1. The myoglobin contents of selected muscles in horse, pig, domestic fowl and pigeon, and in the foetuses of horse, pig, sheep and cattle, have been determined.
2. The order of increasing concentration of myoglobin in the adult animal (4 years) is given by the series heart, longissimus dorsi, diaphragm and psoas, the figures for draught horse being, respectively, 0·325, 0·465, 0·610 and 0·705% and for pig, 0·203, 0·280, 0·350 and 0·435%, and the ratios of these concentrations being the same in each animal.
3. In both draught horse and pig, it is shown that the concentration of the pigment rises rapidly from birth, and that, after 2 years in the horse, and 1 year in the pig, it remains fairly constant, except in the case of horse psoas and diaphragm where there is a slow, but significant, rise throughout life.
4. This relationship of myoglobin with age has been shown to be significant at a probability level of 0·1%.