A growth chamber study was conducted to evaluate the effects of temperature and soil moisture on the absorption and translocation of the new sulfonylurea herbicide, MON 37500, in wheat, jointed goatgrass, wild oat, and downy brome. Treatment combinations of temperatures—25/23, 15/13, and 5/3 C—and soil moistures of one-third, two-thirds, and full field capacities were assigned to the germinated plants. Radiolabeled MON 37500 was applied to the second leaf of plants at the three-leaf stage. The plants were then harvested at 6, 24, and 96 h after application. The plants absorbed MON 37500 in the greatest quantity during the first 6 h; however, absorption did continue to increase over time. Absorption was highest in wheat and lowest in wild oat. Temperature influenced absorption, while soil moisture status appeared to have no effect. Translocation of radiolabeled material out of the treated leaf increased over time; however, 90 to 97% of absorbed radioactivity remained in the treated leaf 96 h after treatment. Temperature affected translocation differently in each species, but increasing soil moisture increased translocation.

Soil-active herbicides were investigated for control of live oak (Quercus virginiana Mill.) on the Texas Coastal Prairie and Claypan areas with and without mechanical treatment. Roller chopping before, during, or after herbicide application had very little influence on mortality of live oak compared to the herbicide applied alone. Roller chopping did allow increased grazing of livestock for at least 3 yr after knockdown of the brush. Tebuthiuron {N-[5-(1,1-dimethylethyl)-1,3,4-thiadiazol-2-yl]-N,N′-dimethylurea} was generally the most effective herbicide for live oak control; bromacil (5-bromo-3-sec-butyl-6-methyluracil), karbutilate [tert-butylcarbamic acid ester with 3(m-hydroxyphenyl)-1,1-dimethylurea], and picloram (4-amino-3,5,6-trichloropicolinic acid) were somewhat less effective, especailly at 2.2 kg/ha. Limited data suggested that hexazinone [3-cyclohexyl-6-(dimethylamino)-1-methyl]-1,3,5-triazine-2,4(1H,3H)-dione] also was highly effective for control of live oak. Primary invaders on either mechanically or chemically treated areas were American beautyberry (Callicarpa americana L.), southern dewberry (Rubus trivialis Michx.), boneset (Eupatorium perfoliatum L.), yankeeweed (Eupatorium compositifolium Walt.), brownseed paspalum (Paspalum plicatulum Michx.), and carpetgrass (Axonopus affinis Chase). A herbicide was generally equally effective for live oak control whether applied broadcast or in rows as granules, or applied as sprays to the soil surface or subsurface.

Oxadiazon [2-tert-butyl-4-(2,4-dichloro-5-isopropoxyphenyl)-δ2-1,3,4-oxadiazolin-5-one] applied early enough for large crabgrass [Digitaria sanguinalis (L.) Scop.] control provided full season goosegrass [Eleusine indica (L.) Gaertn.] control during bermudagrass [Cynodon dactylon (L.) Pers. ‘Midiron’ and ‘Tufcoat’] establishment from sod strips. Prosulfalin {N-[[4-(dipropylamino)-3,5-dinitrophenyl]sulfonyl]-S,S-dimethylsulfilimine}, DCPA (dimethyl tetrachloroterephthalate), pendimethalin [N-(1-ethylpropyl)-3,4-dimethyl-2,6-dinitrobenzenamine] and bensulide [0,0-diisopropyl phosphorodithioate S-ester with N-(2-mercaptoethyl)benzenesulfonamide] gave full season goosegrass control in well-established Midiron bermudagrass, but not during the initial establishment period where open bare soil was present. Napropamide [2-(α-naphthoxy)-N,N-diethylpropionamide] provided long term residual control of goosegrass with some interference with rooting of bermudagrass from stolon nodes. Winter survival of Midiron bermudagrass was superior to Tufcoat bermudagrass. Controlling annual grasses permitted superior bermudagrass growth, and winter survival was improved tremendously. Postemergence goosegrass control was obtained with MSMA (monosodium methanearsonate) and methazole [2-(3,4-dichlorophenyl)-4-methyl-1,2,4-oxadiazolidine-3,5-dione] when repeated several times during the season. A mixture of MSMA and methazole was superior to either used alone. The rate of bermudagrass establishment was increased through the control of goosegrass with herbicides.

Glyphosate [N-(phosphonomethyl)glycine] consistently controls many winter annual weeds in dormant bermudagrass [Cynodon dactylon (L.) Pers.] but injures actively growing turf. Experiments were conducted to determine the effects of dates of glyphosate treatments on control of winter annuals in different stages of maturity and on the tolerance of bermudagrass when applied to the turf at different stages of greenness from dormant turf in the winter to actively growing turf in the spring. Glyphosate was applied at rates of 0, 0.3, 0.6, 1.1, and 2.2 kg/ha to different plots of winter annuals and bermudagrass at 3-week intervals from February 13 to May 3. Glyphosate at 0.3 kg/ha controlled at least 90% of annual bluegrass (Poa annua L.), spur weed (Soliva sessilis P. & K.), and corn speedwell (Veronica arvensis L.) within a 6-week period regardless of the date of treatment. All glyphosate treatments injured bermudagrass when applied to semi-dormant or actively growing turf. Bermudagrass treated with glyphosate at 0.3 kg/ha was injured the least and the turf had essentially recovered by 6 weeks after treatment. However, the injury from higher rates (0.6 to 2.2 kg/ha) was too severe to be acceptable in turf areas. Glyphosate did not severely injure bermudagrass when applied to dormant turf.

Waterhyacinth [Eichornia crassipes (Mart.) Solms.] was partially controlled with 10.6 nm laser radiation. Radiation levels of < 1 J/cm2 for individually irradiated plants and 69 J/cm2 for group-irradiated plants significantly inhibited growth. Photosynthesis in treated plant-leaf tissue was inhibited about 50% by an exposure level of 4 J/cm2. 14C-labeled compounds fixed by plant-leaf tissue following laser treatment were found to be normally distributed.

Nineteen different genera of weeds encompassing twelve plant families were surveyed for hydrolytic enzymes which were able to metabolize the amide bonds in several groups of herbicides. Propanil (3′,4′-dichloropropionanilide), 1,1-dimethyl-3-phenylurea (fenuron), and isopropyl carbanilate (propham) were employed as enzyme substrates, representing the amide, urea, and carbamate classes. Propanil was hydrolyzed at widely diverse rates by approximately 70% of the plants tested. Only one plant showed a hydrolysis of propham and fenuron. The study demonstrates a widespread distribution of hydrolytic enzymes capable of degrading the herbicide, propanil. An apparent lack of hydrolytic enzymes able to metabolize propham and fenuron was also noted.

The effect of 67 g ai/ha chlorsulfuron {2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)-amino] carbonyl] benzenesulfonamide} on Canada thistle [Cirsium arvense (L.) Scop. ♯ CIRAR] root bud growth was examined in a series of greenhouse trials in which potted plants were treated with foliar sprays. Injury to root buds was assayed by determining their ability to form secondary shoots. Added surfactant, 0.2% (v/v) oxysorbic [oxysorbic (20 POE) polyethylene sorbitan monooleate], did not enhance chlorsulfuron-induced inhibition of parent shoot growth, but it increased root bud injury from foliarly applied chlorsulfuron. Cuttings taken from controls formed more secondary shoots than did chlorsulfuron-treated plants 2 weeks following spraying. However, root fresh weight and final secondary shoot growth from cut roots were unchanged 3 weeks after chlorsulfuron treatment compared to the time of spraying. Foliar treatment or a combination of foliar and soil treatment inhibited root fresh weight accumulation and secondary shoot growth equally 1 month following treatment relative to harvest controls. Soil treatment alone did not reduce either root fresh weight gains or secondary shoot outgrowth from root buds. Foliar treatment of vegetative Canada thistle with chlorsulfuron inhibited subsequent secondary shoot outgrowth from root buds more than did treatment at flowering.

Barnyardgrass [Echinochloa crus-galli (L.) Beauv. # ECHCG] spikelets were collected from greenhouse-grown plants at various developmental stages. Caryopses contained in a single spikelet were dormant at maturity, but dormancy was overcome when the spikelets were dry afterripened at 23 C or were treated with high temperatures, acid scarification, or punctured with a scalpel. The season at which anthesis occurred affected the length of time for afterripening. Enhanced germination due to puncturing and scarification was not related to removal of a restriction to water entry. The effectiveness of puncturing in overcoming dormancy depended on moisture content of the spikelets at the time of puncturing. Above 18% moisture content germination percentage was low regardless of the dormancy-releasing treatment.

Pitted morningglory is an adaptable species with an indigenous range encompassing the southern Midwest and southeast United States. In 2000 through 2002, 64 pitted morningglory accessions from 11 states were grown in Fayetteville, AR, to compare their morphology in a common environment to document potential morphological variation and to determine whether variation proves the existence of pitted morningglory morphological ecotypes. Accessions were evaluated for leaf size and vine length 8 wk after emergence (WAE), date of flower initiation, flower color, leaf pubescence 12 WAE, capsule and sepal pubescence, sepal length and width, plant weight, and seed number at physiological maturity. Morphological variables were standardized and analyzed with cluster analysis to differentiate the morphological variation among accessions. Documented variation was best described by eight clusters. Four clusters distinguished themselves morphologically. Accessions within these distinct clusters were originally from Arkansas, Delaware, Kentucky, Louisiana, Mississippi, and Missouri and were documented with leaf size, vine length, and day of flower initiation generally increasing with decreasing latitude. The other four clusters were nondistinct because most variables differed very little, but characteristics such as capsule pubescence separated these clusters. Accessions within these nondistinct clusters originated from Arkansas, Georgia, Louisiana, North Carolina, Oklahoma, and Tennessee. Leaf shapes of arrow, heart, an arrow and heart mixture, and heart with pointed projections and white or purple flower colors were documented. Documented differences indicate the existence of pitted morningglory morphological ecotypes.

The translocation of disodium methanearsonate (DSMA) and amine methanearsonate (hereinafter referred to as AMA) in purple nutsedge (Cyperus rotundus L.) was studied following foliar applications. Tracer techniques with DSMA-14C and chemical analyses for elemental arsenic (hereinafter referred to as As) suggested apoplastic and symplastic movement of both arsenicals. Chromatographic studies of extracts from DSMA-14C-treated plants indicated that the compound was not readily broken down. The carbon-arsenic bond appeared to remain largely intact, although some 14C activity was found in respiratory CO2 several days after treatment. A comparison between the Rf value of the radioactive plant extract to that of standard DSMA-14C suggested that a plant extract-DSMA complex may have been formed. Over 85% of the material applied to the plant remained in the treated shoots. DSMA moved in both acropetal and basipetal directions, and such movement within a single leaf was not influenced by relative leaf age. The arsenicals were not redistributed in the shoot or in the tuber system, but As was accumulated in terminal tubers following repeated applications.

The mode of action of quinclorac was investigated in broadleaf and grass species. Quinclorac induced characteristic auxinlike symptoms in broadleaf species but not in susceptible grasses. In susceptible grasses, quinclorac caused necrotic bands near the zones of elongation in shoots and roots. Electrolyte leakage was induced by quinclorac in smooth crabgrass and other susceptible grasses but not in tolerant grass or susceptible broadleaf species. In smooth crabgrass, increased electrolyte leakage and reduced fresh weight were rate dependent, and initially specific to young tissues. An inhibitory effect on elongation in the youngest leaf of smooth crabgrass and in primary roots of corn was detected 6 and 3 h after quinclorac treatment, respectively. Electrolyte leakage required more than 12 and 6 h in the leaf and root, respectively. Depolarization of corn root cell membrane potential was not observed in a 6-h treatment period. Results presented here provide additional evidence that quinclorac activity differs between susceptible broadleaf and grass species. In addition, the action of quinclorac appears to be similar in both shoot and root tissues of susceptible grasses. It is proposed that quinclorac-induced electrolyte leakage in susceptible grasses is a secondary response and that the primary mechanism of action involves inhibition of an as yet unknown metabolic process associated with cell expansion.

Field studies were conducted near Tifton, GA, from 1995 to 1997 to measure Cyperus esculentus (yellow nutsedge) interference with ‘Fancipack’ Cucumis sativus (cucumber) using a response prediction experiment with a natural infestation of C. esculentus. Cucumis sativus was direct-seeded each year. Plots (1.8 by 1.8 m) were established immediately after crop emergence. Cyperus esculentus plants were counted 2 wk after crop emergence in each plot, at which time four weed-free plots were randomly established. C. esculentus densities ranged from 0 to 955 plants m−2. Total yield, plant biomass, and C. sativus stand were regressed against C. esculentus density and biomass. Regression analysis showed a 5% reduction in C. sativus yield with a C esculentus infestation of approximately 15 plants m−2. A uniform C. sativus stand maximized its competitive ability and minimized interference from C. esculentus. Cyperus esculentus was more competitive and reduced C. sativus yields when C. sativus stands were low and nonuniform.

Eight herbicide postemergence treatments were applied to proso millet (Panicum milaceum L.) at three growth stages. The dimethylamine salt of (2,4-dichlorophenoxy)acetic acid (2,4-D) at 0.56 kg/ha had significantly higher grain yields than the weedy check. All herbicides except the amine of 2,4-D at 0.28 kg/ha appeared to injure proso millet plants by varying degrees; however, yields were not greatly affected. All herbicides gave excellent control of redroot pigweed (Amaranthus retroflexus L.) when applied to proso millet in the 4 to 6-leaf stage. Weed control was poorer when spraying was delayed until proso millet was in pre-boot and post-flower stages.

Dalapon (2,2-dichloropropionic acid), tebuthiuron {N-[5-(1,1-dimethylethyl)-1,3,4-thiadiazol-2-yl]-N,N′-dimethylurea}, and hexazinone [3-cyclohexyl-6-(dimethylamino)-1-methyl-1,3,5-triazine-2,4(1H,3H)-dione] were evaluated for smutgrass [Sporobolus poiretii (R.&S.) Hitchc] control in bahiagrass (Paspalum notatum Flugge.) pastures. Dalapon and hexazinone provided the best smutgrass control but injured the bahiagrass. Forage treated with hexazinone at 1.7 kg/ha or less recovered within 6 months; however, the effects of the initial injury from dalapon at 2.2 kg/ha could be detected for as long as 2 yr after treatment. Dogfennel [Eupatorium capillifolium (Lam.) Small] and broomsedge (Andropogon virginicus L.) replaced smutgrass and bahiagrass removed by dalapon. Higher rates of tebuthiuron than hexazinone (3.4 vs. 1.1 kg/ha) were required to control smutgrass adequately.

Several soil-applied and two foliar herbicides were evaluated for weed control in established alfalfa (Medicago sativa L.) in two experiments for two consecutive years. Most of the soil-applied herbicides effectively controlled weeds in the first harvest year following application; however, there was little carry-over into succeeding years. Except for the low rate of secbumeton [N-ethyl-6-methoxy-N′ (1-methylpropyl)-1,3,5-triazine-2,4-diamine] in the first alfalfa cutting of 1973, no herbicide treatment significantly increased alfalfa yields over the control plot. In fact, some herbicides caused injury to alfalfa at high rates of application, as did reapplication a second year at the original rate of application.

Simulated sprayer tank residues of the broadleaf weed herbicides dicamba, chlorsulfuron, and clopyralid applied alone and with the grass weed herbicides sethoxydim and diclofop on sunflower, tame mustard, and lentil, respectively, caused visible crop injury and reduced dry weight and yield. Dry weight production in the greenhouse and crop tolerance ratings in the field indicated that the grass weed herbicides enhanced crop injury from dicamba, chlorsulfuron, and clopyralid. Yield reductions in field experiments were also greater when dicamba and clopyralid were mixed with grass weed herbicides and applied on sunflower and lentil, respectively. This did not occur with chlorsulfuron applied to mustard. When mixed with simulated broadleaf weed herbicide residues, diclofop enhanced dry weight reductions and crop injury and reduced yield to a greater extent than sethoxydim. Crop tolerance ratings differentiated treatments and rates but were not a good estimate of the extent of yield loss. When broadleaf weed herbicides were applied at rates simulating sprayer tank residues alone or combined with grass weed herbicides, yield losses ranged up to 40% in sunflower, 70% in mustard, and 95% in lentil, compared to the untreated check.

Recently, several incidents of glyphosate failure on junglerice [Echinochloa colona (L.) Link] have been reported in the midsouthern United States, specifically in Mississippi and Tennessee. Research was conducted to measure the magnitude of glyphosate resistance and to determine the mechanism(s) of resistance to glyphosate in E. colona populations from Mississippi and Tennessee. ED50 (dose required to reduce plant growth by 50%) values for a resistant MSGR4 biotype, a resistant TNGR population, and a known susceptible MSGS population were 0.8, 1.62, and 0.23 kg ae ha−1 of glyphosate, respectively. The resistance index calculated from the these ED50 values indicated that the MSGR4 biotype and TNGR population were 4- and 7-fold, respectively, resistant to glyphosate relative to the MSGS population. The absorption patterns of [14C]glyphosate in the TNGR and MSGS populations were similar. However, the MSGS population translocated 13% more [14C]glyphosate out of the treated leaf compared with the TNGR population at 48 h after treatment. EPSPS gene sequence analyses of TNGR E. colona indicated no evidence of any point mutations, but several resistant biotypes, including MSGR4, possessed a single-nucleotide substitution of T for C at codon 106 position, resulting in a proline-to-serine substitution (CCA to TCA). Results from quantitative polymerase chain reaction analyses suggested that there was no amplification of the EPSPS gene in the resistant populations and biotypes. Thus, the mechanism of resistance in the MSGR population (and associated biotypes) is, in part, due to a target-site mutation at the 106 loci of the EPSPS gene, while reduced translocation of glyphosate was found to confer glyphosate resistance in the TNGR population.

The spring-tine harrow is gaining popularity for mechanical weeding. However, its weeding performance and mechanism have not been well understood. A spring-tine harrow was first tested in a controlled indoor soil bin at four different travel speeds (4, 6, 8, and 10 km h−1) with three different spring-loading settings (low, medium, and high). Then the harrow was tested in a wheat (Triticum aestivum L.) field at the same spring-loading settings at three different weeding timings (early, middle, and late) in 2019 and 2020. Soil cutting forces (draft and vertical), soil displacements (forward and lateral), soil working depth, weed control efficacy, weed density, and crop damage were measured. The results showed that the spring-loading setting had a more dominant effect on working depth and soil cutting forces than the speed. The soil displacements were more dependent on the speed compared with the spring-loading setting. Treatment effects on weeding performance indicators in the field were similar across years. Adjusting the spring-loading setting from low to high improved the weeding efficacy from 44.9% to 73.9% in 2019 and from 51.6% to 78.1% in 2020. Consequently, the final weed density was minimized at the high loading setting, with the reduction in 2020 being significant. The middle weeding timing caused the least crop damage, while reducing the final weed density by approximately one-third compared with the control (without mechanical weeding), which was the most desired outcome among the three timings tested.

Seeds of witchweed (Striga asiatica) were found in undisturbed Lakeland sand in all layers to 60 inches deep.

Waterhemp [Amaranthus tuberculatus (Moq.) Sauer] is one of the most troublesome agronomic weeds in the midwestern United States. The rapid evolution and selection of herbicide-resistance traits in A. tuberculatus is a major challenge in managing this species. An A. tuberculatus population, designated CHR, was identified in 2012 in Champaign County, IL, and previously characterized as resistant to herbicides from six site-of-action groups: 2,4-D (Group 4), acetolactate synthase inhibitors (Group 2), protoporphyrinogen oxidase inhibitors (Group 14), 4-hydroxyphenylpyruvate dioxygenase inhibitors (Group 27), photosystem II inhibitors (Group 5), and very-long-chain fatty-acid synthesis inhibitors (Group 15). Recently, ineffective control of CHR was observed in the field after dicamba application. Therefore, this research was initiated to confirm dicamba resistance, quantify the resistance level, and investigate its inheritance in CHR. Multiple field trials were conducted at the CHR location to confirm poor control with dicamba and compare dicamba treatments with other herbicides. Greenhouse trials were conducted to quantify the resistance level in CHR and confirm genetic inheritance of the resistance. In field trials, dicamba did not provide more than 65% control, while glyphosate and glufosinate provided at least 90% control. Multiple accessions were generated from controlled crosses and evaluated in greenhouse trials. Greenhouse dicamba dose–response experiments indicated a resistance level of 5- to 10-fold relative to a sensitive parental line. Dose–response experiments using F1 lines indicated that dicamba resistance was an incompletely dominant trait. Segregation analysis with F2 and backcross populations indicated that dicamba resistance had moderate heritability and was potentially a multigenic trait. Although dicamba resistance was predominantly inherited as a nuclear trait, minor maternal inheritance was not completely ruled out. To our knowledge, CHR is one of the first cases of dicamba resistance in A. tuberculatus. Further studies will focus on elucidating the genes involved in dicamba resistance.