Systemic uptake of organic compounds from roots to leaves follows a Gaussian distribution in relation to the lipophilicity, as measured by the log Kow. Quantification of compound uptake with different lipophilicities, and applied as a seed treatment that diffuses through the seed coat into the embryo during imbibition, has not been reported. The aim of this investigation was to quantify the uptake of non-ionic compounds into seeds of soybean and corn. A series of fluorescent piperonyl amides were synthesized and a novel combinatorial pharmacodynamic technique was developed that provided a range of compounds from log Kow 0.02 to 5.7. Seeds were treated with a mixture of amides, imbibed and compounds chemically extracted and quantified by high-performance liquid chromatography using a fluorescence detector. The maximum uptake efficiency of the applied amide mixture from whole soybean and corn seeds was 67% at log Kow 2.9, and 43% at log Kow 3.4, respectively. The critical partition coefficient for uptake for both species was <4.2 log Kow. Seeds were dissected and separated as soybean embryo and testa, and corn internal tissues (embryo + endosperm) or seed covering layers (pericarp + testa), and >75% of the amides were found in the soybean embryo or corn internal tissues compared with the covering layers at log Kow <4.2. The distribution of amides showed that the corn seed covering layer had similar hydrophilic/lipophilic properties as internal tissues, while soybean tissues had different hydrophilic/lipophilic properties. Collectively, the Gaussian uptake pattern for systemic uptake into plants was not found for either seed species.