The redispersal factor for bacteria-carrying particles from a contaminated floor was determined after mopping, blowing and walking activity. Walking gave the highest redispersal factor, 3·5×10−3 m−1, which was three times higher than for blowing and 17 times higher than for mopping. The mean die-away rate for the bacteria-carrying particles used was 1·9/h without ventilation and 14·3/h with ventilation. It was calculated that in the operating rooms less than 15% of the bacteria found in the air were redispersed floor bacteria.

The single radial haemolysis test is conveniently practical and economical and promises to have wide applicability in the study of influenza antibodies in human populations. It can also be adapted for preliminary examination of new virus isolates during epidemics.

Using this test a rather higher proportion of the population in the Highland Region of Scotland was found to possess antibody to a recent epidemic strain of influenza (A/Scotland/74) than was the case in the south of England. Antibody was detected and apparently evenly spread throughout all but the most remote island communities. Some evidence of the spread of the subsequent variant, A/Victoria/75, was obtained. Most of the school children in our study had high antibody titres to recent strains but the proportion with high antibody titres to these strains declined speedily from the age of 17 years onwards.

Groups of school children aged 6–14 years were tested with PPDS and one of the following five antigens simultaneously, namely PPDA, PPDF, PPDG, PPDPL and PPDY. PPDS was prepared from the human tubercle bacillus, and the others were prepared from M. avium, M. fortuitum, the ‘Gause’ organisms, M. marinum and M. kansasii respectively.

It was observed that there was some increase in induration size to PPDA, PPDG, PPDL, PPDS and PPDY with increase in age, while M. fortuitum gave a preponderance of small reactions with no increase of size with age. It was also observed that there was evidence of cross sensitization between PPDS and all the other antigens, particularly in those who had negative or doubtful reactions.

Clean clothes in the staff dressing rooms were heavily contaminated with bacteria, mainly Bacillus sp., but Staphylococcus aureus were found on 14% and Clostridium sp. on 10% of the garments examined. A comparison of the occurrence of Staph. aureus on shirts worn by staff in wards and operating departments showed ward shirts to be contaminated more heavily and with more strains. Examination of sterile gowns worn by surgeons showed that 70% were contaminated with Staph. aureus after operation. Of the strains isolated 31% were identical with those carried by the surgeon or by the patient operated on, but for the remainder no source could be found.

Haemagglutination inhibition (HI) antibodies against the influenza viruses A/Hong Kong/8/68 (H3N2) and B/Nederland/77/66 were determined in 420 paired sera from mothers and newborns (umbilical cord sera), sampled in 1970–1.

A higher concentration of antibodies against influenza A virus was found more frequently in neonatal than in maternal sera. By contrast, low titres against influenza B virus were more frequently observed in neonatal than in maternal sera. Maternal age, duration of pregnancy, and birth-weight did not affect the results of the tests.

It is suggested that the titre of the newborn against an epidemic influenza virus can be predicted from that of the mother. Furthermore, the maternal titre may be an indication of the susceptibility of the newborn infant to influenza infections.

A retrospective survey of transfusion hepatitis associated with a brand of commercial Factor VIII was carried out in 24 Haemophilia Centres from January 1974 until December 1975. Of 371 patients who were transfused with this product, and were followed up, 78 cases of hepatitis affecting 66 patients were found (17·7%). Two types of hepatitis were observed: hepatitis B and non-B hepatitis, the latter with an incubation period of between 8 and 60 days. Twelve patients contracted two types of hepatitis, non-B followed by hepatitis B. Only one patient died after contracting hepatitis B. Four of the suspect batches of concentrate were found to be positive for HBsAg by radioimmunoassay.

There was evidence that the presence of hepatitis B surface antibody in a patient's serum prior to exposure was associated with immunity to hepatitis B. Evidence was presented suggesting that the non-B hepatitis observed was not due to hepatitis A. The factors affecting the incidence of transfusion hepatitis in haemophiliacs were discussed.

Brains, spleens and livers of 2212 murids, 27 shrews and 7 dormice, trapped at 7 sites in Rhodesia, were tested in 277 pools for the presence of Rift Valley Fever virus. There were no isolations of Rift Valley Fever, but 69 isolations of an unidentified virus were obtained. Sixteen out of 867 sera had low-titre haemagglutination-inhibition activity against Rift Valley Fever antigen, but only one out of 1260 sera had neutralizing antibody. The evidence suggests that murids fail to encounter infection in nature and are unlikely to play a role in circulation and dissemination of Rift Valley Fever virus. Four out of seven widely distributed species of murid, Rhabdomys pumilio, Saccostomys campestris, Aethomys chrysophilus and Lemniscomys griselda, were shown to be capable of circulating amounts of virus likely to be infective for mosquitoes.

The effect of blood group status on the incidence of epidemic influenza A (H3N2) infections and on serological response to influenza vaccination with killed subunit and live attenuated vaccines have been investigated during comparative vaccine trials in Western Australia. A significantly higher incidence of epidemic influenza was observed in subjects of blood group B compared with those of other blood groups, regardless of whether they had serological evidence of previous exposure to H3N2 antigens or not. Volunteers of different blood groups exhibited similar seroconversion frequencies to both vaccines after the administration of two doses, but a significantly higher proportion of blood group A subjects seroconverted after receiving their first dose of live attenuated vaccine compared with those of other blood groups. Although this finding was inconsistent with the increased incidence of epidemic influenza in subjects of blood group B, it is discussed in terms of the methods employed to obtain attenuation. Higher geometric mean HI antibody titres were observed in blood group O subjects after the administration of killed subunit vaccine. The results described in this report supported the contention that genetic factors linked to ABO blood groups may play a role in susceptibility to infection with influenza A virus, but that any association must be indirect.

Titres of haemagglutination-inhibiting antibody have been measured repeatedly in young women during a period of 6–8 years after the administration of RA27/3 and Cendehill attenuated rubella vaccines. Mean antibody titres were initially 217 after RA27/3 and 159 after Cendehill, but the difference diminished after the first year. Antibody titres were subsequently well maintained in both groups and did not reveal any need for regular revaccination. Mean titres in the Cendehill group were partly maintained by symptomless reinfection which was commoner after Cendehill than after RA27/3. Significant falls in titre were equally common after both vaccines, but low titres of 30 or less were more frequent in subjects who had received Cendehill.

Mean neutralizing antibody titres were initially 15·4 after RA27/3 vaccine and 9 after Cendehill. Titres remained higher after RA27/3 for 3 years, but the difference then diminished and became insignificant during the fifth year.

Revaccination of women with low antibody titres produced significant increases in 69% of subjects when standard RA27/3 vaccine was used; a special preparation of RA27/3 of higher potency produced a similar number of rises (70%) but elicited higher titres and might occasionally be useful for revaccinating women who are likely to come into contact with rubella. Challenge with RA27/3 vaccine produced weaker responses in women who had experienced natural infection than in those whose antibody was vaccine-induced.

Rises in antibody titre after revaccination consisted mainly of IgG, but traces of IgM antibody were detected in one vaccinee who had recently experienced natural reinfection and in 1 woman with naturally acquired antibody who had been challenged with high titre RA27/3 vaccine.

Corynebacterium kutscheri and its alleged avirulent variant were re-examined in C57Bl/6 and Swiss Lynch mice. It was confirmed that while C57B1/6 mice were resistant and Swiss Lynch susceptible to C. kutscheri, the alleged atypical variant was avirulent in both mouse strains. However, following inimunosuppression of C57B1/6 mice with hydrocortisone acetate, it was not possible to reactivate latent C. kutscheri or the alleged atypical variant; this was contrary to previous reports. Moreover, sequential hysterectomy derivation over four generations of C57B1/6 mice did not eliminate their resistance to C. kutscheri compared with conventionally born animals. Vaccination with live attenuated C. kutscheri protected susceptible mice against virulent challenge; vaccination with the alleged atypical variant afforded no such protection. The suggested role of the alleged avirulent variant in resistance to C. kutscheri is challenged and an alternative explanation of such resistance is proposed.

Two-dimensional quantal microneutralization tests on foot-and-mouth disease viruses, in which neutralizing antibody activity was titrated against a serial range of virus doses, demonstrated a variety of dose-response curves some of which were rectilinear, others clearly curvilinear. Moreover, in the case of the non-linear responses obtained with some antisera, the shape of the curve was such that antibody titres recorded with doses of virus ranging from 103–105 TCD 50 were closely similar. Studies were carried out on the effect of varying the conditions of the test on the shape of the dose-response curve: significant differences were obtained after treatment of the antiserum–virus mixtures with anti-species globulin, and when the test was assayed in cells of differing susceptibility to infection.

Bluetongue antibody of 19 different serological types was found in a group of sentinel cattle near Nairobi. A continuous challenge by these 19 strains occurred each year in this location. The sero-conversion rates were shown to vary from year to year and rainfall had no obvious effect upon the conversion rates.

The 19 different strains of virus were also shown to be active at five widely scattered sites in different parts of the country.

The fluorescent antibody technique was used to investigate an epidemic of Klebsiella infection in a urological ward and to trace the probable source to a contaminated sink in the treatment room. It was also shown that cross infections by particular capsular types were very common within each ward. Certain types of Klebsiella occurred in cut-flower water but could not be associated with the types infecting the patients.

Antibiotic resistance patterns within one capsular type were found to vary whether the type was from different sources in one patient or from different patients in the same ward. One capsular type was observed to develop resistance to increasing numbers of antibiotics over a 3-year period. This was probably due to the acquisition of R-plasmids. There also appeared to be a relation between capsular type and the site of infection.

The frequency of Klebsiella infections in the urological wards dropped significantly after up-grading the treatment room, improving catheter storage and reducing ampicillin use.

A group of 20 990 children in Uganda was examined for leprosy over a period of 8 years. There was no evidence that the incidence of leprosy varied according to a child's genetic relationship to a leprosy patient, once allowance had been made for the grade of physical contact.

Morphological, biochemical and serological observations suggested that no close relationship existed between C. kutscheri and its alleged avirulent variant isolated from mice at the Rockefeller University. However, both capillary and double diffusion precipitin reactions showed the alleged variant to be a streptococcus of group N, indistinguishable from that previously isolated from Rockefeller University mice.

Groups of 33 chickens were fed continuously on diets containing feed additives that are employed commercially for a variety of purposes, and were infected orally when 4 days old with a nalidixic acid-resistant mutant of Salmonella typhimurium. The amount of S. typhimurium organisms excreted in their faeces was estimated by culturing them at intervals and in a standard manner on brilliant green agar containing sodium nalidixate; when the chickens were killed their caecal contents were examined by the same technique.

Avoparcin and lincomycin, like nitrovin and tylosin (Smith & Tucker, 1975b), favoured colonization of the alimentary tract by the S. typhimurium organisms as shown by the fact that the chickens to which they were fed excreted these organisms in their faeces in higher concentration and for longer periods of time than did chickens fed on non-medicated diets. Amprolium, monensin, dimetridazole, arsenilic acid and nitro-hydroxyphenylarsonate had no obvious effect on the salmonella excretion pattern.

When only five chickens in each group were experimentally infected so that the effect of the feed additives on infections acquired by contact could be monitored, avoparcin, lincomycin, nitrovin and tylosin again favoured colonization of the alimentary tract with the S. typhimurium organisms and so did dimetridazole. Arsenilic acid, in contrast, hindered the development of infection. Amprolium, monensin and nitro-hydroxyphenylarsonate were without obvious effect.

Many of the chickens that were fed on diets that favoured S. typhimurium colonization, but not those fed on non-medicated diets, were still excreting S. typhimurium organisms in their faeces when they were killed at 56 days of age, the age at which broiler chickens kept under commercial conditions are usually slaughtered.

In this investigation the bacterial contamination of surfaces such as walls and floors in a modern operating suite, together with surfaces of lamps in the operating theatres, and the clogs worn by staff, was studied. Counts of colony-forming units were made on impression plates containing blood-agar with Tween 80 for total bacterial counts, Baird Parker medium with egg yolk and tellurite for Staphylococcus aureus and trypticase peptone agar with neomycin and polymyxin for Clostridium spp.

The areas examined were divided into the patients' route to the operating theatre, the staff's route, and the central area containing the operating rooms, anaesthetic rooms, and exit and scrub-up areas. In the patients' route counts of total organisms ranged from about 10 000 to 30 000/m2 for Staph. aureus the range was from 70 to 540/m2. In the staff's route the highest count was about 70000/m2 in the dressing area, and the numbers of Staph. aureus were about the same as along the patients' route. In the inner zone the counts were somewhat lower for both total organisms and Staph. aureus. Total counts on the floor from all areas of the inner zone were significantly higher just before the second operation than before the first operation on the same day. The total and Staph. aureus counts on walls, floors and lamps were the same after clean operations as after operations classified as ‘contaminated’ or ‘dirty’.

A total of 1887 bovine sera positive to the Rose Bengal plate test were subjected to other serological tests for bovine brucellosis: the complement fixation test using warm fixation (CFTW), the serum agglutination test (SAT) and the radioimmunoassay (RIA).

The SAT was generally much less sensitive than the CFTW. Many sera, however, gave positive reactions in the SAT but no reaction in the CFTW or the RIA. These SAT reactions were attributed to IgM antibody.

Comparison between the results of the CFTW and the RIA led to the conclusion that 200 ng could be used as a minimum diagnostic reaction in the RIA.

Results obtained from 1887 sera using three serological tests for bovine brucellosis were compared with a serological classification of sera described as the ‘probable infection status’. Sera showing apparent false positive and apparent false negative reactions were identified, and were subjected to supplementary testing as appropriate.

The serum agglutination test (SAT) gave 35% apparent false negative reactions and 5% apparent false positives. The complement fixation test (CFT) gave 12% apparent false negative reactions using warm fixation (CFTW) and at least 5% using cold fixation (CFTC). The routine diagnostic system used in Victoria, in which the CFTW is supplemented by the CFTC and the SAT, gave 9% apparent false negative reactions and 2% apparent false positive reactions. The radioimmunoassay gave 1% or 6% apparent false negative reactions, depending the minimum diagnostic value used.

Atypical reactions in the CFT sometimes caused difficulties in diagnosis.

In a survey designed to determine the prevalence of Bacillus cereus in the faeces of healthy persons, the organism was found in low numbers in 100 (14%) of single faecal specimens from 711 adults in the general population. In addition, in an attempt at assessing the changes in the B. cereus distribution within the faecal flora of the individual, weekly faecal specimens were submitted over a seven-week period by 18 members of staff of two laboratories. The total isolation rate was again 14%, with 15 serotypes represented. In four individuals B. cereus was isolated in two consecutive weeks and in all cases the isolates were of different serotypes. Excretion was never recorded for more than two consecutive weeks. These findings probably reflect the intake of B. cereus in the individual's diet.