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This chapter delves into the crucial step of biomass pretreatment and its significance in a biorefinery. It begins by introducing a comprehensive definition of a biorefinery and the importance of pretreatment in biorefining. Various pretreatment methods, their advantages, disadvantages, and accompanying structural modifications to the biomass are explained. The general focus is on the impact of pretreatment on enzyme hydrolysis, an essential step in biomass conversion to renewable sugars for producing various bioproducts, including biofuels and biopolymers such as bioplastics. The chapter further discusses how pretreatments, if not balanced, could also contribute to downstream processing challenges, such as the generation of inhibitors. The chapter provides a comprehensive guide to grasping the necessity of pretreatment in biomass utilization for sustainable biorefining.
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