USP), Piracicaba, SP, Brazil
Gregório MF de Camargo
Affiliation:
Animal Science Department, São Paulo State University (FCAV/Unesp), Jaboticabal, SP, Brazil
Fernando Baldi
Affiliation:
Animal Science Department, São Paulo State University (FCAV/Unesp), Jaboticabal, SP, Brazil
Lucia G de Albuquerque
Affiliation:
Animal Science Department, São Paulo State University (FCAV/Unesp), Jaboticabal, SP, Brazil Conselho Nacional de Desenvolvimento Científico e Tecnologico (CNPq) and Instituto Nacional de Ciência e Tecnologia - Ciência Animal (INCT- CA), Viçosa, MG, Brazil
Jeanne MCD Montrezor
Affiliation:
Animal Science Department, São Paulo State University (FCAV/Unesp), Jaboticabal, SP, Brazil
*
*For correspondence; tonhati@fcav.unesp.br
Get access Rights & Permissions [Opens in a new window]

Abstract

The objectives of this study were to analyse buffalo milk fat composition, to verify the activity of Delta(9)-desaturase enzyme in the mammary gland, as well as to estimate additive genetic variances for milk, fat and protein yield, and milk cis-9,trans-11 conjugated linoleic acid percentage (cis-9,trans-11 CLA%). A total of 3929 lactation milk yields (MY) records from 2130 buffaloes and 1598 lactation fat (FY) and protein (PY) yield records from 914 buffaloes were analysed. For cis-9,trans-11 CLA%percentage, a total of 661 milk samples from 225 buffaloes, daughters of 8 sires, belonging to 4 herds and calving in 2003 and 2004, were used. The genetic parameters and variance components were estimated by Restricted Maximum Likelihood applying an animal model. The fixed effects considered in the model were: contemporary group (herd, year, calving season) and age at calving (linear and quadratic effects) and lactation length (linear and quadratic effects) as covariables. Additive genetic and permanent environment effects were considered as random. The MY, FY, PY and CLA% means were 1482±355 kg, 90·1±24·6 kg, 56·9±15·2 kg and 0·69±0·16%, respectively. Heritability estimates for MY, FY, PY and CLA% were 0·28±0·05, 0·26±0·11, 0·25±0·11 and 0·35±0·14, respectively. There is enough additive genetic variation for buffalo milk, protein and fat yield to improve these traits through selection. The cis-9,trans-11 CLA% can be enhanced by selection in buffaloes and will contribute to improving human health. The activity and efficiency of Delta(9)-desaturase in the mammary was measured and confirmed.

Keywords

Bubalus bubalisCLAheritabilitymilk fatty acids profileDelta(9)-desaturase activity
Type
Research Article
Information
Journal of Dairy Research , Volume 78 , Issue 2 , May 2011 , pp. 178 - 183
Copyright
Copyright © Proprietors of Journal of Dairy Research 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aspilcueta-Borquis, RR, Sesana, RC, Muñoz-Berrocal, MH, Seno, LO, Bignardi, AB, El Faro, L, Albuquerque, LG, de Camargo, GMF & Tonhati, H 2010a Genetic parameters for milk, fat and protein yields in Murrah buffaloes (Bubalus bubalis Artiodactyla, Bovidae). Genetics and Molecular Biology 33 71–77CrossRefGoogle ScholarPubMed
Aspilcueta-Borquis, RR, Di Palo, R, Araújo Neto, FR, Baldi, F, de Camargo, GMF, Albuquerque, LG, Zicarelli, L & Tonhati, H 2010b Genetic parameter estimates for buffalo milk yield, milk quality and mozzarella production and Bayesian inference analysis of their relationships. Genetics and Molecular Research 9 1636–1644CrossRefGoogle ScholarPubMed
Aspilcueta-Borquis, RR, Araújo Neto, FR, Baldi, F, Bignardi, AB, Albuquerque, LG & Tonhati, H 2010c Genetic parameters for buffalo milk yield and milk quality traits using Bayesian inference. Journal of Dairy Science 93 2195–2201CrossRefGoogle ScholarPubMed
Boldman, KG, Kriese, LA, Van Vleck, LD & Kachman, SD 1995 A Manual for use MTDFREML. Department of Agriculture/Agricultural Research Service, Lincoln NE, USA 120 pp.Google Scholar
Carta, A, Piredda, G, Addis, M, Cabiddu, A, Fiori, M, Leroux, C & Barillet, F 2003 Fatty acid composition of sheep milk from a backcross Sarda×Lacaune resource population: Preliminary QTL detection for CLA content. In: Breeding Programmes for Improving the Quality and Safety of Products. New Traits, Tools, Rules and Organization? (Eds Gabiña, D & Sanna, S) pp. 107–113. Zaragoza, Spain: CIHEAM-IAMZGoogle Scholar
Cesano, A, Visonneau, S, Scimeca, JA, Kritchevsky, D & Santoli, D 1998 Opposite effects of linoleic acid and conjugated linoleic acid on human prostatic cancer in SCID mice. Anticancer Research 18 1429–1434Google ScholarPubMed
Chouinard, PY, Corneau, L, Barbano, DM, Metzger, LE & Bauman, DE 1999 Conjugated linoleic acids alter milk fatty acid composition and inhibit milk fat secretion in dairy cows. Journal of Nutrition 129 1579–1584CrossRefGoogle ScholarPubMed
Corl, BA, Lacy, SH, Baumgard, LH, Dwyer, DA, Griinari, JM, Phillips, S & Bauman, DE 1999 Examination of the importance of Δ9-dessaturase and endogenous synthesis of conjugated linoleic acid in lactating dairy cows. Animal Science 77 118Google Scholar
De Camargo, GMF, Lima, ALF, Thomazine, RB, Baldi, F, Albuquerque, LG & Tonhati, H 2010 Stearoyl-CoA-Desaturase's promoter region of buffaloes and its correlation with conjugated linoleic acid in milk. 26th World Buiatrics Congress, Santiago, ChileGoogle Scholar
Fedele, E, Iannibeci, L, Marzillo, G, Ferrara, L & Bergamo, P 2001 Conjugated linoleic acid content in milk and mozzarela cheese from buffalo fed with organic and traditional diet. In: World Buffalo Congress, 6. Maracaibo, Proceedings. Maracaibo: Zulia University. Technology Park, 404–409Google Scholar
Feng, S, Salter, AM, Parr, T & Garnsworthy, PC 2007 Extraction and quantitative analysis of stearoyl-coenzyme A desaturase mRNA from dairy cow milk somatic cells. Journal of Dairy Science 90 4128–4136CrossRefGoogle ScholarPubMed
Fernandes, SAA, Mattos, WRS, Matarazzo, SV, Tonhati, H, Sundfeld Gama, MA & Lanna, DPD 2007 Activity of Δ9-desaturase enzyme in mammary gland of lactating buffaloes. Italian Journal of Animal Science 6 1060–1062CrossRefGoogle Scholar
Fievez, V, Vlaeminck, B, Dhanoa, MS & Dewhurst, RJ 2003 Use of principal components analysis to investigate the origin of heptadecenoic acid and conjugated linoleic acids in milk. Journal of Dairy Science 86 1017–1053CrossRefGoogle ScholarPubMed
Gavino, VC, Gavino, G, Leblanc, MJ & Tuchweber, B 2000 An isomeric mixture of conjugated linoleic acids but not pure cis-9,trans-11-octadecadienoic acid affects body weight and plasma lipids in hamsters. Journal Nutrition 130 27–29CrossRefGoogle Scholar
Garnsworthy, PC, Feng, S, Lock, AL & Royal, MD 2010 Heritability of milk fatty acid composition and stearoyl-CoA desaturase indices in dairy cows. Journal of Dairy Science 93 1743–1748Google Scholar
Griinari, JM & Bauman, DE 1999 Biosynthesis of conjugated linoleic acid and its incorporation into meat and milk in ruminants. In: Advances in Conjugated Linoleic acid Research Vol 1 (Eds Yurawecz, MP, Mossoba, MM, Kramer, JKG et al. ) pp. 180–200. Champaign IL, USA: American Oil Chemists Society PressGoogle Scholar
Griinari, JM, Corl, BA, Lacy, SH, Chouinard, PY, Nurmela, KVV & Bauman, DE 2000 Conjugated linoleic acid is synthesized endogenously in lactating dairy cows by Delta(9)-desaturase. Journal of Nutrition 130 2285–2291Google Scholar
Ha, YL, Grimm, NK & Pariza, MW 1987 Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis 8 1881–1887CrossRefGoogle ScholarPubMed
Ha, YL, Storkson, J & Pariza, MW 1990 Inhibition of benzo(a)pyrene-induced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid. Cancer Research 50 1097–1101Google ScholarPubMed
Hara, A & Radim, NS 1978 Lipid extraction of tissues with low toxicity solvent. Analytical Biochemistry 90 420–426CrossRefGoogle ScholarPubMed
Hurtado-Lugo, N, Cerón-Muñoz, M & Gutiérrez-Valencia, A 2006 [Estimation of genetic parameters for milk production on control days in buffaloes in Costa Atlántica Colombia]. Livestock Research for Rural Development 18 3 www.cipav.org.co/lrrd/lrrd18/3/hurt18039.htmGoogle Scholar
Ip, C, Singh, M, Thompson, HJ & Scimeca, JA 1994 Conjugated linoleic acid suppresses mammary carcinogenesis and proliferative activity of the mammary gland in the rat. Cancer Research 54 1212–1215Google ScholarPubMed
Ip, C, Banni, S, Angioni, E, Carta, G, McGinley, J, Thompson, HJ, Barbano, D & Bauman, DE 1999 Conjugated linoleic acid-enriched butter fat alters mammary gland morphogenesis and reduces cancer risk in rats. Journal of Nutrition 129 2135–2142Google Scholar
Jensen, RG 2002 The composition of bovine milk lipids: January 1995 to December 2000. Journal of Dairy Science 85 295–350Google Scholar
Kelly, ML, Berry, JR, Dwyer, DA, Griinari, JM, Chouinard, PY, Van Amburgh, ME & Bauman, DE 1998 Dietary fatty acid sources affect conjugated linoleic acid concentrations in milk from lactating dairy cows. Journal of Nutrition 128 881–885CrossRefGoogle ScholarPubMed
Lawless, F, Murphy, JJ, Harrington, D, Devery, R & Stanton, C 1998 Elevation of conjugated cis-9, trans-11-octadecadiencoic acid in bovine milk because of dietary supplementation. Journal Animal Science 81 3259–3267Google ScholarPubMed
Lima, ALF, Otaviano, AR, Laureano, MMM, Galeazzi, PM, de Camargo, GMF, Thomazine, RB & Tonhati, H 2007 Partial genetic characterization of Stearoyl CoA-Desaturase´s structural region in Bubalus bubalis. Italian Journal of Animal Science 6 287–290CrossRefGoogle Scholar
Lee, KN, Kritchevsky, D & Pariza, MW 1994 Conjugated linoleic acid an atherosclerosis in rabbits. Atherosclerosis 108 19–25CrossRefGoogle ScholarPubMed
Lock, AL & Garnsworthy, PC 2003 Seasonal variation in milk conjugated linoleic acid and Delta(9)-desaturase activity in dairy cows. Livestock Production Science 79 47–59Google Scholar
Lock, AL & Bauman, DE 2004 Modifying milk fat composition of dairy cows to enhance fatty acids beneficial to human health. Lipids 39 1197–1206CrossRefGoogle ScholarPubMed
Maijala, K 2000 Cow milk and human development and well-being. Livestock Production Science 65 1–18Google Scholar
Mele, M, Dal Zotto, R, Cassandro, M, Conte, G, Serra, A, Buccioni, A, Bittante, G & Secchiari, P 2009 Genetic parameters for conjugated linoleic acid, selected milk fatty acids, and milk fatty acid unsaturation of Italian Holstein-Friesian cows. Journal of Dairy Science 92 392–400Google Scholar
Melício, SPL, Carvalho, MRBC, Tonhati, H, Munari, DP, Holgado, APR, Larosa, G & Aiura, FS 2005 [Chemical composition of milk of Murrah buffaloes in the São Carlos region]. Revista do Instituto de Laticínios Cândido Tostes 60 346–347Google Scholar
Nestel, PJ 1987 Polyunsaturated fatty acids (n-3, n-6). American Journal of Clinical Nutrition 45 1161–1167CrossRefGoogle ScholarPubMed
Nestel, P, Fujii, A & Allen, T 2006 The cis-9,trans-11 isomer of conjugated linoleic acid (CLA) lowers plasma triglyceride and raises HDL cholesterol concentrations but does not suppress aortic atherosclerosis in diabetic apoE-deficient mice. Atherosclerosis 189 282–287CrossRefGoogle Scholar
Palmquist, DL, Beaulieu, AD & Barbano, DM 1993 Feed and animal factors influencing milk fat composition. Journal of Dairy Science 76 1753–1771CrossRefGoogle ScholarPubMed
Pariza, MW, Park, Y & Cook, ME 2000 Mechanisms of action of conjugated linoleic acid: evidence and speculation. Proceedings of the Society for Experimental Biology and Medicine 223 8–13Google ScholarPubMed
Rosati, A & Van Vleck, LD 2002 Estimation of genetic parameters for milk, fat, protein and mozzarella cheese production in Italian river buffalo population. Livestock Production Science 74 185–190CrossRefGoogle Scholar
Shultz, TD, Chew, BP, Seaman, WR & Luedecke, LO 1992 Inhibitory effect of conjugated dienoic derivatives of linoleic acid and β-carotene on the in vitro growth of human cancer cells. Cancer Letters 63 125–133CrossRefGoogle ScholarPubMed
Stoop, WM, van Arendonk, JAM, Heck, JML, van Valenberg, HJF & Bovenhuis, H 2008 Genetic parameters for major milk fatty acids and milk production traits of Dutch Holstein-Friesians. Journal of Dairy Science 91 385–394CrossRefGoogle ScholarPubMed
Tonhati, H, Cerón-Muñoz, M, Oliveira, JA, Duarte, JMC, Furtado, TP & Tseimazides, SP 2000 [Genetic parameters for the production of milk, fat and protein in buffaloes]. Brazilian Journal of Animal Science 29 2051–2056Google Scholar
Van Nieuwenhove, C, Gauffin, P, Pérez, AC & Silvia, G 2007 Chemical composition and fatty acid content of Buffalo cheese from northwest Argentina: effect on lipid composition of mice tissues. Journal of Food Lipids 14 232–243Google Scholar
Visonneau, S, Cesano, A, Tepper, SA, Scimeca, JA, Santoli, D & Kritchevsky, D 1997 Conjugated linoleic acid suppresses the growth of human breast adenocarcinoma cells in SCID mice. Anticancer Research 17 969–974Google ScholarPubMed
White, SL, Bertrand, JA, Wade, MR, Washburn, SP, Green, JT & Jenkins, TC 2001 Comparison of fatty acid content of milk from Jersey and Holstein cows consuming pasture or a total mixed ration. Journal of Dairy Science 84 2295–2301CrossRefGoogle ScholarPubMed