Relational turbulence theory (RTT) proposes causal relationships across cognitive, emotional and communicative variables. Although many tenets of this theory have been tested individually, there has not yet been a comprehensive, predictive examination of RTT. Using structural equation modelling, this study longitudinally tested several propositions and axioms of RTT. Results are largely in line with many of RTT's predictions. Time 1 relational uncertainty predicted time 2 biased cognitions. Time 1 facilitation from a partner predicted time 2 negative emotions. Negative emotions cross-sectionally related to both the enactment and valence of relational communication episodes. Relational turbulence theory's proposed feedback loop received partial support, such that time 1 communication valence (but not engagement) predicted time 2 partner uncertainty, relationship uncertainty and partner facilitation. Results are discussed in terms of theory expansion and refinement.