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Identification of quantitative trait loci controlling seed physical and nutrient traits in cotton

Published online by Cambridge University Press:  01 December 2007

Xian-Liang Song  and
Tian-Zhen Zhang
Xian-Liang Song
Affiliation:
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China College of Agronomy, State Key Laboratory of Crop Biology, Shandong Agricultural University, Taian 271018, China
Tian-Zhen Zhang*
Affiliation:
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China
*
*Correspondence Fax: 0086 25 84395307 E-mail: cotton@njau.edu.cn
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Abstract

Cotton (Gossypium spp.) is an important source of edible oil and protein meals. Complex genetics and strong environmental effects hinder progress in seed quality trait breeding in this species. The use of molecular markers can improve an understanding of the genetic factors conditioning seed quality traits, and is expected to assist in selection of superior genotypes. This study was conducted to identify quantitative trail loci (QTL) associated with seed physical and nutrient traits in cotton. To achieve this objective, a population of 140 BC1S1 lines developed from a cross between ‘TM-1’ and ‘Hai7124’ was evaluated in 2003 and 2004. A linkage map consisting of 918 markers from this population was used to identify QTL using QTLNetwork-2.0 software. Eleven single QTL were identified for kernel percentage, kernel oil percentage, kernel protein percentage and seven amino acids (Asp, Ser, Gly, Ile, Leu, Phe and Arg). Phenotypic variation explained by each individual QTL ranged from 10.89 to 46.28%. Two epistatic QTL for Cys and Leu were detected, explaining 9.55 and 4.43% of the phenotypic variation. These QTL detected for seed quality traits in cotton are expected to be useful for further breeding programmes targeting development of cotton with improved nutrient quality.

Keywords

amino acidscottonGossypiummarker-assisted selectionquantitative trait loci mappingseed oilseed protein
Type
Research Article
Information
Seed Science Research , Volume 17 , Issue 4 , December 2007 , pp. 243 - 251
Copyright
Copyright © Cambridge University Press 2007

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References

Alrefai, R., Berke, T.G. and Rocheford, T.R. (1995) Quantitative trait locus analysis of fatty acid concentrations in maize. Genome 38, 894–901.CrossRefGoogle ScholarPubMed
Bert, P.-F., Jouan, I., Tourvieille de Labrouhe, D., Serre, F., Philippon, J., Nicolas, P. and Vear, F. (2003) Comparative genetic analysis of quantitative traits in sunflower (Helianthus annuus L.). 2. Characterisation of QTL involved in developmental and agronomic traits. Theoretical and Applied Genetics 107, 181–189.CrossRefGoogle ScholarPubMed
Brummer, E.C., Graef, G.L., Orf, J., Wilcox, J.R. and Shoemaker, R.C. (1997) Mapping QTL for seed protein and oil content in eight soybean populations. Crop Science 37, 370–378.Google Scholar
Chen, Z.F., Zhang, Z.W. and Cheng, H.L. (1986) The analysis of Upland cotton quality. Acta Agronomica Sinica 12, 195–200.Google Scholar
Chung, J., Babka, H.L., Graef, G.L., Staswick, P.E., Lee, D.J., Cregan, P.B., Shoemaker, R.C. and Specht, J.E. (2003) The seed protein, oil, and yield QTL on soybean linkage group I. Crop Science 43, 1053–1067.Google Scholar
Dani, R.G. and Kohel, R.J. (1989) Maternal effects and generation mean analysis of seed-oil content in cotton (Gossypium hirsutum L.). Theoretical and Applied Genetics 77, 569–575.CrossRefGoogle ScholarPubMed
Diers, B.W., Keim, P., Fehr, W.R. and Shoemaker, R.C. (1992) RFLP analysis of soybean seed protein and oil content. Theoretical and Applied Genetics 83, 608–612.Google Scholar
D'Mello, J.P.F. (2003) Amino acids as multifunctional molecules. pp. 1–4in D'Mello, J.P.F. (Ed.) Amino acids in animal nutrition (2nd edition). Wallingford, UK, CABI Publishing.CrossRefGoogle Scholar
Falconer, D.S. and Mackay, T.F.C. (1996) Introduction to quantitative genetics (4th edition). Harlow, UK, Longmans Green.Google Scholar
Gao, G.Q., Lu, T.X., Su, X.H., Liu, Y.X., Wu, D.F. and Zhu, B.D. (2003) Seed protein electrophoresis for identification of fine fiber cotton line. Acta Agriculturae Nucleatae Sinica 5, 340–342.Google Scholar
Goldman, I.L., Rocheford, T.R. and Dudley, J.W. (1994) Molecular markers associated with maize kernel oil concentration in an Illinois high protein ×  Illinois low protein cross. Crop Science 34, 908–915.CrossRefGoogle Scholar
Gupta, V., Mukhopadhyay, A., Arumugam, N., Sodhi, Y.S., Pental, D. and Pradhan, A.K. (2004) Molecular tagging of erucic acid trait in oilseed mustard (Brassica juncea) by QTL mapping and single nucleotide polymorphisms in FAE1 gene. Theoretical and Applied Genetics 108, 743–749.Google Scholar
Han, Z.G., Guo, W.Z., Song, X.L. and Zhang, T.Z. (2004) Genetic mapping of EST-derived microsatellites from the diploid Gossypium arboreum in allotetraploid cotton. Molecular Genetics and Genomics 272, 308–327.Google Scholar
Han, Z.G., Wang, C.B., Song, X.L., Guo, W.Z., Gou, J.Y., Li, C.H., Chen, X.Y. and Zhang, T.Z. (2006) Characteristics, development and mapping of Gossypium hirsutum derived EST-SSRs in allotetraploid cotton. Theoretical and Applied Genetics 112, 430–439.Google Scholar
Hu, X., Sullivan-Gilbert, M., Gupta, M. and Thompson, S.A. (2006) Mapping of the loci controlling oleic and linolenic acid contents and development of fad2 and fad3 allele-specific markers in canola (Brassica napus L.). Theoretical and Applied Genetics 113, 497–507.Google Scholar
Jiang, C., Wright, R.J., Woo, S.S., Delmonte, T.A. and Paterson, A.H. (2000) QTL analysis of leaf morphology in tetraploid Gossypium (cotton). Theoretical and Applied Genetics 100, 409–418.Google Scholar
Kianian, S.F., Egli, M.A., Phillips, R.L., Rines, H.W., Somers, D.A., Gengenbach, B.G., Webster, F.H., Livingston, S.M., Groh, S., O'Donoughue, L.S., Sorrells, M.E., Wesenberg, D.M., Stuthman, D.D. and Fulcher, R.G. (1999) Association of a major groat oil content QTL and an acetyl-CoA carboxylase gene in oat. Theoretical and Applied Genetics 98, 884–894.CrossRefGoogle Scholar
Kohel, R.J. and Cherry, J.P. (1983) Variation of cottonseed quality with stratified harvests. Crop Science 23, 1119–1124.CrossRefGoogle Scholar
Mansur, L.M., Lark, K.G., Kross, H. and Oliveira, A. (1993) Interval mapping of quantitative trait loci for reproductive, morphological, and seed traits of soybean (Glycine max L.). Theoretical and Applied Genetics 86, 907–913.CrossRefGoogle ScholarPubMed
Panthee, D.R., Pantalone, V.R., West, D.R., Saxton, A.M. and Sams, C.E. (2005) Quantitative trait loci for seed protein and oil concentration, and seed size in soybean. Crop Science 45, 2015–2022.Google Scholar
Panthee, D.R., Pantalone, V.R., Saxton, A.M., West, D.R. and Sams, C.E. (2006) Genomic regions associated with amino acid composition in soybean. Molecular Breeding 17, 79–89.CrossRefGoogle Scholar
Pérez-Vich, B., Knapp, S.J., Leon, A.J., Fernández-Martínez, J.M. and Berry, S.T. (2004) Mapping minor QTL for increased stearic acid content in sunflower seed oil. Molecular Breeding 13, 313–322.CrossRefGoogle Scholar
See, D., Kanazin, V., Kephart, K. and Blake, T. (2002) Mapping genes controlling variation in barley grain protein concentration. Crop Science 42, 680–685.Google Scholar
Singh, M., Singh, T.H. and Chahal, G.S. (1985) Genetic analysis of some seed quality characters in upland cotton (Gossypium hirsutum L.). Theoretical and Applied Genetics 71, 126–128.CrossRefGoogle ScholarPubMed
Song, X.L., Wang, K., Guo, W.Z., Zhang, J. and Zhang, T.Z. (2005) A comparison of genetic maps constructed from haploid and BC1 mapping populations from the same crossing between Gossypium hirsutum L. and Gossypium barbadense L. Genome 48, 378–390.Google Scholar
Sun, S.K., Chen, J.H., Xian, S.K. and Wei, S.J. (1987) Study on the nutritional quality of cotton seeds. Scientia Agricultura Sinica 5, 12–16.Google Scholar
Tan, Y.F., Sun, M., Xing, Y.Z., Hua, J.P., Sun, X.L., Zhang, Q.F. and Corke, H. (2001) Mapping quantitative trait loci for milling quality, protein content and color characteristics of rice using a recombinant inbred line population derived from an elite rice hybrid. Theoretical and Applied Genetics 103, 1037–1045.CrossRefGoogle Scholar
Tanhuanpaa, P.K., Vilkki, J.P. and Vilkki, H.J. (1995a) Identification of a RAPD marker for palmitic acid concentration in the seed of spring turnip rape (Brassica rapa ssp oleifera). Theoretical and Applied Genetics 91, 477–480.Google Scholar
Tanhuanpaa, P.K., Vilkki, J.P. and Vilkki, H.J. (1995b) Association of a RAPD marker with linolenic acid concentration in the seed oil of rapeseed (Brassica napus L.). Genome 38, 414–416.CrossRefGoogle ScholarPubMed
Tar'an, B., Warkentin, T., Somers, D.J., Miranda, D., Vandenberg, A., Blade, S. and Bing, D. (2004) Identification of quantitative trait loci for grain yield, seed protein concentration and maturity in field pea (Pisum sativum L.). Euphytica 136, 297–306.Google Scholar
Wang, K., Song, X.L., Han, Z.G., Guo, W.Z., Yu, J.Z., Sun, J., Pan, J.J., Kohel, R.J. and Zhang, T.Z. (2006) Complete assignment of the chromosomes of Gossypium hirsutum L. by translocation and fluorescence in situ hybridization mapping. Theoretical and Applied Genetics 113, 73–80.Google Scholar
Wang, X.L. and Larkins, B.A. (2001) Genetic analysis of amino acid accumulation in opaque-2 maize endosperm. Plant Physiology 125, 1766–1777.CrossRefGoogle ScholarPubMed
Ye, Z.H., Lu, Z.Z. and Zhu, J. (2003) Genetic analysis for developmental behavior of some seed quality traits in Upland cotton (Gossypum hirsutum L.). Euphytica 129, 183–191.CrossRefGoogle Scholar
Zhang, C.Q., Yin, Y.P., Gao, R.Q. and Jia, J.Z. (1998) Polymorphism of seed protein and identification of cotton cultivar. Scientia Agricultura Sinica 31, 16–19.Google Scholar
Zhao, J.Y., Becker, H.C., Zhang, D.Q., Zhang, Y.F. and Ecke, W. (2006) Conditional QTL mapping of oil content in rapeseed with respect to protein content and traits related to plant development and grain yield. Theoretical and Applied Genetics 113, 33–38.Google Scholar
Zhu, X.C., Zhao, W.M. and Liao, X.R. (1995) Varieties of Gossypium seed protein components. Acta Gossypium Sinica 4, 223–225.Google Scholar