The Chinese pangolin Manis pentadactyla is categorized as Critically Endangered on the IUCN Red List but little is known about its status in Nepal. Although indirect sign surveys have reported its presence in several community forests in Kavrepalanchok district, no photographic or video evidence has previously been documented. We used camera traps to investigate the occurrence of pangolins in 20 community forests in Panauti Municipality in Kavrepalanchok. A total of 75 0.01 km2 plots were surveyed using camera traps during September 2022–February 2023, with a total survey effort of 803 trap-days. The cameras recorded a total of 16 individual video footage events of Chinese pangolins in six of the community forests. This is the first camera-trap evidence of the species' presence in these forest patches, and in Kavrepalanchok district. The pangolins displayed behaviours such as sniffing, gathering plant material and digging, between 18.00 and 1.00. The camera-trap records provide more accurate species identification and reliable information than indirect sign surveys, indicating camera traps are a useful surveying tool for rare, nocturnal and elusive pangolins.

In Singapore, the Critically Endangered Sunda pangolin Manis javanica is threatened by habitat loss and fragmentation, and road traffic collisions. To mitigate these threats, an understanding of its spatiotemporal distribution is needed, as identified in the National Conservation Strategy and Action Plan for the species. However, Sunda pangolin occurrence data are held in multiple separate databases, are typically collected using non-standardized methods, and often lack accurate location details. To compile a complete georeferenced database of Sunda pangolin records in Singapore, we consolidated occurrence data from heterogeneous databases and mainstream and social media, and converted locality descriptions into geographical coordinates. We demonstrate the use of this database to analyse data on rescued pangolins and those killed on roads, to aid conservation efforts in Singapore, and describe other potential applications. We georeferenced 482 records of pangolin sightings, rescues and roadkill for 1996–2021, finding an increase in all three over the study period. Roadkill and rescues occurred mostly in central and western Singapore, close to forested areas, and were predominantly of subadults and adult males. The data can be used to inform threat mitigation strategies, post-rescue release plans and further research. The database has already been used in practice, contributing to environmental impact assessments and conservation recommendations. Overall, this georeferenced database demonstrates the value of citizen science and collating wildlife data from multiple sources, and the methods used can be applied to other taxa to aid conservation strategies.

A recent study published in Oryx proposed that the extinct Javan tiger Panthera tigris sondaica may still survive on the Island of Java, Indonesia, based on mitochondrial DNA analysis of a single hair sample collected from a location where a tiger was reportedly encountered. However, upon reanalysing the genetic data presented in that study, we conclude that there is little support for this claim. The sequences of the putative tiger hair and Javan tiger museum specimens generated are not from tiger cytoplasmic mitochondrial DNA but more likely the nuclear pseudogene copies of mitochondrial DNA. In addition, the number of mismatches between the two Javan tiger sequences is unusually high for homologous sequences that are both from tigers, suggesting potential issues with data reliability. The paper provides insufficient details on quality control measures, making it impossible to rule out the possibility that errors were introduced during the analysis. Consequently, it is inappropriate to use the sequences presented in that study to infer the existence of the Javan tiger.

Big cats are of conservation concern throughout their range, and genetic tools are often employed to study them for various purposes. However, there are several difficulties in using genetic tools for big cat conservation that could be resolved by modern methods of DNA sequencing. Recent reports of the sighting of a putative Javan tiger Panthera tigris sondaica in West Java, Indonesia, highlight some of the difficulties of studying the genetics of big cats. We reanalysed the data of the original reports and found that the conclusions were drawn based on incorrect copies of the genes. Specifically, the nuclear copy of the mitochondrial gene was analysed with the mitochondrial sequence, leading to discordance in the results. However, re-sequencing of the remaining DNA confirms that the sighting could have been that of a tiger, but the subspecies cannot be confirmed. This work highlights the urgency of developing high-throughput sequencing infrastructure in the tropics and the need for reliable databases for the study of big cats.

The conservation sector increasingly values reflexivity, in which professionals critically reflect on the social, institutional and political aspects of their work. Reflexivity offers diverse benefits, from enhancing individual performance to driving institutional transformation. However, integrating reflexivity into conservation practice remains challenging and is often confined to informal reflections with limited impact. To overcome this challenge, we introduce co-reflexivity, offering an alternative to the binary distinction between social science on or for conservation, which respectively produce critical outsider accounts of conservation or provide social science instruments for achieving conservation objectives. Instead, co-reflexivity is a form of social science with conservation, in which conservation professionals and social scientists jointly develop critical yet constructive perspectives on and approaches to conservation. We demonstrate the value of co-reflexivity by presenting a set of reflections on the project model, the dominant framework for conservation funding, which organizes conservation activity into distinct, target-oriented and temporally bounded units that can be funded, implemented and evaluated separately. Co-reflexivity helps reveal the diverse challenges that the project model creates for conservation practice, including for the adoption of reflexivity itself. Putting insights from social science research in dialogue with reflections from conservation professionals, we co-produce a critique of project-based conservation with both theoretical and practical implications. These cross-disciplinary conversations provide a case study of how co-reflexivity can enhance the conservation–social science relationship.

The regent honeyeater Anthochaera phrygia is a Critically Endangered Australian songbird, with current population estimates of < 300 individuals remaining in the wild. Low nest success is a factor preventing the recovery of the population, and management remedies are needed. However, a lack of data on intervention success raises uncertainty and impedes planning. To identify management priorities under uncertainty, we engaged with conservation practitioners and key stakeholders to develop and evaluate potential nest protection interventions. Four categories of threats were considered: avian predators, mammalian predators, extreme weather events and avian competitors. The interventions with the highest predicted probabilities of nest success under each threat category were, respectively: lethal control of avian predators, the use of tree collars to control arboreal mammalian predators, the provisioning of supplementary food and nesting resources during extreme weather events, and control of the noisy miner Manorina melanocephala, a competitor species. Our analysis shows that by applying a combination of conservation actions alongside improvements in nest detection, it is possible, based on the opinion of experts, to provide a pathway for the recovery of the regent honeyeater.

Human–wildlife conflict is a critical and complex challenge in wildlife conservation. It arises when humans and wildlife interact and one or both parties suffer negative consequences from the interaction. This research assessed the extent of damage resulting from human–African buffalo Syncerus caffer conflict and explored mitigation strategies. We used a semi-structured questionnaire-based survey of 131 households randomly selected in Kambi ya Simba, Oldeani and Tloma villages surrounding Ngorongoro Conservation Area, Tanzania. Our results revealed that resource competition was the main factor inducing human–African buffalo conflict. The impacts reported most frequently were crop damage (especially during the wet seasons) and human casualties. Crops that were most often damaged included maize, beans, wheat, peas and coffee. To minimize buffalo crop depredation, farmers currently use traditional mitigation approaches such as guarding farms, lighting fires, using torchlight and vocal and other auditory deterrents. In addition, a local coffee estate installed electrified fencing around its plantation. Our findings demonstrate the impacts of human–African buffalo conflict on local communities and the importance of continuing human–African buffalo conflict monitoring to improve conservation action and increase the participation of the local community in conservation activities. To minimize human–African buffalo conflict, we recommend conservation strategies that improve the natural habitat of the African buffalo. Most importantly, providing communities affected by human–African buffalo conflict with modern and more effective mitigation methods, paired with increased community awareness of the use of these methods, could result in significant reductions in the human cost of human–African buffalo conflict.

Mouse lemurs Microcebus spp. are small, nocturnal primates endemic to Madagascar. The genus is extraordinarily diverse, with 25 extant species, several of which have been described recently. The Endangered Claire's mouse lemur Microcebus mamiratra was first described in 2006, but, similarly to other newly described mouse lemurs, remains understudied, and estimates of its population size are unavailable, hampering effective conservation management. We conducted line transect distance sampling surveys of M. mamiratra across several habitat types in and around Lokobe National Park on the island of Nosy Be in north-western Madagascar. Using a systematic random design we surveyed 15 transects over a 6-week period in 2023, recording 92 detections from a total survey effort of 46.5 km. We estimate the density of M. mamiratra on Nosy Be to be 125.1 individuals/km2, which extrapolates to an estimate of c. 4,700 individuals across the forested areas of its range on the island. Our results indicate that Nosy Be harbours moderately high densities of M. mamiratra, with the highest encounter rates in the unprotected secondary and degraded forests around Lokobe National Park. Our population estimate will inform future conservation status assessments and conservation planning for this range-restricted species and provide a baseline for monitoring population changes over time. We present recommendations for the conservation of M. mamiratra and highlight the potential for lemur watching, sustained by the strong tourism industry on Nosy Be, to help protect lemur habitat and generate economic opportunities for local communities.

The rare Himalayan wolf Canis lupus chanco is categorized as Vulnerable on the IUCN Red List, and there is limited knowledge of its ecology and distribution. In Bhutan, the Himalayan wolf is one of the least known carnivores. Our aims in this study were to map the current distribution of the wolf in Bhutan and to identify potential habitats within the country. We compiled 32 records of wolf presence from camera-trap surveys and, using a maximum entropy approach, we estimated a potential habitat of 2,431 km2, comprising c. 6.3% of Bhutan. However, wolf presence was localized and non-continuous. We recommend a detailed fine-scale habitat analysis in areas of potential habitat and genetic studies to investigate population structure. Knowledge of these matters will provide insights regarding connectivity and facilitate the development of conservation strategies for this threatened wolf.

The record of mammal declines and extinctions in Australia raises concerns regarding geographically restricted and poorly known taxa. For many taxa, the existing data are insufficient to assess their conservation status and inform appropriate management. Concerns regarding the persistence of the subspecies of yellow-footed rock-wallaby Petrogale xanthopus celeris, which is endemic to Queensland, have been expressed since the 1970s because of red fox Vulpes vulpes predation, competition with feral goats Capra hircus and land clearing. This rock-wallaby is rarely observed, occupies rugged mountain ranges and, prior to our surveys, had not been surveyed for 25 years. We surveyed 138 sites across the range of this rock-wallaby during 2010–2023, including revisiting sites surveyed in the 1970s–1980s and locations of historical records. We examined occurrence in relation to habitat variables and threats. Occupancy and abundance remained similar over time at most sites. However, by 2023 the subspecies had recolonized areas in the north-east of its range where it had disappeared between surveys in the 1980s and 2010s, and three south-western subpopulations that were considered extinct in the 1980s were rediscovered. Recolonization and increases in abundance at numerous sites between the 2010s and 2020s are associated with declines in feral goat abundance, indicating dietary and habitat competition are major threats. Exclusion fences erected since 2010 could limit genetic exchange between rock-wallaby subpopulations whilst allowing domestic goats to be commercially grazed. Petrogale xanthopus celeris should remain categorized as Vulnerable based on these ongoing threats. Repeated monitoring approximately every decade should underpin management of this endemic taxon.