In Mississippi, rice reproduction and ripening often overlaps with soybean maturation, creating potential for herbicide exposure onto rice from desiccants applied to soybeans. Six independent studies were conducted concurrently at the Delta Research and Extension Center in Stoneville, MS, from 2016 to 2018 to determine the response of rice to sublethal concentrations of soybean desiccants during rice reproductive and ripening growth stages. Studies included the desiccants paraquat, glyphosate, saflufenacil, sodium chlorate, paraquat + saflufenacil, and paraquat + sodium chlorate applied at a rate equal to 1/10th of Mississippi recommendations. Treatments were applied at five different rice growth stages, beginning at 50% heading––defined as 0 d after heading (DAH)––with subsequent applications at 1-wk intervals (0, 7, 14, 21, and 28 DAH), up to harvest. Injury was observed 7 d after application (DAA), with five of six desiccants at all application timings. No injury was observed with glyphosate application across all rating intervals. Rough rice grain yield following all glyphosate applications was reduced by >6%. In the studies evaluating paraquat, injury ranged from 5% to 18% at all evaluations, regardless of application timing. Rough rice grain yield was reduced >12% 0 to 21 DAH, following paraquat application. Similar trends were observed with paraquat + saflufenacil and paraquat + sodium chlorate, with rice exhibiting yield decreases >6% following an application 0 to 14 and 0 to 21 DAH, respectively. In studies evaluating saflufenacil and sodium chlorate, rough rice grain yield was >95% of the untreated across all application timings Yield component trends closely resembled reductions observed in rough rice grain yield. Reductions in head rice yield were >5% following applications of paraquat or paraquat + saflufenacil 0 to 14 and 0 to 21 DAH, respectively. Late-season exposure to sublethal concentrations of desiccant from 50% heading (0 DAH) to 28 DAH has an impact on rough rice grain yield, yield components, and head rice yield.

In situ liquid cell transmission electron microscopy (LC-TEM) is an innovative method for studying the processes involved in the formation of crystals in liquids. However, it is difficult to capture early stages of crystallization because of the small field of view and the unfavorable changes in sample composition resulting from electron-beam radiolysis. Nevertheless, if the radiolysis required to induce the crystallization of a sample could be controlled in LC-TEM, this would be advantageous for observing the crystallization process. Here, we examined this possibility by using a mixture of sodium chlorate (NaClO3) and acetone in the LC-TEM. The electron beam induced the formation of dendritic crystals in a saturated acetone solution of NaClO3; moreover, these crystals consisted of sodium chloride (NaCl), rather than NaClO3, suggesting that chloride ions (Cl−), which were not present in the initial solution, were generated by radiolysis of chlorate ions ${\rm ( ClO}_3^- )$. As a result, the solution then supersaturated with NaCl because its solubility in acetone is much lower than that of NaClO3. The combination of radiolysis and a solvent in which a solute is much less soluble is potentially useful for establishing crystallization conditions for materials that are difficult to crystallize directly in LC-TEM experiments.

Field experiments were conducted at three locations in Mississippi in 1995 and 1996 to evaluate labeled and alternative herbicides and herbicide combinations for weed desiccation prior to soybean harvest. Weeds evaluated included pitted morningglory, hemp sesbania, spotted spurge, common cocklebur, and sicklepod. Soybean yield and harvestable soybean losses were not affected by preharvest herbicide treatments, spray volume, or surfactant concentration. Soybean moisture was most consistently reduced by glufosinate compared to the untreated and other herbicides and herbicide combinations evaluated. Most desiccation treatments at Stoneville and Brooksville resulted in foreign material similar to the weed-free check. Glufosinate at 0.84, 1.1, and 1.4 kg ai/ha desiccated all weeds evaluated 90% or more with no differences among rates. The addition of 3.4 or 6.7 kg ai/ha sodium chlorate to 0.28 kg ai/ha paraquat, 1.1 and 2.2 kg ai/ha glyphosate, or 0.28 and 0.56 kg ai/ha oxyfluorfen increased desiccation of most weeds evaluated, with no difference between sodium chlorate rates. In some instances, reducing application volume from 281 to 94 L/ha improved pitted morningglory desiccation when 0.28 g/ha paraquat was applied alone. There were no differences between 0.25 and 0.50% (v/v) surfactant for most parameters evaluated.

Sicklepod (Senna obtusifolia), hemp sesbania (Sesbania exaltata), and pitted morningglory (Ipomoea lacunosa) seed production and viability in early-maturing soybean (Glycine max) were evaluated following preharvest desiccation. The herbicide combinations 1.1 kg ai/ha glyphosate plus 6.7 kg ai/ha sodium chlorate, 2.2 kg/ha glyphosate plus 3.4 kg/ha sodium chlorate, and 0.3 kg ai/ha paraquat plus 6.7 kg/ha sodium chlorate reduced sicklepod germination, emergence, and seedling growth in most cases. Oxyfluorfen at 0.3 kg ai/ha plus 3.4 or 6.7 kg/ha sodium chlorate also reduced sicklepod germination and growth. Glufosinate from 0.8 to 1.4 kg ai/ha reduced sicklepod germination but did not have a consistent effect on other seedling growth parameters. Bromoxynil at 1.1 kg ai/ha plus 3.4 kg/ha sodium chlorate reduced sicklepod germination but did not have a consistent effect on most growth parameters. A wide range of treatments reduced hemp sesbania germination, emergence, and growth to very low levels. All desiccant applications reduced pitted morningglory seed production compared to the untreated check. Glyphosate at 1.1 kg/ha plus 6.7 kg/ha sodium chlorate, 0.3 kg/ha oxyfluorfen plus 3.4 kg/ha sodium chlorate, and 0.8 or 1.1 kg/ha glufosinate reduced pitted morningglory seed production and desiccated weeds effectively. Most treatments reduced emergence in 1996, when seeds were smaller and less mature than those collected in 1995. When application volume of paraquat alone or in tank mixture decreased, germination and growth of sicklepod was reduced, indicating increased paraquat efficacy.

Research was conducted over 2 yr to evaluate soybean response to harvest aid herbicide treatments paraquat at 0.28 kg ai ha−1, paraquat with carfentrazone at 0.014 kg ai ha−1, and sodium chlorate at 6.72 kg ai ha−1. Indeterminate and determinate soybean cultivars were treated when moisture of seed collected from the uppermost four nodes of plants averaged 60, 50, 40, 30, and 20% (± 2%). For each soybean cultivar, the harvest aid treatment by application timing interaction was not significant, and data for harvest aid treatments were averaged. Application of harvest aid at 60% average seed moisture reduced yield for the maturity group (MG) IV indeterminate cultivar 15.4% compared with the nontreated; 100-seed weight was reduced 12.4%. Yield and seed weight were not negatively affected when harvest aid was applied at 50% average seed moisture and soybean was harvested 14 and 15 d before the nontreated control. Although planting date in the 2 yr for the indeterminate cultivar differed by 26 d, number of days from planting to harvest aid application at 50% average seed moisture was 112 and 116 d. For MG V and MG VI determinate cultivars, application of harvest aid at 60% average seed moisture reduced yield compared with the nontreated control 22 and 18.1%, respectively, and at 50% average seed moisture 15.6 and 4%, respectively; seed weight reductions of 8.9 to 33.3% accompanied the yield reductions of the two cultivars. Reduction in soybean yield and seed weight was not observed when harvest aid was applied at 40% average seed moisture, and harvest for the 2 yr was 8 and 9 d earlier for the MG V cultivar and 10 and 14 d earlier for the MG VI cultivar.

Group IV Glycine max cultivars, chlorimuron postemergence, and paraquat + sodium chlorate applied as preharvest desiccants were evaluated for their effect on weed seed production and viability. Early-maturing G. max cultivars reduced seed weight, seed production, and seedling growth in Ipomoea lacunosa in most instances and usually reduced seed weight, germination, emergence, and growth in Sesbania exaltata by allowing harvest prior to physiological maturity of these weeds. Tall, late-maturing G. max decreased weed seed production and seed weight of both species, presumably through increased competitiveness of G. max. The seeds produced by weeds that emerged after chlorimuron application were usually lower in weight, germination, and emergence, and growth of seedlings was reduced because most weeds present at the time of application were controlled. Thus, only late-emerging or stunted plants were present to produce seed. Preharvest desiccation of late-maturing S. exaltata parent plants with paraquat + sodium chlorate reduced height and fresh weight of the resulting S. exaltata seedlings 35 to 50% by reducing the amount of time for the S. exaltata seeds to mature.

The effect of two commonly used desiccant tank mixtures on Glycine max seed production and viability when applied at G. max growth stages R5 to R8 (R5 = beginning pod fill, R6 = full green pod, R7 = beginning maturity, R8 = full maturity) was evaluated. Two mid-Group IV G. max cultivars, one conventional (‘Northrup King 4260’) and one glyphosate-tolerant (‘Asgrow 4701RR’), were evaluated. Tank mixtures containing either glyphosate + sodium chlorate or paraquat + sodium chlorate + surfactant reduced yield, seed weight, and subsequent germination, emergence, and seedling growth when applied at R5 or R6 to either G. max cultivar. Glyphosate + sodium chlorate applied at R7 reduced germination of the glyphosate-tolerant cultivar. When applied at R6 or R7, glyphosate + sodium chlorate reduced the next generation's seedling length compared to paraquat + sodium chlorate. Glyphosate + sodium chlorate increased the number of abnormal seedlings produced in the conventional cultivar compared to paraquat + sodium chlorate. These results indicate that preharvest desiccant applications should not be made prior to G. max maturity (R7).