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Effect of niclosamide on the tegumental surface of Haplorchis taichui using scanning electron microscopy

Published online by Cambridge University Press:  01 December 2007

K. Kumchoo ,
C. Wongsawad ,
P. Vanittanakom ,
J.Y. Chai  and
A. Rojanapaibul
K. Kumchoo*
Affiliation:
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand: Faculty of Arts and Sciences, Prince of Songkla University, Surat Thani Campus, Surat Thani 84100, Thailand:
C. Wongsawad
Affiliation:
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand:
P. Vanittanakom
Affiliation:
Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand:
J.Y. Chai
Affiliation:
Department of Parasitology and Institute of Endemic Diseases, College of Medicine Seoul National University, Seoul 110-799, Korea
A. Rojanapaibul
Affiliation:
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand:
*
*E-mail: kan_kumchoo@yahoo.com
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Abstract

The effect of niclosamide on the tegument of adult Haplorchis taichui (Trematoda: Heterophyidae) exposed in vitro was observed by scanning electron microscope. Adult worms were incubated in Tyrode's solution containing 0.01, 0.1, 1.0, and 10 μg ml− 1 of niclosamide for 30 min, 1, 6, 12 and 24 h. Control groups were incubated in Tyrode's solution without niclosamide and worms remained active until 24 h. In 0.01 μg ml− 1 of niclosamide, worms showed slightly active movements up to 1 h after incubation, while in 0.1 μg ml− 1 solution a few worms showed only slightly active movements after 30 min. Tegumental changes were determined by scanning electron microscopy. Swelling and blebbing of the tegument were observed on both ventral and dorsal sides. After longer periods, extensive swelling and blebbing of the tegument became more severe and there was a loss of the apical plasma membrane in some regions. Empty spine sockets occurred, and small perforations penetrated the basal lamina, followed by some lesions. Destruction of both surfaces was more pronounced on the posterior compared with the anterior regions.

Type
Research Papers
Information
Journal of Helminthology , Volume 81 , Issue 4 , December 2007 , pp. 329 - 337
Copyright
Copyright © Cambridge University Press 2007

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