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130 kda polypeptide

International Journal of Tropical Insect Science (1)

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1 results

Integrity of the 130 kda polypeptide of Bacillus Thuringiensis SSP. Israelensis δ-Endotoxin in K-S sporulation medium
Z. Ngalo Otieno-Ayayo, David M. Chipman, Arieh Zaritsky, Kamal Khawaled
Journal:

Insect Science and Its Application / Volume 14 / Issue 3 / June 1993

Published online by Cambridge University Press:

19 September 2011, pp. 377-381

Print publication:

June 1993
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The 130 kDa polypeptide appears to be the most susceptible of the polypeptides constituting the Bacillus thuringiensis ssp. israelensis δ-endotoxin to the proteases associated with sporulation. Stabilization of this polypeptide in the recently described K-S sporulation medium has been achieved through limiting its cleavage by removing contaminating proteases from the culture by washing. In this way, the complete δ-endotoxin profile is retained for studies on all of the constituent polypeptide fractions.