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Analysis of wild Lactuca accessions: conservation and identification of redundancy

Published online by Cambridge University Press:  25 June 2008

Tatjana Sretenović Rajičić ,
Theo van Hintum ,
Aleš Lebeda  and
Klaus J. Dehmer
Tatjana Sretenović Rajičić*
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben, Germany
Theo van Hintum
Affiliation:
Centre for Genetic Resources, The Netherlands (CGN), Wageningen University and Research Centre, PO Box 16, 6700 AA Wageningen, The Netherlands
Aleš Lebeda
Affiliation:
Department of Botany, Faculty of Science, Palacký University, Slechtitelu 11, 783 71 Olomouc-Holice, Czech Republic
Klaus J. Dehmer
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstr. 3, 06466 Gatersleben, Germany
*
*Corresponding author. Email t.rajicic@googlemail.com; rajicic@ipk-gatersleben.de
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Abstract

Germplasm accessions of wild Lactuca species are maintained worldwide in ex situ collections as gene reservoirs for quality and disease resistance traits for cultivated lettuce. Accessions of 12 Lactuca species from 6 genebanks were compared via morphological characterization and AFLP (Amplified Fragment Length Polymorphism)-based profiling to estimate the extent of duplication. A method of assessing redundancy within very similar, but not identical accessions, is proposed, based on 352 polymorphic AFLP products. Seven duplication groups showed a high level of AFLP similarity, and one pair of Lactuca saligna accessions displayed identical AFLP profiles. In several cases, the morphological assessment indicated that a taxonomic reclassification of accessions was necessary. Candidate duplicates were identified using population parameters and inter- and intra-accession variability. The implications of these findings on the conservation of wild species are discussed.

Keywords

AFLPduplicationplant genetic resources
Type
Research Article
Information
Plant Genetic Resources , Volume 6 , Issue 2 , August 2008 , pp. 153 - 163
Copyright
Copyright © NIAB 2008

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