Information on density and abundance of globally threatened species such as tigers Panthera tigris is essential for effective conservation as well as to evaluate the success of conservation programmes. We monitored tigers in Parsa Widlife Reserve, Nepal, using camera traps, in 2013, 2014 and 2016. Once believed to be a sink for tigers from adjacent Chitwan National Park, Parsa now provides a new hope for tigers. Spatially explicit capture–recapture analysis over 3 survey years revealed an increase in tiger density from 0.78 to 1.38 individuals per 100 km2 from 2013 to 2016. The tiger abundance was estimated to be seven (6–13), 11 (10–16) and 17 (17–20) in 2013, 2014 and 2016, respectively. Resettlement of communities from the core area, reduced anthropogenic pressure, and improved security have made Parsa Wildlife Reserve a suitable habitat for tigers. Tiger abundance increased considerably within a 5 km radius of the evacuated village sites, from two in 2013 to eight in 2014 and 10 in 2016. Population turnover has remained moderate (< 30% per year), with persistence of individuals in multiple years. Dispersing tigers from Chitwan's source population accounted for a large portion (c. 40%) of the tigers detected in Parsa. Conservation efforts along with annual monitoring should be continued in Parsa to sustain the increase and monitor the persistence of tigers. The Chitwan–Parsa complex should be managed as a single ecological unit for conserving the Endangered tiger and other wide-ranging species.

Reliable information on wildlife populations and the threats they face is crucial for assessing the performance of conservation strategies. As part of its efforts to improve the effectiveness of Bukit Barisan Selatan National Park in Sumatra, Indonesia, and aid the recovery of flagship species, the Park's management authority designated a 1,000 km2 forest block an Intensive Protection Zone. To set a baseline from which to evaluate the performance of this zone, we investigated the density of tigers Panthera tigris sumatrae, and spatio-temporal interactions between tigers, their principle prey and threats. The estimated density of tigers was 2.8 per 100 km2, whereas in 2002 camera-trapping failed to record any tigers in the Intensive Protection Zone. We found the study area contained a rich prey base, with muntjac deer Muntiacus muntjak, macaques Macaca nemestrina and wild pigs Sus scrofa occupying 85–98% of the area, and sambar Rusa unicolor 61%. Despite these promising findings we also recorded a relatively high number of people entering the Park illegally, with 77 incidents over 6 months, of which 20% involved armed poachers. The poachers operated mainly at night and were concentrated in two locations. Law enforcement patrol teams were active during the day, and therefore had little overlap with the poachers. Prioritizing these at-risk areas for increased protection by rangers would further secure the Intensive Protection Zone, and expanding ranger activity across the Park would support efforts to remove the Park from UNESCO's List of World Heritage In Danger.

As part of a landscape-scale programme for conserving tigers Panthera tigris the Khata corridor was established between Bardia National Park in Nepal and Katarniaghat Wildlife Sanctuary in India in early 2000. We examined its functionality by comparing the status of tigers and prey in the corridor and in the adjacent National Park, using camera trapping, transect sampling and diet analysis of scats. Tiger movement was inferred from the photographs, and tiger–human conflict was assessed by means of questionnaires and interviews. The corridor harboured transient individuals as well as resident, breeding tigers. Tigers with core areas in the corridor were also recorded in the two protected areas, and vice versa. Wild prey was 3–4 times more abundant in the area of the National Park bordering the corridor than in the corridor itself, and domestic livestock constituted 12–15% of the tigers’ food in the corridor. Livestock losses and human fatalities or injuries were relatively low compared to within the buffer zones of the National Parks. Despite such problems and restrictions on grazing and extraction of natural resources, local residents were generally positive towards tigers and the corridor. The successful establishment of the corridor and the positive attitudes of local people were attributable to community development programmes initiated to compensate for the imposed restrictions, financed by the government and national and international organizations. By linking Bardia National Park and Katarniaghat Wildlife Sanctuary via the Khata corridor, a protected tiger landscape of c. 3,000 km2 was established in west-central Nepal and northern India.

We investigated the range of people involved in killing tigers Panthera tigris in the Bangladesh Sundarbans, their motives and methods, and their links to the commercial trade. Using snowball sampling we conducted 141 qualitative interviews with local people. We identified five categories (village residents, poachers, shikaris, trappers and pirates), each with different motives, methods and networks. Village residents kill tigers predominantly for safety, whereas others kill in the forest professionally or opportunistically. Poachers kill tigers for money, but for others the motives are more complex. The motives of local hunters are multifaceted, encompassing excitement, profit, and esteem and status arising from providing tiger parts for local medicine. Pirates kill tigers for profit and safety but also as a protection service to the community. The emerging international trade in tiger bones, introduced to the area by non-local Bangladeshi traders, provides opportunities to sell tiger parts in the commercial trade and is a motive for tiger killing across all groups.

Human–tiger conflict arises when tigers Panthera tigris attack people or their livestock, and poses a significant threat to both tigers and people. To gain a greater understanding of such conflict we examined spatio-temporal patterns, correlates, causes and contexts of conflict in Chitwan National Park, Nepal, and its buffer zone, during 2007–2014. Data, mostly from compensation applications, were collected from the Park office. Fifty-four human casualties (32 fatalities, 22 injuries) and 351 incidents of livestock depredation were recorded, clustered in defined areas, with 75.9% of human casualties occurring in the buffer zone and 66.7% within 1 km of the Park boundary. A linear model indicated there was a significant increase in human casualties during 2007–2014. Livestock were killed in proportion to their relative availability, with goats suffering the highest depredation (55%). There was a positive correlation between livestock depredation and National Park frontage (the length of Village Development Committee/municipality boundary abutting the National Park), but not human population, livestock population, forest area in the buffer zone, rainfall or temperature. There was no relationship between tiger attacks on people and any of the correlates examined. Wild prey density was not correlated with conflict. Of the tigers removed because of conflict, 73.3% were male. The majority of attacks on people occurred during accidental meetings (77.8%), mostly while people were collecting fodder or fuelwood (53.7%), and almost half (48.2%) occurred in the buffer zone forests. We recommend the use of the conflict map developed here in the prioritization of preventive measures, and that strategies to reduce conflict should include zoning enforcement, improvement of livestock husbandry, participatory tiger monitoring, an insurance scheme, and community awareness.

Difficult terrain and inclement weather limit our knowledge of large predators, such as the tiger Panthera tigris, in the Himalayas. A lack of empirical data on large carnivores can lead to mismanagement of protected areas and population declines. We used non-invasive genetic and remote sensing data to inform the management of such high-altitude protected areas. We used the tiger as a focal species to investigate prey preference and habitat suitability in India's Buxa Tiger Reserve, which encompasses several eco-geographical regions in the Himalayan and subtropical zones. During 2010–2013, 909 faecal samples were collected, of which 372 were confirmed, using genetic analysis, to be of tiger origin. Fourteen prey species/groups were identified in 240 tiger faecal samples, largely dominated by goats Capra spp. (26.59%), rhesus macaques Macaca mulatta (22.22%) and cattle Bos spp. (20.63%). Considering only the wild prey species for which survey data are available, however, and frequency of occurrence of prey in faecal samples, hog deer Axis porcinus, sambar deer Rusa unicolor and spotted deer Axis axis were the most preferred prey species. Using faecal sample locations to examine the relationship between tiger presence and environmental features indicated that the niche for tigers is narrower than the available protected area: c. 62% of core protected area is suitable, of which only 17% is highly suitable for tigers. Tigers prefer dense vegetation, open forests, riverine vegetation and areas close to water sources. Faecal sample-based studies have the potential to generate data that can help us understand the ecology of elusive carnivore species inhabiting high-altitude landscapes.

Meeting global and regional environmental targets is challenging, given the multiplicity of stakeholders and their diverse and often competing policy agendas and objectives. Relatively few studies have sought to systematically analyse the progress, or lack thereof, of institutionally complex and diffuse projects. Here we analyse one such project, which aims to protect and restore a critical landscape corridor for tigers Panthera tigris in north-western India, using a temporal–analytic framework that integrates ecological information on species population status and spatial connectivity modelling with a systematic examination of the decision-making process. We find that even with adequate ecological knowledge the tiger population is on the verge of local extinction because of weak institutional support, poor adaptive planning and ineffective leadership in a complex political arena, which has led to delays in conservation action. From the outset the conservation agencies and NGOs that were the primary drivers of the project lacked awareness of the political idiosyncrasies of coordinating the actions of disparate agencies within the decision-making process. To secure better future environmental outcomes we recommend the adoption of an improved project appraisal methodology that explicitly encompasses an evaluation of organizational incentives, to determine political buy-in, including alignment with organizational objectives and funding availability.

Many global efforts to decrease deforestation have focused on community-based conservation programmes to reach their goals. However, many such programmes are lacking a potentially helpful population, women. We employed key-informant interviews to examine attitudes towards, and barriers to, women's participation in a community-based conservation programme prior to implementation. We conducted 47 semi-structured interviews in January 2015 in communities adjacent to the Vaca Forest Reserve in the Cayo District of Belize. Results indicated that the benefits of involving women in community-based conservation activities included them learning more about the forest and conservation, transferring this knowledge to their family and community, and helping the environment. Some possible barriers to participation included lack of time and motivation to participate. However, there were notable differences between men's and women's responses relating to women's available time and their willingness to participate, with men not mentioning time as a barrier and stating that women would need more motivation to participate. The results will be used to work with local women to collaboratively develop and implement a community-based conservation programme around the Vaca Forest Reserve.

The bush dog Speothos venaticus is a medium-sized Neotropical canid. It is considered to be rare and its biology and population parameters are still poorly understood. The Amazon is one of the main strongholds of this species and is important for maintaining viable populations, as the region still holds extensive tracts of pristine habitat. We gathered field data from camera-trap studies throughout the Brazilian Amazon to estimate the relative abundance of the species and gain an understanding of its rarity, and how this compares with estimates from other vegetative formations and for sympatric hypercarnivores. We focused on three pristine or partially disturbed sites and one fragmented site. The estimated relative abundance of the species was 0.060–0.185 individuals per 100 trap-days, confirming that the species is rare. The bush dog's abundance in the Amazon is equivalent to that in all other areas outside the Basin. The mean group size recorded was c. 2.5 individuals. There were no differences in group sizes between forests in the Amazon and in other regions of Central America; however, there were significant differences between forests and open habitats. A combination of competition/predation, habitat structure/integrity, and disease may be acting synergistically in determining the abundance and rarity of bush dogs.

Over the last few decades botanic gardens worldwide have been encouraged to adopt complementary measures for the conservation of plant species from their own regions, combining in situ conservation efforts with ex situ methods, both in cultivation and in storage. This integrated approach is particularly important for botanic gardens in the tropics, which face the challenge of conserving a highly diverse and often threatened flora. We gathered information on the occurrence of threatened species in the natural vegetation reserves of 21 Brazilian botanic gardens. The data were collected from herbarium records in the database of the National Centre for Flora Conservation, and from the available plant inventories of these reserves. The results indicate that 148 species from the List of Threatened Species of Brazilian Flora are recorded as having been collected in the reserves. Of these, 51 species were maintained in the living collections of 18 botanic gardens and 83 species were recorded in federally protected areas. The occurrence of threatened species in the reserves of botanic gardens highlights the scientific value of these areas, as well as their biological, social and cultural importance for conservation. The results may be used to inform the planning of integrated conservation strategies for threatened species.

Habitat loss and climate change are major threats to amphibian species worldwide. We combined niche modelling under various climatic scenarios with analysis of habitat loss and the appropriateness of Cuban protected areas to identify major risk zones for the Endangered Cuban toad Peltophryne longinasus. Four subspecies with disjunct distributions associated with mountain forests are recognized. Our results suggest that the western subspecies, P. longinasus longinasus and P. longinasus cajalbanensis, are at risk from global warming, habitat degradation and potential additive effects. Peltophryne longinasus dunni, in central Cuba, has the lowest threat level related to climate change and habitat loss but could become increasingly threatened by the presence of the infectious disease chytridiomycosis. The eastern subspecies, P. longinasus ramsdeni, faces moderate impacts of climate change and habitat loss; however, low opportunity of migration to new areas and population decline justify a high threatened status for this subspecies. Our results predict minor temperature increases and precipitation decreases in the future. Nevertheless, at the biological level these changes could generate variations in species physiology, vocal behaviour and prey availability, and could probably increase the risk of predation. In Cuba protected areas have contributed to avoiding excessive forest loss but the potential impact of climate change was not considered in their original design. Our findings confirm that all subspecies of P. longinasus are threatened but management measures should be tailored according to the various predicted impacts.

The unprecedented political and economic reforms taking place in Myanmar offer new opportunities for biodiversity conservation. They also bring new challenges in the form of rapidly growing extractive industry and agriculture sectors that have been weakly regulated and are often unsustainable. The Endangered hog deer Axis porcinus epitomizes many of these conservation challenges, and those facing most deer species in the Indo–Burma hotspot. The hog deer has disappeared from large parts of its range as a result of overhunting and intense conversion of its floodplain grassland habitat for agriculture. We report on a population of hog deer that was discovered in the Indawgyi landscape in central Myanmar in 2012. We conducted the first rigorous assessment of a hog deer population in Myanmar using an occupancy sampling protocol, tested the protocol's robustness using a power analysis, and present the results to guide management intervention. The results from our study site revealed widespread occurrence of the species, with high precision. The population map was then used to inform the development of a conservation management zone within a UNESCO Man and Biosphere Reserve around Indawgyi Lake. The importance of this population for the status of the hog deer in Myanmar remains unknown because documentation of the species has been sparse. Our survey protocol could make a significant contribution to addressing this knowledge gap and setting an informed agenda for conservation of the hog deer both nationally and more widely across the Indo-Burma hotspot.

The Myanmar snub-nosed monkey Rhinopithecus strykeri was discovered in 2010 on the western slopes of the Gaoligong Mountains in the Irrawaddy River basin in Myanmar and subsequently in the same river basin in China, in 2011. Based on 2 years of surveying the remote and little disturbed forest of the Gaoligong Mountains National Nature Reserve in China, with outline transect sampling and infrared camera monitoring, a breeding group comprising > 70 individuals was found on the eastern slopes of the Gaoligong Mountains in the Salween River Basin. Given the Critically Endangered status of this primate (a total of < 950 individuals are estimated to remain in the wild), efforts to protect the relatively undisturbed habitat of this newly discovered population and to prevent hunting are essential for the long-term survival of this species.

The spoon-billed sandpiper Calidris pygmaea is a Critically Endangered shorebird that breeds in the Russian arctic and winters in coastal and estuarine habitats in South-east Asia. We report the first formal estimate of its global population size, combining a mark–resighting estimate of the number of leg-flagged individuals alive in autumn 2014 with an estimate of the proportion of birds with flags from scan surveys conducted during the same period at a migration stop-over site on the Jiangsu coast of China. We estimate that the world breeding population of spoon-billed sandpipers in 2014 was 210–228 pairs and the post-breeding population of all age classes combined was 661–718 individuals. This and related methods have considerable potential for surveillance of the population size of other globally threatened species, especially widely dispersed long-distance migrants.