Reporting of outcome variables by caregivers in welfare studies is commonplace but is open to subjective bias and so requires validation. Biases can occur in either direction: familiarity with an animal allows a deeper insight into welfare problems, but also can lead to reticence in admitting that an animal in one's care is experiencing problems. Here, we aim to validate owner-reporting of plumage condition of pet parrots, including those with self-inflicted feather-damaging behaviour (FDB), by comparing owners’ scores of feather condition with those of two independent raters, blind to the owners’ and each other's assessments. We surveyed pet parrot owners to collect data on basic demographics and feather condition, and requested four standardised photographs of birds. We received 259 responses (17% of the 1,521 people contacted); 78 sets of images of appropriate quality for assessment by raters were provided. Mean percentage agreement between owners’ and raters’ scores was mostly fair to substantial using Cohen's kappa; however, raters scored a greater proportion of feather damage than did owners. Overall, our results indicate owner-reporting of feather condition, including FDB, to be generally reliable and consistent with independent assessment of photographs. As the use of photographs can be limited by image quality, a failure to represent the long-term state of a parrot, and the potential for incorrect recording if assessed without relevant information (eg on moulting), this evidence that owner-reports can be reliable opens the door for larger-scale surveys of the extent of welfare-relevant problems.

There are instances when animals are introduced and expected to live alongside unfamiliar conspecifics within zoos, laboratories and wildlife sanctuaries. These pairings of unfamiliar animals may result in stress, trauma, or even death, in addition to reduced confidence in data resulting from these subjects. For species that communicate relatedness, sex, social status, and emotional state through olfactory cues (eg pheromones), one means of counteracting aggression may involve a period of partial separation — where animals are close enough to become acquainted — while a permeable barrier maintains separation. For our study, we evaluated the use of a novel, autoclavable, wire-mesh partition to separate potential aggressors. We tested different pairs of 24 wild-type male Norway rats (Rattus norvegicus), previously kept in social isolation for seven days. Each control pair were merged directly into one cage, while pairs from the experimental groups underwent three pre-exposure sessions that lasted two to four days. We used continuous video recordings to assess five common threat displays: lateral threat, keep down, upright posture, chase, and clinch attack. We used two types of bedding: new (unscented) bedding and recently used bedding that conveyed scents from both merged rats. We found that rats subjected to pre-exposure demonstrated lower aggression levels across three of the five metrics (lateral threats, upright postures, and keep downs). We conclude that permeable partitions show promise as a humane mechanism to mix new individuals into preexisting colonies. Further research may explore whether partitions could be helpful with other species that communicate social information by pheromones or direct visual inspection.

Slaughter is considered an important fish welfare issue. For a slaughter method to be considered humane, effective stunning that lasts until death as well as the reduction of pain and fear throughout all procedures are essential. Our objective was to investigate current techniques for slaughtering fish in Brazil. A digital questionnaire with six multiple-choice and three open questions was distributed to companies registered as fish slaughterhouses and to fish farms in 2019, addressing fish species, quantities processed and slaughter procedures adopted. From 62 facilities in 15 Brazilian states that answered the survey, nine slaughterhouses and 30 fish farms slaughtered fish, totalling 452 tonnes per day. All reported pre-slaughter stunning, and live chilling to be the most commonly cited method (82.0%), followed by electronarcosis (18.0%). Slaughtering techniques included exsanguination (38.5%) and decapitation (2.5%). For the remaining companies, no slaughter method was declared, suggesting death by asphyxiation or by further processing (59.0%). Twenty-nine companies adopted pre-slaughter fasting, lasting from 10 to 48 h. All sites worked with tilapia (100%) and 24 (82.7%) reported that they worked exclusively with this species. Other species reported were: pacu (25.6%), tambacu (17.9%), tambaqui and carp (15.3%), jundiá (12.8%), pintado and pirarucu (7.7%), matrinxã and pangassius (5.1%). Asphyxia and live chilling are not considered humane, as animals remain conscious and, thus, suffer. Results show that most establishments do not perform humane slaughter. This scenario highlights the urgent need for development and enforcement of humane fish slaughter techniques, with routine supervision and normative requirements.

Snake ownership is popular; however, housing and care may not always satisfy the animal's welfare needs. For example, snakes are often kept in environmental conditions that restrict their ability to stretch out fully or move around, using rectilinear locomotion. To date, little empirical data exist on the housing and husbandry of captive snakes and how these impact welfare. This study analyses survey responses from 744 snake owners worldwide and explores potential welfare concerns for snakes kept within private homes. It documents the most common housing and husbandry methods and compares the three most common families (Pythonids, Boids and Colubrids). Owner-reported data were used to derive scores for two potential welfare indicators: number of clinical signs of ill health and the number of reported negative (abnormal) behaviours. Using these indicators, associations were tested with snake-keeping variables, such as enclosure size, level of enrichment, temperature and humidity. Owners reported that 90.7% (n = 675) of snakes adopted rectilinear (straight-line or near straight-line) positions or movements. Snakes kept in enclosures > 1 snake length tended to have more enrichment types and were reported with fewer clinical signs of ill health. Some snakes were housed at unknown temperature (7.7%) or humidity (48.1%) ranges or in environmental conditions not in keeping with current recommended guidelines. Corn snakes (Pantherophis guttatus) were the most likely species to be kept at a humidity which did not match recommendations which may present a welfare concern. This study's results can be used to target husbandry guidance for future recommendations and care manuals.

Breeding and housing wild animals in captive environments can pose challenges for their welfare. In South Africa, thousands of lions (Panthera leo) are bred and raised at commercial captive breeding facilities, so called ‘lion farms’, for use in tourism, trophy hunting and traditional medicine. To gain a better understanding of the potential welfare challenges faced by lions on farms we reviewed 91 peer-reviewed articles relating to lion welfare, identified via a systematic review of the scientific literature. Across these studies, we identified 170 different terms relating to negative behaviours and physical health afflictions. The majority of these terms were associated with disease and injury (124; 73%), followed by negative behaviours (19; 11%), negative mental experiences (15; 9%), nutritional concerns (7; 4%), and environmental challenges or discomfort arising from the animal's surroundings (5; 3%). Of the 91 articles, 32 (35%) focused on data concerning captive lions. Only two studies focused specifically on data obtained from lion farms in South Africa, whilst the remainder reported on data collected from zoos, wildlife parks, sanctuaries, game reserves and private ownership. Our preliminary review of the scientific literature draws attention to some of the challenges associated with caring for lions in captivity, and outlines the potential significance of these welfare challenges for commercial lion farms. Our data highlight the apparent lack of scientific research involving captive lion welfare generally, particularly data collected at commercial breeding facilities in South Africa and the consequences this could have for the welfare of thousands of lions within the industry.

For animals undergoing rehabilitation it is vital to monitor welfare in a way that is feasible, practical, and limits stress to the animal. The industry gold standard is to assess welfare under the Five Domains model, including nutrition, environment, physical health, and behaviour as the first four physical domains and mental domain as the fifth. Feasibility and effectiveness of these domains for assessing welfare of sea turtles undergoing rehabilitation were reviewed and it was determined that the mental state can be best assessed through behavioural changes. A scoping review of the literature was conducted using Scopus and Web of Science to investigate use of environmental enrichment devices (EEDs) as a measure of welfare in sea turtles. Behavioural assessments using EEDs were found to be well-documented; however, most EED studies pertained largely to livestock or zoo animals. Furthermore, studies rarely concentrated on reptiles, and specifically sea turtles. Results also showed that certain welfare assessment methods may be less appropriate for short-term captivity experienced during rehabilitation. Additionally, the hospital environment limits the ability to address some of the domains (ie biosecurity, feasibility, safety of turtle, etc, might be compromised). This review shows that only three of the nine environmental enrichment strategies described in the literature suit the specific requirements of sea turtles in rehabilitation: feeding, tactile, and structural. It is documented that turtles display behaviours that would benefit from EEDs and, therefore, more specific studies are needed to ensure the best welfare outcomes for sea turtles undergoing rehabilitation.

Fixation of the tongue to the mandible using so-called tongue ties (TTs) is common practice in Standardbred (SB) and Thoroughbred (TB) racing, but little is known about their impact on animal welfare. In this study, the influence of TTs on heart-rate variability (HRV), stress parameters in plasma (cortisol, glucose, lactate) and behaviour was evaluated in 30 SBs and 29 TBs (n = 59) presenting with exercise insufficiency. Overall, 36/59 horses (24 SBs, 12 TBs) were familiar with TTs. Blood was taken at rest, after TT application and after racing in all horses, additionally samples were taken without TT in SBs another day. HRV was calculated over 3 min before, during and after racing. Additionally, SBs’ behaviour during TT application and racing was documented in real time. TT application did not increase cortisol levels significantly, while highly significant increases in cortisol levels were found after racing. Lactate levels were not influenced by TT application, but also significantly increased after racing. No significant differences were found for glucose. Seventeen out of 30 SBs showed mild (n = 8), moderate (n = 8) and severe (n = 1) reactions during TT application, none during or after race training. At rest, 23/30 SBs had a low/high frequency (LF/HF) ratio < 1.5 (1.05 [± 0.61], n = 30, dominating parasympathetic activity). After TT application, the LF/HF ratio increased to 1.4 (± 0.45) (increased sympathetic activity). In TBs, sympathetic activity dominated at rest. No differences in LF, HF and LF/HF were found after TT application or comparing HRV after racing with/without TT. The stress response (blood parameters and HRV) was not influenced by horses’ naivety to TTs, however an increased stress response was observed in SB mares. Overall, obvious adverse behaviour, but only slight evidence of an increased systemic stress response, was found in this study. These results might provide objective evidence for future decisions from equine sports organisations concerning further regulations on TTs.

Developing effective enrichments is important for improving pig (Sus scrofa) welfare as it increases species-specific behaviours, decreases abnormal behaviours, and increases time active. However, few enrichments are available for sows in farrowing crates. Pigs are often observed to scratch, or rub against objects, however enrichments designed to provide a scratching outlet have never been tested in sows. We examined the behaviour and welfare of sows in farrowing crates when they were presented with one of two types of scratchpad enrichment. Sows (n = 18) of parities two (P2) and three (P3) were housed for 25 days and assigned no enrichment (Control) or a scratch-pad made of plastic mats (Plastic) or coir fibre mats (Fibre). Parity two Plastic sows scratched for a longer total duration than P2 and P3 Fibre sows, P3 Plastic sows, and P2 Control sows. Parity two Plastic sows also displayed scratching bouts more frequently than all except P3 Control sows. There were no body lesion differences between treatments. Abnormal behaviour and proportion of time spent in different postures also did not differ between treatments. Plastic scratch-pads may be a suitable enrichment for farrowing crates as they increased the natural behaviour of scratching. More research is needed to refine the scratch-pad design and measure motivation before it can be concluded that scratch-pads are a successful enrichment that should be implemented on-farm.

Previously assumed to be unintelligent animals, many species of birds display high levels of cognition and may even possess conscious awareness. In particular, both corvids and parrots have been the focus of cognitive research including studies on problem-solving, social intelligence, and sentience. Despite their similar neural architecture and cognitive abilities, the laws regarding these two families of birds differ greatly. In the United States, it is illegal to keep corvids as pets. Parrots, however, are one of the most commonly kept pets in America, although their care in captivity remains largely unregulated. Captive parrots suffer from a number of medical and psychological issues and experience high rates of neglect and abandonment. At the same time, wild parrot populations are dwindling due both to habitat loss and capture for the pet trade. This review examines the novel findings on avian cognition and applies them to the potential ethical implications of keeping parrots in captivity. In addition, suggestions for future directions are presented, including the development of legislation to protect captive and wild parrots.

The ethical and economic significance of slaughtering animals for consumption by people of faith cannot be underestimated. On one hand, there are concerns for the welfare of animals during rearing, transport and slaughter, on the other, the market for halal meat products continues to grow at an exponential rate which has attracted the attention of independent and mainstream retailers. This paper considers the slaughter methods approved for the main animal species slaughtered for consumption by Muslims: beef, lamb, goats and poultry. It further examines the rationale for approving and rejecting certain methods of stunning and the implications this has for the welfare of animals. Areas where further research is needed to improve animal welfare during halal slaughter are also highlighted, and the authors have argued why a dialogue between animal welfare researchers, Islamic scholars and halal certification or accreditation bodies is vital in creating knowledge exchange between key stakeholders with a view to improving animal welfare during halal meat production.