We investigated trophic resource partitioning in seven syntopic anurans from low- and mid-elevation stream habitats of a tropical riparian ecosystem by utilising stomach content analysis (SCA) and stable isotope analysis (SIA). Our SCA data revealed dietary similarities, narrow trophic niche breadth, and low dietary niche overlap in Ansonia muelleri, Limnonectes magnus, Occidozyga laevis, Megophrys stejnegeri, Pulchrana grandocula, Sanguirana mearnsi, and Staurois natator which could be attributed to these anurans’ selection of available local prey items. We confirmed ant-specialisation (myrmecophagy) of the Mindanao island endemic bufonid A. muelleri based on our temporal SCA dietary data. Our SIA estimates of assimilation of potential prey sources confirmed that L. magnus, P. grandocula, and O. laevis are generalist predators, opportunistically feeding on locally abundant insect prey items. This study on trophic resource partitioning in syntopic anurans provides the first picture of trophic interactions, i.e., predation and competition in stream communities in tropical riparian zones of a watershed ecosystem in northeast Mindanao of the southern Philippines.

It is well concluded that microbial composition and diversity of coral species can be affected under temperature alterations. However, the interaction of environmental accumulation of corals and temperature stress on symbiotic Symbiodiniaceae and bacterial communities are rarely studied. In this study, two groups of soft coral Sarcophyton trocheliophorum were cultured under constant (26 °C) and inconstant (22 °C to 26 °C) temperature conditions for 30 days as control treatments. After that, water was cooled rapidly to decrease to 20 °C in 24 h. The results of diversity analysis showed that symbiotic Symbiodiniaceae and bacterial communities had a significant difference between the two accumulated groups. The principal coordinate analyses confirmed that symbiotic Symbiodiniaceae and bacterial communities of both control treatments were clustered into two groups. Our results evidenced that rapid cooling stress could not change symbiotic Symbiodiniaceae and bacterial communities’ composition. On the other hand, cooling stress could alter only bacterial communities in constant group. In conclusion, our study represents a clear relationship between environmental accumulation and the impact of short-term cooling stress in which microbial composition structure can be affected by early adaptation conditions.

Myrmecochory, the dispersal of seeds with lipid-rich appendages by ants, is a significant ant–plant interaction. Less well understood is the potential for ant dispersal of non-myrmecochorous seeds. Here we investigate ant–diaspore interactions in a West African montane habitat. We combine observation with depot experiments to determine ant species that move diaspores and distance moved across a forest-edge-grassland gradient. We recorded seed cleaning by ants using a bird/mammal dispersed Paullinia pinnata to determine whether seed cleaning improved plant fitness. We found that two out of a total of 17 ant species (Pheidole sp. 1 and Myrmicaria opaciventris) interacted with 10 species of non-myrmecochorous diaspores across nine plant families. Diaspores were from large canopy trees, understorey trees and vines. Both ant species interacted with small (≤0.24 g) and large (≥0.24 g) diaspores. Ants individually moved small diaspores up to 1.2 m and worked together to clean larger ones. Our experiments with P. pinnata showed that ants removed the pulp of 70% of fruit over 5 days. Cleaned seeds germinated significantly faster and produced seedlings with significantly longer shoot length and higher fresh weight than seedlings from intact seeds. Together our results suggest that ant dispersal may be less significant than seed cleaning in Afromontane forests. However, given the decline in vertebrate frugivores across Africa, a small dispersal advantage may become increasingly important to plant fitness.

Roller dung beetles play a pivotal role in the nutrient distribution in soil and secondary dispersal of seeds. Dung beetles are sampled either using a dung-baited pitfall trap or an exposed dung pile on the ground. While the former method is useful for a rapid survey of dung beetles, information on the ecology and behaviour of dung beetles can be lost, which the latter method provides, but underestimates species diversity due to its inefficiency in trapping rollers. Efficiency of a new method for sampling rollers—installing guarding pitfall traps around dung piles—is assessed in three habitats—contiguous tropical rainforests, fragmented forests, and disturbed used home gardens—and two diel periods—day and night. Five guarding pitfall traps were installed at 50 cm radius around dung piles. About 98% of the total rollers were sampled in pitfall traps. The habitats were similar when the roller catches of only dung piles—conventional approach—were analyzed, but were different when the rollers of guarding pitfall traps were considered. The roller abundance was negatively affected by forest fragmentation and land-use change. About 98% of the rollers were collected at daytime. Using guarding pitfall traps around dung piles is highly recommended for dung beetle diversity studies.

We used a standardised transect method to compare lowland forest termite assemblages in Buton Island, Sulawesi, with transects in Sundaland. The four Buton transects were extremely depauperate with species density ranging from 1 to 6 species, which is around 10% of the species density in 11 described Sundaland transects. Soil-feeding species were absent from the Buton transects but represent some 43% of species in the Sundaland transects. The Buton transects have relatively high soil pH (6.7–7.9), which may be associated with depauperate termite assemblages. Most termite genera recorded in Sulawesi are wood nesters that can raft in floating wood, which is probably how they arrived in Sulawesi. The Macrotermitinae (fungus-growers) do not raft and probably flew across serendipitously. Geographic isolation, both on Buton and in Sulawesi more generally, and Buton’s underlying geology causing high soil pH, may account for the near-absence of soil-nesters and soil-feeders, none of which are known to raft.

We examined the floristic composition and stand structure of tropical mountain forests containing two pine species, Pinus krempfii Lecomte and Pinus dalatensis Ferré, on the Da Lat Plateau in southern Vietnam. A total of 92 tree species were identified, and the greatest species richness at the family level was found in Lauraceae and Fagaceae. Both pine species grew to more than 25 m in height and spread their crowns over the continuous canopy layer. Under crowns of P. krempfii, Castanopsis chinensis, Trigonobalanus verticillata, Engelhardia roxburghiana, and Dendropanax hainanensis constituted the continuous canopy layer. Under crowns of P. dalatensis, pioneer species such as Schima wallichii, Exbucklandia populnea, and Pentaphylax euryoides along with gymnosperms such as Dacrycarpus imbricatus and Dacrydium elatum constituted the continuous canopy layer. Juveniles of P. krempfii were prevalent on the forest floor, but juveniles of P. dalatensis were scarce. We suggest that two pine species have different regeneration requirements related to disturbance and soil condition.

Fuchsia parviflora is a dioecious shrub that depends on biotic pollination for reproduction. Previous studies suggest that the male plants produce more flowers, and male-biased sex ratios have been found in some natural populations. To assess whether the biased sex ratios found between genders in natural populations are present at the point at which plants reach sexual maturity, and to identify possible trade-offs between growth and reproduction, we performed a common garden experiment. Finally, to complement the information of the common garden experiment, we estimated the reproductive biomass allocation between genders in one natural population. Sex ratios at reaching sexual maturity in F. parviflora did not differ from 0.5, except in one population, which was the smallest seedling population. We found no differences between genders in terms of the probability of germination or flowering. When flowering began, female plants were taller than males and the tallest plants of both genders required more time to reach sexual maturity. Males produced significantly more flowers than females, and the number of flowers increased with plant height in both genders. Finally, in the natural population studied, the investment in reproductive biomass was seven-fold greater in female plants than in male plants. Our results showed no evidence of possible trade-offs between growth and reproduction. Despite the fact that female plants invest more in reproductive biomass, they were taller than the males after flowering, possibly at the expense of herbivory defence.

Landscape-scale habitat loss can change the floristic composition of forest fragments, affecting the survival of specific groups of plants, as shade-tolerant and emergent trees. This increasing in tree mortality creates forest canopy gaps of different sizes that ultimately determine the solar radiation available in the forest understorey. We conducted a study aiming to assess how the loss of forest cover at landscape level (i.e. deforestation) affects the sunfleck dynamics, a proxy of light regime in forest understorey. We expected that fragments located in landscapes with less forest cover have a high number of larger canopy gaps and, consequently, long-lasting sunflecks. In each forest fragment, a 100 per 50 m plot was established, and in each plot, we took 10 hemispherical photographs. The images were analysed using the Gap Light Analyzer software. The sunflecks were divided into six temporal classes. We evidenced that landscape-scale deforestation increased the frequency of all sunfleck intervals >8 min, particularly the long-lasting (> 32 min) sunflecks. We propose that the increasing frequency of long-lasting sunflecks reduces suitability of microhabitat to some shade-tolerant species in local fragments, a potential proximal mechanism contributing to compositional shifts of tree assemblages observed in forest fragments within deforested landscapes.

Paramos are high-elevation tropical Andean ecosystems above the tree line that display variable temperature and frequent freezing spells. Because a significant anuran community lives in this environment, physiological protection against freezing must characterise individuals in this community. Antifreeze protection has been studied in amphibians from other communities, and it is likely that Paramo anurans rely on the same underlying molecules that convey such protection to Nearctic species. However, given the pervasive presence of freezing spells in the Paramos year-round, the processes of activating protection mechanisms may differ from that of seasonal counterparts. Accordingly, this study investigated cryoprotection strategies in high-elevation tropical frogs, using as a model the terrestrial and nocturnal genus Pristimantis, specifically P. bogotensis, P. elegans and P. nervicus from Paramos, and the warm ecosystem counterparts P. insignitus, P. megalops and P. sanctaemartae. We focused on freeze tolerance and its relationship with glucose accumulation and ice formation. Under field conditions, the highest elevation P. nervicus exhibited higher glucose concentration at dawn compared to noon (1.7 ± 0.6 mmol/L versus 3.5 ± 1.32 mmol/L). Under experimental thermal freeze exposure for 2 hours between −2 and −4 ºC, the glucose concentration of the three Paramo species increased but physiological diversity was evident (P. nervicus 126%; P. bogotensis 100%; and P. elegans 55%). During this test, body ice formation was assessed calorimetrically. The species with the highest body ice formation was P. bogotensis (17% ± 5.37; maximum value: 63%; n = 8), followed by P. nervicus (5% ± 3.27; maximum value: 11%; n = 5) and P. elegans (0.34% ± 0.09; maximum value: 1%; n = 4). The study shows physiological diversity both within a genus and across the amphibian community around the freezing contour. Overall, Paramo species differ in freezing physiology from their low-elevation counterparts. Thus, climate shifts increasing freezing spells may affect the structure of communities in this zone.

Recently, human activities have impacted biodiversity-rich forest in western Rwanda, creating a need to enhance restoration activities of degraded lands in the region. To evaluate the effects of reforestation activities on the community composition of soil-litter ants, research was conducted in Gishwati tropical montane forest, located in northern-western part of Rwanda. The ant fauna was studied in reforested lands dominated by regenerated native species and exotic tree species. Further, a primary forest made of native trees served as a reference. In each forest type, nine sampling points were used to sample ants. Ant specimens were collected using pitfalls, hand sampling and Winkler extractor. They were identified to subfamilies, genus and species levels using dichotomous keys, and also statistically analysed for species richness, diversity, evenness and community composition. We collected a total of 2,481 individuals from 5 subfamilies, 18 genera and 35 species. Higher abundance, diversity and species richness were found in soil-litter under natural primary and secondary forests dominated by regenerated native plant species compared to exotic tree forest. The ant community composition analysis indicated higher similarities in ant species sampled under primary native forest and secondary forest dominated by regenerated native species. Reforestation by regenerating native species may be given priority in restoration of degraded lands due to their importance in species richness and species diversity.

Cerberiopsis candelabra Vieill. is a long-lived, monocarpic (= semelparous) and mass-flowering rain-forest tree, endemic to New Caledonia. Population size structures suggest establishment has been episodic, followed by a recruitment gap that might signal population decline. Here, we use age structures based on tree rings to better assess population dynamics and persistence, and investigate influences of tree size, age and growth rate on flowering. Age structures of populations surveyed in 2007–2008 were unimodal, with establishment over c. 15–81 y, followed by a recruitment gap of c. 23–79 y. Seedling mortality was generally high. High densities of flowering trees or large-scale exogenous disturbances may be necessary for in-situ regeneration. There was no evidence of a simple flowering threshold: flowering in 2017 occurred across a wide range of tree size, age and growth rate. Instead, evidence suggested that size and age at flowering may vary among plants depending on their growth trajectory. Environmental triggers of flowering were not identified by dating tree establishment, but the last three mass-flowering events occurred in years of tropical cyclones. Regeneration and persistence might be facilitated if large-scale disturbances trigger flowering, improving reproductive efficiency by synchronising flowering and linking reproduction with environmental conditions that enhance seedling recruitment.

In seasonal wet Neotropical forests, many studies have suggested that species-rich terrestrial frog assemblages are regulated bottom-up by the abundance of leaf litter. However, terrestrial frogs are prey to a diverse community of predators, and no studies have tested for top-down effects of predators on this or other anuran assemblages. Here, we used an extensive field dataset to model the relative contribution of food resources, microhabitat resources and predators towards the occupancy and detection of two frog species (Craugastor bransfordii and Oophaga pumilio) at La Selva, Costa Rica. Frog occupancy was most strongly influenced by predatory spiders and secondarily influenced by the abundance of leaf litter. Predators exerted stronger effects on frogs than food resources, and frogs avoided predators more as leaf litter decreased. Detection probability was elevated when predators were present. We found support for bottom-up effects of leaf litter on the terrestrial frog assemblage, but top-down effects by predators exerted stronger effects on frog occupancy and detection. Because predator avoidance varied along a resource gradient, predator and resource effects appear to be dependent, supporting interactions between top-down and bottom-up mechanisms. Climate-driven decreases in leaf litter may drive decreased availability of frog refugia and increased interactions between frogs and predators.

The temporal pattern of flower opening and closure is a feature of the biology of many plant species, particularly those inhabiting oceanic islands where flowering generally lasts for only a few hours per day. Additionally, flower visitors often seek different floral sources on a timely basis, thus the relative timing of interactions is central to their status in pollination competition, or in the facilitation of pollination among co-flowering plants sharing pollinators. However, few studies have examined the impacts of daily temporal variation in flowering patterns on the pollinator network and competition on a community scale. In order to examine whether the daily pattern of flower opening and closure can impose temporal dynamics on interspecific interactions within a single day, plant–pollinator interaction networks (AM subweb and PM subweb) were quantified, and the relevant interactions between the two subwebs were compared using the Bray–Curtis dissimilarity of visitation frequencies in an oceanic island community (Paracel Islands, South China Sea). The role of species within networks and its variation between two subwebs were assessed by calculating the species-level specialization and species strength of each plant and pollinator species. The quantitative plant–pollinator interaction dissimilarity between morning and afternoon subsets was 0.69, and this value dropped to 0.58 when considering plant species flowering throughout the day. In our study, this dissimilarity between the two subwebs might be explained by the morning peak activity rather than a preference for morning flowers. No significant differences were detected in the species-level specialization and species strength of plants flowering all day from morning to afternoon at the community level. The flower visitation rates of native honeybee Apis cerana were not significantly different between morning and afternoon for most of the whole-day flowering plants. However, plant species only flowering either in the morning or the afternoon differed in the rate of visitation by A. cerana. The analyses of variation in the visitation rates of pollinators shared by plants within a single day in the studied community suggest that daily structuring at a community level and half-day staggered flowering during the morning or afternoon might reduce competitive interactions in oceanic insular habitats.

IPCC predictions for Honduras indicate that temperature will increase by up to 3–6°C and precipitation will decrease by up to 7–13% by the year 2050. To better understand how fern and lycophyte communities might be affected by climate change, we comprehensively surveyed the community compositions of ferns and lycophytes at Celaque National Park, the highest mountain in Honduras. We surveyed a total of 80 20 × 20 m2 plots along an altitudinal gradient of 1249–2844 m a.s.l., identifying all species and estimating their abundances. We recorded a total of 11,098 individuals from 160 species and 61 genera. Community composition was strongly influenced by changes in altitude, precipitation and the abundance of bryophytes (a proxy for air humidity). Of the 160 species, 63 are expected, under a RCP2.6 scenario for the year 2050, to shift their range fully or partially above the maximum altitude of the mountain. Of these, 65.1% are epiphytes. We found that species with narrow altitudinal ranges at high altitudes were more at risk. Our study indicated that conservation efforts should prioritise higher altitudinal sites, focusing particularly on preserving the vulnerable epiphytic fern species, which are likely to be at greater risk.

Major biogeographic and taxonomic biases are recurrent in biological surveys, including fragmentation studies. Detecting biases and subsequent gaps is crucial to steer future research and suitable conservation policies. We evaluated biogeographic and fragmentation-related biases on antbirds and non-flying small mammals in Brazil, two oversampled and vulnerable taxonomic groups, by surveying papers in the Scielo and the Web of Science. We found 566 articles published from 1945 to 2018, including 55 and 43 fragmentation studies for antbirds and small mammals, respectively. Considering the species richness for each group across the Brazilian biomes, the number of publications for small mammals tended to disproportionately increase while increasing richness. The Atlantic Forest, the most degraded and densely populated biome, contained the highest number of publications. However, the Amazon included a disproportionately high number of papers considering its low population density. Conversely, non-forest biomes such as the Caatinga, Pampa and Pantanal were mostly overlooked. Our results show that research effort for small mammals and antbirds in Brazil is biogeographically biased. We call future research to consider more studies across non-forest biomes and vast unexplored areas within forest biomes to overcome major knowledge gaps on diversity, distribution and ecology of antbirds and small mammals in Brazil.

Mosses are one of the most important and dominant plant communities, especially in the temperate biome, and play a significant role in ecosystem function and dynamics. They influence the water, energy and element cycle due to their unique ecology and physiology. The present study was undertaken in three different temperate forest sites in the Garhwal Himalayas, viz., Triyuginarayan (Kedarnath Wildlife Sanctuary (KWLS)), Chakrata, and Kanasar forest range. The study was focused on understanding the influence of mosses on soil physical properties and nutrient availability. Different physico-chemical properties were analysed under two different substrata, that is, with and without moss cover in two different seasons, viz., monsoon and winter. We observed mosses to influence and alter the physical properties and nutrient status of soil in both seasons. All soil physical and chemical properties, except magnesium, showed significant difference within the substrates, among all the sites and across the two seasons. Besides the soil characteristics underneath the moss vegetation, the study also highlights the diversity of mosses found in the area. Mosses appear to create high nutrient microsites via a high rate of organic matter accumulation and retain nutrients for longer periods thus, maintaining ecosystem stability.

Knowledge on cheetah population densities across their current range is limited. Therefore, new and efficient assessment tools are needed to gain more knowledge on species distribution, ecology and behaviour. Scat detection dogs have emerged as an efficient and non-invasive method to monitor elusive and vulnerable animal species, like cheetahs, due to the dog’s superior olfactory system. However, the success of locating scat using detection dogs can be significantly improved under suitable weather conditions. We examined the impact of temperature, humidity and wind speed on detection rates of scat from cheetahs during a scat detection dog survey in Northern Kenya. We found that average wind speed positively influences the scat detection rate of detection dogs working on leash. Humidity showed no significant influence. Temperature showed a strong negative correlation with humidity and thus was excluded from our model analyses. While it is likely that wind speed is especially invalid for dogs working off leash, this study did not demonstrate this. Wind speed could thus influence the success of monitoring cheetahs or other target species. Our findings help to improve the survey and thus maximise the coverage of study area and the collection of target samples of elusive and rare species.

The unavoidable impact of roads on arboreal fauna in protected areas has received little attention. We investigated this impact on two gibbon species in Khao Yai National Park, Thailand: two groups had home ranges traversed by roads (roadside groups) and another two lived nearby roads (interior groups). Roads partially delineated the edges of home ranges of roadside groups, and gibbons crossed them only at a few locations. Gibbons’ space use decreased near roads for roadside groups and showed road reluctance as their crossing rates were smaller than those produced by a null movement model. Generalised linear models (GLMs) indicated that a long canopy gap reduced gibbons’ crossing probability, whereas forest cover had a positive effect. A large part of the road network had a low probability of being crossed by gibbons according to GLMs, especially at areas around park headquarters. Roads were still relatively permeable to gibbon movement with a mean 35% crossing probability. The relatively short and narrow road network in the park constitutes a positive assessment of the standards of how roads should be built in protected areas. Nonetheless, this assessment might be the consequence of the park being set in a mountainous region with difficulties of road development.

The importance of wild insects as pollinators of tropical tree crops has rarely been tested. Across 18 small-scale lychee orchards in northern Thailand, we evaluated the roles of different wild insects as pollinators and predators of pests in fruit production. Quantitative assessments showed that bees (Family Apidae) were strongly dominant (83%) among insect flower visitors, comprising four species in tribes Apini and four in Meliponini. Experimental manipulations of inflorescences showed that fruit production in these orchards was: (1) dependent on flower visits by wild insects because enclosure of inflorescences in mesh bags decreased fruit set (to one-fifth) and (2) not greatly limited by pollinator deficiencies, because hand pollination of unbagged flowers did not enhance fruit set. Pollination success, as indicated by the proportion of unmanipulated flowers setting fruit, correlated positively across orchards with the abundance of large-bodied Apidae (>7 mm; most were Apis species) and of Apini, and negatively with abundance of small-bodied Apidae and of all Meliponini, despite the latter being the commonest flower visitors. We conclude that larger-bodied bees are most likely to travel sufficiently far to import genetically diverse pollen, in this landscape-scale mosaic where non-orchard habitats (both agriculture and treed patches) were sufficient to sustain wild pollinators.

The spatial distribution of Neotropical birds at local scales is often associated with plant successional stages and unique microhabitats such as bamboo stands and treefall gaps. Only 15% of the Atlantic Forest remains, yet this habitat is home to more than 200 endemic birds. Understanding the distribution of bird species within the Atlantic Forest can improve the test of ecological hypotheses related to habitat use and habitat selection. This study analyses the distribution of birds among different types of relief (lowlands, ravines, hillsides, and hilltops) in 41 forest fragments of different sizes (1 to 384.5 ha) within the Atlantic Forest, south east Brazil. The presence and absence data were analysed with dynamic occupancy models to determine the influence of area and topography in the distribution of bird species. Of the 169 species detected, we were able to estimate an occupancy probability of 67. Among them, relief type was an important variable for 26 species (39%). Most species showed a preference for lowlands and ravines, and some showed an association of combinations of these two reliefs with hillsides and hilltops. These preferences might be related to distribution of preferred food, nesting microhabitats, and thermoregulation. For the endemic and threatened Atlantic Forest mountain birds, the ecological association with areas of relief is a strong, and previously unsuspected, spatial distribution pattern. Our results suggest that the ecology and distribution of birds in tropical forests across hilly regions could be better understood if topography is considered. This pattern should be better investigated among other taxa in tropical forests.