The population declines affecting Asian Gyps vultures are among the most rapid and geographically widespread recorded for any species. This paper describes the rates and patterns of mortality and population change over 4 years at three Oriental white-backed vulture Gyps bengalensis colonies in Pakistan: Dholewala (initially 421 pairs), Toawala (initially 445 pairs) and Changa Manga (initially 758 pairs). Vulture mortality led to the extirpation of two of these colonies (Changa Manga and Dholewala) in 3 years, and a decline of 54.3% in the third. Visceral gout, indicative of diclofenac poisoning, was the largest single cause of death in vultures examined. Annual adult mortality from diclofenac poisoning was significantly positively correlated with annual population declines at each colony indicating a direct causal relationship. Visceral gout occurred in temporal and spatial clusters suggesting multiple point sources of diclofenac exposure. The spatial and temporal distribution of dead vultures and approximate time since death were used to estimate minimum rates at which colonies encountered carcasses with sufficient diclofenac to cause mortality of 1.26–1.88 carcasses per colony per month. By estimating total carcass consumption at each colony, the percentage of carcasses contaminated with diclofenac was calculated as 1.41–3.02%, exceeding the minimum required to have caused the observed population decline. With populations declining by approximately 50% annually, the long term survival of Gyps vultures in South Asia will require the removal of diclofenac from vulture food and establishment of captive populations for future restoration once the environment is free from contamination.

Systematic conservation planning is widely considered the most effective approach for designing protected area and other ecological networks. However, many conservation practitioners still ignore these methods and we suggest that five perceived limitations of this process are affecting its uptake. These perceptions are that (1) systematic conservation planning software is difficult to use, (2) the process requires extensive biodiversity distribution data, (3) setting targets for representing conservation features is not possible, (4) the advantages of systematic conservation planning do not outweigh the costs, and (5) the resulting plans often identify unsuitable areas. Here we review these perceived limitations and argue they are all misplaced, although we recognize difficulties in the target setting process. We then illustrate the value of systematic conservation planning to practitioners using a case study that describes a low-cost exercise from Maputaland, South Africa. This preliminary conservation assessment measured the effectiveness of the existing reserve system and identified a number of candidate areas that could be the focus of community- or privately-run ecotourism or game ranching ventures. Our results also emphasize both the importance of producing planning outputs that are specifically targeted for stakeholders, and the role of systematic conservation planning in providing a framework for integrating different provincial, national and transnational conservation initiatives.

Species reintroduction programmes, in prioritizing areas for reintroductions, have traditionally used tools that include measures of habitat suitability and evaluations of area requirements for viable populations. Here we add two tools to this approach: evaluation of ecological requirements of species and evaluation of future suitability for species facing changing climates. We demonstrate this approach with two species for which reintroduction programmes are in the planning stages in Mexico: California condor Gymnogyps californianus and Mexican wolf Canis lupus baileyi. For the condor, we identify three areas clustered in the Sierra San Pedro Mártir, Baja California; for the wolf, we identify a string of suitable sites along the Sierra Madre Occidental of western Mexico. We discuss the limitations of this approach, identifying ways in which the models illustrated could be made more realistic and directly useful to reintroduction programmes.

Mountain gorillas Gorilla beringei beringei are Critically Endangered, with just two small populations: in Bwindi Impenetrable National Park in south-western Uganda and the nearby Virunga Volcanoes on the borders with Rwanda and Uganda. A survey of the Bwindi population was carried out in 2002 and results were compared with the previous census in 1997. Our estimate of total population size increased over that period by c. 7% to 320 individuals and the structure and distribution of the population were largely unchanged. Signs of human disturbance were more common in 2002 than 1997, and gorillas tended to be found in areas of relative low disturbance. This suggested that disturbance could be a constraint on population growth and distribution but demographic stochasticity may also be responsible for the observed level of population change over a short time period. Other potential limiting factors, including habitat availability and disease, are discussed. While conservation activities in Bwindi have probably contributed to the stability of the population, strengthening of law enforcement and continued vigilance are needed to ensure the population's long-term growth and survival.

Mountain gorilla Gorilla beringei beringei tracking tourism generates important revenue for conservation efforts but brings with it the threat of disease transmission into the gorilla population. This study quantifies for the first time aspects of encounters between gorillas and tourists at Bwindi Impenetrable National Park that are likely to contribute to the risk of disease transmission. These include how close tourists get to gorillas, how close encounters are initiated, how long they last, and the age class of gorillas involved. Tourists got significantly closer to gorillas than the park rules allow (a mean of 2.76 m, compared to the rule of 7 m), and remained close for long periods. Contacts with the gorillas most vulnerable to disease, the juveniles, were closer but of shorter duration than those with adults. Contacts initiated by gorillas were closer but shorter than those initiated by tourists. Taken together these results demonstrate that the present rules are failing, and that the risk of disease transmission may be greater than previously believed.

Density dependence of the Addo Elephant National Park (South Africa) elephants Loxodonta africana was assessed using a long-term data set. Estimated carrying capacity is 0.1–0.5 elephants km−2 but stocking rates have been up to 4 elephants km−2. Population growth rate was found to be positively correlated with increasing density. There was no relationship between birth rate, the age of first calving or calf sex ratio and elephant density but there was a positive relationship between birth rate and rainfall during conception year. Mortality rates, particularly for juveniles, were low, and mean inter-calf interval was 3.3 years. There is no evidence of density dependent regulation in this population, despite the population being consistently above the estimated sustainable carrying capacity and a loss of phytomass and biodiversity. This is interpreted in light of the characteristics of the aseasonal habitat, succulent thicket vegetation and the ability of elephants to utilize accumulated vegetation biomass. These findings indicate that density dependence should not be considered as an option in the control of elephant numbers in this Park, or where elephant resources are not seasonally limited.

We carried out a nationwide survey of elephant Loxodonta africana in Guinea Bissau, a small West African country for which records of elephant are limited. We also investigated parts of western Guinea along the border with Guinea Bissau likely to harbour a transboundary elephant population. Standardized interviews with hunters were held in 110 villages in Guinea Bissau and 60 villages in Guinea, and field surveys were carried out to validate interviewee responses. Results suggest that elephants are mainly restricted to an area between the Corubal River (Guinea Bissau) and the Kogon River (Guinea) and that elephants occur only seasonally in Guinea. Based on the number, geographical localization and interpretation of observed tracks, our estimate of the minimum number of elephants in Guinea Bissau is 4–10 animals. We did not observe any signs of young elephants. The most immediate threat to elephants is a road scheme between Guinea Bissau and Guinea that cuts through elephant range. The future of elephants in this region depends on the capacity of the two countries to manage their common elephant population jointly. In particular, the creation of a transboundary park is urgently needed.

Knowledge of the conservation status of Bornean bats is biased towards areas considered to support high diversity in the northern Malaysian states. Few surveys have been undertaken in Indonesian Borneo, despite it representing over two thirds of the island's land area. We present the first description of a bat assemblage in Borneo's nutrient poor, or oligotrophic, forests (heath and peat swamp), habitats that have been considered depauperate in wildlife. We surveyed two protected areas in Central Kalimantan using harp traps supplemented by mist nets. We recorded 27 species, 18 of which were captured exclusively by harp traps. The bat assemblage of both sites was dominated by vespertilionids of the subfamily Kerivoulinae. The most abundant species, Kerivoula intermedia, accounted for 45% of standardized captures in Tanjung Puting National Park. At this site 15 species were represented by <10 captures each. Hipposideros ridleyi, Kerivoula lenis, Murina aenea and Murina rozendaali, four rare and threatened species, were recorded for the first time in Indonesia, and Phoniscus atrox, Murina cyclotis and Hipposideros doriae were recorded for the first time in Kalimantan. Estimation of species richness indicated that this inventory was almost complete for understorey, narrow-space, insectivores susceptible to capture in harp traps. It is likely that further surveys targeting open spaces, rivers, forest edges and the canopy will record species of other foraging guilds. The inventory exceeds those from other sites in Kalimantan because previous surveys have not used harp traps. However, it is a depauperate subset of assemblages studied in north Borneo, most likely because these sites are better known and are nearer to caves. Despite this relatively low diversity, our study shows that protected areas in oligotrophic forests provide valuable habitat for some of this island's rarest bat species, and are likely to become more important for bat conservation in light of widespread forest disturbance in Borneo.

The bumble-bee bat Craseonycteris thonglongyai of the monospecific family Craseonycteridae is categorized as Endangered on the IUCN Red List. First discovered in 1973, it was until recently only known from a small population of approximately 2,300 individuals restricted to the catchment area of the River Kwai, Thailand. However, in 2001 a single craseonycterid was discovered in Mon State, Myanmar, extending its geographical range by approximately 250 km. In October and November 2002 a survey was undertaken to examine the status of C. thonglongyai in Myanmar and assess its geographical distribution and population size. C. thonglongyai calls were recorded from bats emerging from nine of 19 caves surveyed; the population size was estimated to be 1,500. The phylogenetic relationships between the Thai and Myanmar populations were investigated using molecular, morphological and acoustic data. Morphologically, the two populations are indistinguishable. However, there is an 8–10 kHz echolocation call divergence between the populations. Cytochrome b data suggest that the two populations are similar and that the Myanmar population may be monophyletic. Annual surveys of the known bat roosts and in situ education programmes for local people are recommended together with the establishment of an integrated, transboundary Myanmar/Thai conservation strategy.

Extant representatives of the ancient crustacean family Anaspididae are restricted to the island State of Tasmania, Australia. Allanaspides hickmani and Allanaspides helonomus were first described in the early 1970s from surface pools in buttongrass moorland in two adjacent watersheds in south-west Tasmania. Both of these catchments have since been inundated for hydroelectric power generation (Lake Gordon and Serpentine Impoundments). Surveys indicate that both species persist in a small number of highly fragmented populations on the margins of the two impoundments. A. hickmani and A. helonomus have extant Areas of Occupancy of c. 21 and 54 km2, respectively. We estimate that inundation resulted in the loss of 85–94% of the original range of A. hickmani and c. 78% of the original range of A. helonomus. Under IUCN Red List guidelines and National threatened species legislation A. hickmani but not A. helonomus may qualify for listing as Vulnerable (Area of Occupancy <20 km2). At the present time only A. hickmani is listed as Rare under Tasmanian (State) threatened species legislation but A. helonomus merits the same listing under State legislation. Global warming appears to pose the most significant potential threat to Allanaspides species.

New records in Tanzania of the Vulnerable Jackson's mongoose Bdeogale jacksoni expand its distribution by over 900 km to the south. During two independent camera-trap surveys over 3 years B. jacksoni, one of Africa's most cryptic small carnivores and previously thought to be endemic to Kenya, was recorded in forests of the Udzungwa Mountains. All records were highly localized within Matundu forest, at a maximum of 2.65 km apart. Most of the records (73%) were between 19.00 and 00.00, confirming that the species is primarily nocturnal. Conservation recommendations include further ecological research, genetic analyses, surveys in other Eastern Arc and ground-water dependent forests, and greater protection for Matundu.

Bushmeat is a major food and economic resource in sub-Saharan Africa, and with increasing urbanization bushmeat consumption has spread from villages to cities. To facilitate long-term monitoring of the bushmeat market in Yaoundé, Cameroon, we carried out a baseline survey of the transportation routes of bushmeat into the city and of urban bushmeat sales. We inventoried all bushmeat sales points, and located 15 markets and 145 restaurants and cafeterias selling an estimated total of 1,052 bushmeat dishes per day. This trade provides an occupation for 249 people, of whom 84.3% are women. Trains from Ngaoundere in the north and minibuses from the east supply more than 70% of the sales points in the Elig-Edzoa market, located next to the railway, and the Nkoldongo bus station. From these two places bushmeat is redistributed to other commercial sites within the city. The routes into Yaoundé transport bushmeat from a vast area of the country and in particular from the savannah and central provinces, which are rich in wildlife and contain National Parks and safari hunting areas. However, overall, bushmeat consumption at commercial outlets in the city is low relative to the number of inhabitants.