A rainfall simulator was used to deliver the equivalent of 1.3 cm of water in 0.25 h at 0, 3, 6, 12, 24, and 48 h after POST applications of asulam to johnsongrass in greenhouse and field studies. Johnsongrass control responses were similar when asulam was applied with either a crop oil concentrate or a commercially blended organosilicone/crop oil concentrate premix. Rainfall 24 h after asulam application reduced johnsongrass control in greenhouse studies. Maximum visual johnsongrass control of 80, 69, and 69% was obtained in field studies when rainfall occurred 20, 14, and 8 h after asulam application, respectively. Based on reductions in johnsongrass dry weight, rainfree periods needed to insure maximum performance with asulam in field studies ranged from 3 to 16 h. Variation in critical rainfree periods was related to plant growth stage when asulam was applied and environmental conditions and is indicative of the inconsistency in johnsongrass control commonly observed with asulam.

Until recently, phytopathogenic bacteria have not been considered potential biological weed control candidates because they lack the ability to penetrate intact plants. This deficiency can be overcome by providing entry wounds or using surfactants. Spray application of Pseudomonas syringae pv. tagetis (5 × 108cells/ml) in aqueous buffer with a surfactant produced severe disease in Canada thistle, common ragweed, Jerusalem artichoke, sunflower, and certain other members of the Compositae under field conditions. Spray application of the bacterium without surfactant was ineffective on all reported hosts. Xanthomonas campestris pv. poannua controlled annual bluegrass in bermudagrass golf greens when applied by spray during mowing. The bacterium entered through mowing injuries, causing lethal, systemic wilt. Application of the bacterium to annual bluegrass in the absence of fresh mowing injuries failed to produce symptoms. Under field conditions, this previously unknown pathovar's host range was limited to a single subspecies of annual bluegrass, but inundative application to freshly mowed turf resulted in infection of diverse annual bluegrass biotypes. In field trials, six monthly applications resulted in greater than 70% control. The preceding examples are among the first attempts to use foliar phytopathogenic bacteria for biological weed control. Efficacy of these bacterial bioherbicides and of future biocontrol strategies employing bacteria is dependent on facilitated host penetration.

The relationship between surface activity and weed control efficacy of diuron spray solution (0.075 kg ai/ha) with 12 adjuvants (0.1% v/v) was studied in the laboratory and the greenhouse. Organosilicone adjuvants reduced surface tension (ST) and contact angle (CA) of diuron spray solutions to a greater extent than did nonsilicone adjuvants. Three organosilicone adjuvants significantly increased the control and reduced the fresh weight of barnyardgrass. Contact angle was more closely correlated with diuron efficacy than ST, and the coefficient of determination between the CA and the fresh weight or control percent of barnyardgrass was 0.41. Kinetic® applied before or in combination with diuron significantly enhanced diuron efficacy. However, Kinetic sprayed after diuron did not affect the efficacy. Kinetic influenced the retention and absorption of diuron rather than its deposition and translocation.