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Radioimmunoassay for measurement of bovine α-lactalbumin in serum, milk and tissue culture media

Published online by Cambridge University Press:  01 June 2009

R. Michael Akers ,
Thomas B. McFadden ,
W. E. Beal ,
Albert J. Guidry  and
Harold M. Farrell
R. Michael Akers
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Thomas B. McFadden
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
W. E. Beal
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Albert J. Guidry
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
Harold M. Farrell
Affiliation:
Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA
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Summary

A sensitive, specific radioimmunoassay for bovine α-lactalbumin (α-la) suitable for measurement of serum, tissue culture media and milk was developed. α-La was not detected in serum from prepubertal male or female cattle, but was detected as early as 60 d of gestation in nulliparous Holstein heifers, the level being greatly increased during the last one third of gestation. Serum from cross bred beef heifers contained less α-la and it was not detected until late gestation. Concentrations of α-la in serum samples from pregnant multiparous Holstein cows decreased at drying-off and subsequently increased just before parturition. Secretion of α-la by mammary tissue explants from steroid-primed prepubertal Holstein heifers was induced by the addition of bovine prolactin, ovine prolactin or human growth hormone to tissue culture media.

Type
Original Articles
Information
Journal of Dairy Research , Volume 53 , Issue 3 , August 1986 , pp. 419 - 429
Copyright
Copyright © Proprietors of Journal of Dairy Research 1986

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References

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