Whole-grain highland hull-less barley (WHLB) contains high amounts of bioactive compounds that potentially exhibit cholesterol-lowering effects. This study investigated the hypocholesterolaemic effect of WHLB. A total of seventy-two male Sprague–Dawley rats were divided into four groups and were fed with the normal control diet, high-fat diet (HFD) and HFD containing low or high dose (10 or 48·95 %) of WHLB. High dose of WHLB significantly decreased the organ indexes of liver and abdominal fat and lipid levels of plasma and liver in HFD rats. The lipid regulation effect of WHLB, which was reconfirmed through hepatocyte morphologic observation, was accompanied by a large excretion of bile acids in the small intestinal contents and the faeces. Real-time PCR analyses, which were further reconfirmed through Western blot analyses, revealed that a high dose of WHLB significantly enhanced the hepatic expressions of AMP-activated protein kinase α, cholesterol 7α-hydroxylase, LDL receptor, liver X receptor, and PPARα and decreased the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase. It also enhanced the ileal expression of farnesoid X receptor and resulted in the decrease of expression of apical sodium-dependent bile acid transporter. WHLB exhibited hypocholesterolaemic effects mainly by inhibiting cholesterol synthesis, cholesterol accumulation in peripheral tissue, and bile acid reabsorption and by stimulating bile acid synthesis.

Field experiments were conducted at Vegreville and Lacombe, AB, to determine the influence of barley (Hordeum vulgare) variety and seeding rate on interference of wild oat (Avena fatua) with barley. Barley variety and seeding rate affected barley density, height at maturity, and seed yield, as well as wild oat shoot dry weight and seed yield in most experiments, but there was no variety by seeding rate interaction. As expected, the semidwarf varieties Falcon and CDC Earl were the shortest. Barley seedling emergence and subsequent plant densities varied among varieties, locations, and years. The hull-less varieties Falcon and CDC Dawn had the poorest emergence in most cases, whereas AC Lacombe and Seebe had the highest emergence. Wild oat shoot dry matter and seed production was highest in the Falcon, CDC Dawn, and CDC Earl plots, suggesting that these were the least competitive with wild oat. Barley yield loss from wild oat interference also tended to be highest in these varieties. Poor emergence of Falcon and CDC Dawn and the shorter stature of Falcon and CDC Earl likely contributed to their relatively poor competitiveness with wild oat. Increasing the seeding rate improved the competitiveness of all varieties, as evidenced by reduced wild oat shoot dry matter and seed production and, in some cases, improved barley yields.

Field experiments were conducted at Vegreville, Alberta, in 1997, 1998, and 1999 and in Lacombe, Alberta, in 1997 and 1998, to determine if barley row spacing (20 and 30 cm) and seeding rate (75, 125, and 175 kg ha−1) influenced the effects of variable tralkoxydim rates on barley seed yield, net economic returns, and wild oat seed production. In most cases, barley seed yield was unaffected by row spacing or seeding rate. Where no herbicide was applied, the presence of wild oat reduced barley yield at each location each year. When the herbicide was applied at 50, 75, or 100% of the recommended rate, barley yields were not affected by the presence of wild oat. Results were more variable at 25% of the recommended rate, especially at Lacombe, where yield losses occurred both years at this rate. The lowest net economic returns consistently occurred in the absence of herbicide application; however, the influence of herbicide rate on net returns varied among years and locations. Net returns were either higher at the lower herbicide rates or were unaffected by herbicide rate. Seeding rate and herbicide rate affected wild oat seed production at each location each year and also the amount of seeds in the soil seedbank at Vegreville in 1999. Row spacing had little or no effect on wild oat seed production. There was a consistent and highly significant seeding rate by herbicide rate interaction on wild oat seed production. The effects of tralkoxydim on wild oat seed production, especially at relatively low rates, were superior at the higher barley seeding rates. The results suggest that seeding barley at relatively high rates can result in optimum barley yields, undiminished economic returns, and effective wild oat management when tralkoxydim is used at lower than recommended rates.