Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-13T07:27:56.591Z Has data issue: false hasContentIssue false
  • English
  • Français

Regulation of fatty acid transport by fatty acid translocase/CD36

Published online by Cambridge University Press:  05 March 2007

Arend Bonen ,
Shannon E. Campbell ,
Carley R. Benton ,
Adrian Chabowski ,
Susan L. M. Coort ,
Xiao-Xia Han ,
Debby P. Y. Koonen ,
Jan F. C. Glatz  and
Joost J. F. P. Luiken
Arend Bonen*
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
Shannon E. Campbell
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada Department of Kinesiology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada
Carley R. Benton
Affiliation:
Department of Kinesiology, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada
Adrian Chabowski
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
Susan L. M. Coort
Affiliation:
Department of Molecular Genetics, CARIM, Maastricht University, 6200 MD, Maastricht, The Netherlands
Xiao-Xia Han
Affiliation:
Department of Human Biology and Nutritional Sciences, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
Debby P. Y. Koonen
Affiliation:
Department of Molecular Genetics, CARIM, Maastricht University, 6200 MD, Maastricht, The Netherlands
Jan F. C. Glatz
Affiliation:
Department of Molecular Genetics, CARIM, Maastricht University, 6200 MD, Maastricht, The Netherlands
Joost J. F. P. Luiken
Affiliation:
Department of Molecular Genetics, CARIM, Maastricht University, 6200 MD, Maastricht, The Netherlands
*
*Corresponding author: Dr Arend Bonen Fax: +1 519 763 59002, Email: abonen@uoguelph.ca
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Fatty acid (FA) translocase (FAT)/CD36 is a key protein involved in regulating the uptake of FA across the plasma membrane in heart and skeletal muscle. A null mutation of FAT/CD36 reduces FA uptake rates and metabolism, while its overexpression increases FA uptake rates and metabolism. FA uptake into the myocyte may be regulated (a) by altering the expression of FAT/CD36, thereby increasing the plasmalemmal content of this protein (i.e. streptozotocin-induced diabetes, chronic muscle stimulation), or (b) by relocating this protein to the plasma membrane, without altering its expression (i.e. obese Zucker rats). By repressing FAT/CD36 expression, and thereby lowering the plasmalemmal FAT/CD36 (i.e. leptin-treated animals), the rate of FA transport is reduced. Within minutes of beginning muscle contraction or being exposed to insulin FA transport is increased. This increase is a result of the contraction- and insulin-induced translocation of FAT/CD36 from an intracellular depot to the cell surface. Neither PPARα nor PPARγ activation alter FAT/CD36 expression in muscle, despite the fact that PPARα activation increases FAT/CD36 by 80% in liver. A novel observation is that FAT/CD36 also appears to be involved in mitochondrial FA oxidation, as this protein is located on the mitochondrial membrane and seems to be required to participate in moving FA across the mitochondrial membrane. Clearly, FAT/CD36 has an important role in FA homeostasis in skeletal muscle and the heart.

Keywords

CardiacMyocytesSkeletal muscleGiant vesicles
Type
Symposium 2: The fatty acid transporters of skeletal muscle
Information
Proceedings of the Nutrition Society , Volume 63 , Issue 2 , May 2004 , pp. 245 - 249
Copyright
Copyright © The Nutrition Society 2004

References

Bonen, A, Dyck, DJ, Ibrahimi, A & Abumrad, NA (1999) Muscle contractile activity increases fatty acid metabolism and transport and FAT/CD36. American Journal of Physiology 276, E642–E649.Google ScholarPubMed
Bonen, A, Glatz, JFC & Luiken, JJFP (2002) Regulation of fatty acid transport and membrane transporters in health and disease. Molecular and Cellular Biochemistry 239, 181–192.CrossRefGoogle ScholarPubMed
Bonen, A, Luiken, JJFP, Arumugam, Y, Glatz, JFC & Tandon, NN (2000) Acute regulation of fatty acid uptake involves the cellular redistribution of fatty acid translocase. Journal of Biological Chemistry 275, 14501–14508.CrossRefGoogle ScholarPubMed
Bonen, A, Luiken, JJFP, Lui, S, Dyck, DJ, Kiens, B, Kristiansen, S, Turcotte, LP van der Vusse, GJ & Glatz, JFC (1998) Palmitate transport and fatty acid transporters in red and white muscles. American Journal of Physiology 275, E471–E478.Google ScholarPubMed
Brinkman, J (2003) Functional significance of fatty acid translocase (FAT/CD36) in rodent cardiac muscle. PhD Thesis, Maastricht University, The Netherlands.Google Scholar
Febbraio, M, Abumrad, NA, Hajjat, DP, Sharma, K, Cheng, W, Frieda, S, Pearce, A & Silverstein, RL (1999) A null mutation in murine CD36 reveals an important role in fatty acid and lipoprotein metabolism. Journal of Biological Chemistry 274, 19055–19062.CrossRefGoogle Scholar
Hamilton, JA & Kamp, F (1999) How are free fatty acids transported in membranes? Is it by proteins or by free diffusion through the lipids. Diabetes 48, 2255–2269.CrossRefGoogle ScholarPubMed
Ibrahimi, A, Bonen, A, Blinn, WD, Hajri, T, Li, X, Zhong, K, Cameron, R & Abumrad, NA (1999) Muscle-specific overexpression of FAT/CD36 enhances fatty acid oxidation by contracting muscles, reduces plasma triglycerides and fatty acids, and increases plasma glucose and insulin. Journal of Biological Chemistry 274, 26761–26766.CrossRefGoogle ScholarPubMed
Lee, C-H, Olson, P & Evans, RM (2003) Lipid metabolism, metabolic diseases, and peroxisome proliferators-activated receptors. Endocrinology 144, 2201–2207.CrossRefGoogle Scholar
Luiken, JJFP, Arumugam, Y, Bell, RC, Calles-Escandon, J, Tandon, NN, Glatz, JFC & Bonen, A (2002a) Changes in fatty acid transport and transporters are related to the severity of insulin deficiency. American Journal of Physiology 282, 612–621.Google Scholar
Luiken, JJFP, Arumugam, Y, Dyck, DJ, Bell, RC, Pelsers, ML, Turcotte, LP, Tandon, NN, Glatz, JFC & Bonen, A (2001) Increased rates of fatty acid uptake and plasmalemmal fatty acid transporters in obese Zucker rats. Journal of Biological Chemistry 276, 40567–40573.CrossRefGoogle ScholarPubMed
Luiken, JJFP, Dyck, DJ, Han, X-X, Tandon, NN, Arumugam, Y, Glatz, JFC & Bonen, A (2002b) Insulin induces the translocation of the fatty acid transporter FAT/CD36 to the plasma membrane. American Journal of Physiology 282, E491–E495.Google ScholarPubMed
Luiken, JJFP, Koonen, DPY, Willems, J, Zorzano, A, Fischer, Y van der Vusse, GJ, Bonen, A & Glatz, JFC (2002c) Insulin stimulates long-chain fatty acid utilization by rat cardiac myocytes through cellular redistribution of FAT/CD36. Diabetes 51, 3113–3119.CrossRefGoogle ScholarPubMed
Luiken, JJFP, Turcotte, LP & Bonen, A (1999) Protein-mediated palmitate uptake and expression of fatty acid transport proteins in heart giant vesicles. Journal of Lipid Research 40, 1007–1016.CrossRefGoogle ScholarPubMed
Schaffer, JE (2002) Fatty acid transport: the roads taken. American Journal of Physiology 282, E239–E246.Google ScholarPubMed
Steinberg, GR, Dyck, DJ, Calles-Escandon, J, Tandon, NN, Luiken, JJFP, Glatz, JFC & Bonen, A (2002) Chronic leptin administration decreases fatty acid uptake and fatty acid transporters in rat skeletal muscle. Journal of Biological Chemistry 277, 8854–8860.CrossRefGoogle ScholarPubMed
Teboul, L, Febbraio, M, Gaillard, D, Amri, Z, Silverstein, RL & Grimaldi, PA (2001) Structural and functional characterization of the mouse fatty acid translocase promoter: activation during adipose differentiation. Biochemical Journal 360, 305–312.CrossRefGoogle ScholarPubMed