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Prevalence of puroindoline alleles in wheat varieties from eastern Asia including the discovery of a new SNP in puroindoline b

Published online by Cambridge University Press:  16 May 2008

Hiroyuki Tanaka ,
Craig F. Morris ,
Mika Haruna  and
Hisashi Tsujimoto
Hiroyuki Tanaka*
Affiliation:
Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
Craig F. Morris
Affiliation:
USDA-ARS Western Wheat Quality Laboratory, Pullman, WA 99164-6394, USA
Mika Haruna
Affiliation:
Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
Hisashi Tsujimoto
Affiliation:
Laboratory of Plant Genetics and Breeding Science, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
*
*Corresponding author. E-mail: htanaka@muses.tottori-u.ac.jp
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Abstract

Kernel texture (grain hardness) in common wheat (Triticum aestivum L.) is of primary technological importance and is largely determined by puroindoline gene sequence and expression. We investigated the puroindoline haplotypes of 246 Asian common wheat varieties. All but three were conclusively characterized for puroindoline a and b haplotypes. Of the total, 174 possessed the soft Pina-D1a/Pinb-D1a ‘wild-type’ gene sequences with SKCS hardness indexes (HI) ranging from 13.5 to 61.8. Among the remaining 72 varieties with HIs of 56.1–97.8, nearly half (30) were Pina-D1a/Pinb-D1b, 4 were Pina-D1a/Pinb-D1c, 19 were Pina-D1a/Pinb-D1p, 10 were Pina-D1b/Pinb-D1a (‘a-null’), 3 were Pina-D1l/Pinb-D1a, 2 possessed a new C-to-T SNP mutation at position 382, which is tentatively designated Pinb-D1ab, 1 was a ‘double null’ with neither puroindoline a nor b expressed and no PCR-detectable gene sequence, and 3 had undetermined/ambiguous puroindoline a sequence but possessed Pinb-D1a. The double null was the hardest of all varieties tested with an HI of 97.8. The frequency of soft and hard varieties and puroindoline hardness haplotype varied depending on the origin of the varieties. The lowest frequency of hard varieties occurred in Korea and south-western Japan. Tibet and Pakistan also had low frequencies of hard varieties. The highest frequency of hard varieties appeared in north-east China followed by north-west China and Nepal. Within Asia, the Pinb-D1p allele appears in a region extending from north-eastern China through Inner Mongolia, north-western China, Xinjiang and Tibet, with the greatest frequency in north-western China. This allele was also present in Pakistan and Afghanistan, but not found in Japan, and may have been dispersed along the ‘Silk Road’. All three Pina-D1l varieties came from China. The newly discovered SNP originated in Afghanistan and the ‘double null’ in Xinjiang.

Keywords

grain hardnessmutationpuroindolineTriticum aestivum Lwheat
Type
Research Article
Information
Plant Genetic Resources , Volume 6 , Issue 2 , August 2008 , pp. 142 - 152
Copyright
Copyright © NIAB 2008

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