This paper presents new census data and population estimates for penguins, blue-eyed shags, and southern giant petrels from 26 sites in the Antarctic Peninsula, collected by the Antarctic Site Inventory from 1994 to 2000. For nine sites, population data or estimates are published for the first time. The newly discovered gentoo penguin population of 215 nests at Herofna Island (63°24'S, 54°36'W) represents the easternmost location where this species has been found breeding in the Peninsula. All three pygoscelid penguins — gentoo, Adelie, and chinstrap — were found breeding at Gourdin Island (63° 12'S, 57° 18'W), the fourth known site where these species nest contiguously in the Peninsula. During the period, significant declines in nesting populations of blue-eyed shag were documented at three northwestern Peninsula locations.