The objective of this project is to integrate a domestic photocatalytic desulphurization facility with a biogas upgrading module and try to develop a system for biogas desulphurization and upgrading under ambient conditions. Four photocatalytic desulphurization reactors (PDRs) and one activated carbon reactor (ACR) were applied for biogas desulphurization and filtration under ambient conditions. Moreover, a hollow fibre carbon dioxide (CO2) adsorption module was applied for biogas upgrading. The operation pressure of the PDR and ACR was under ambient pressure. Results showed that hydrogen sulphide removal efficiency of the photocatalytic desulphurizer was about 0.99–1.00 (v/v) under the inlet biogas flow less than 5 litres/min and the concentration of inlet hydrogen sulphide was lower than 5600 mg/m3. For desulphurized biogas upgrading, the removal efficiency of CO2 was higher than 0.90 (v/v) under the outlet biogas flow was 1 litre/min (i.e. inlet biogas flow was about 2 litres/min). However, the ratio of methane in the upgrading biogas was lower than 0.90 (v/v). Thus, nitrogen gas removal cartridges will be integrated with the biogas upgrading module to promote methane concentration in the upgraded biogas.

High temperature negatively affects cotton production worldwide. In Pakistan, cotton crop faces high temperature at peak flowering during June–July, which is a major reason for yield losses. The present study was conducted to find some agro-physiological markers for high-temperature tolerance. Fifty cotton genotypes were raised under normal sown (high-temperature stress) and late sown (optimum temperature) conditions for 2 years. Data were recorded for relative cell injury percentage (RCI), chlorophyll content (CC), canopy temperature (CT), boll retention percentage (BR), bolls per plant (BP), boll weight (BW), hundred seed weight (HSW), sympodial branches per plant (SBP), plant height (PH) and seed cotton yield (SCY). A large amount of variability was found among cotton genotypes as proved by descriptive statistics. Stable genotypes were selected based on higher SCY and yield components (BP, BW, HSW, SBP, PH and SCY) performance along with less RCI%, high CC and low CT. The results of heatmap analysis showed that the six cotton genotypes viz., FH-Noor, FH-Lalazar, FH-458, FH-466, NIAB-545 and NIAB-878 performed better under high-temperature stress. These selected genotypes can be a source for breeding high-temperature tolerance. Furthermore, the results from correlation analysis confirmed that the traits such as RCI%, CC and CT can be considered as early selection criteria due to their positive association with SCY. Whilst some other parameters such as BP, BW, BR, SBP and PH might be used as direct selection indices for SCY under high-temperature stress.

Agricultural waste contributes significantly to greenhouse gas (GHG) emissions if not adequately recycled and sustainably managed. A recurring agricultural waste is livestock waste that has consistently served as feedstock for biogas systems. The objective of this study was to assess the use of animal waste digestate to mitigate GHG emissions in agricultural fields. Wheat (Triticum spp. L.) was fertilized with different types of animal waste digestate (organic fertilizers) and synthetic nitrogen fertilizer (inorganic fertilizer). The 170 kg N/ha presented in digestates were split fertilized at an application rate of 90 and 80 kg N/ha. Emissions of GHGs (carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O)) were monitored directly by a static chamber system. The soil and environmental variables were measured to determine their influence on GHG emissions. Emission peaks in N2O and CO2 after the first application of fertilizers with the emissions flattening out over the cultivating season while CH4 emission was negligible with no apparent patterns observed. Results showed individual and cumulative emissions of CO2, CH4 and N2O from the digestates were relatively low and digestate fertilization could be an efficient method for reducing GHGs from agricultural sources in temperate climate conditions.

A field experiment was carried out for over two seasons (autumn and spring) as a split–split plot scheme based on a randomized complete block design with three replications. The main plots included two irrigation levels of the maximum available water depletion (maximum allowable depletion (MAD)) of 55 and 85% as non-stress and drought-stress environments, respectively, and the subplot accommodated two levels of nitrogen (0 and 62.5 kg N/ha, urea fertilizer); also, 20 barley genotypes were assigned to the sub-subplots. The biplot analysis of both sowing seasons showed that grain yield (GY) had a high positive correlation with total biomass (TB), whereas it had a high negative correlation with proline and total soluble carbohydrate as drought-tolerance-determinant characteristics. The genotypes which had the lowest and highest GY ranked significantly (P ≤ 0.01) different with changing the sowing season under each irrigation level, indicating a larger plant interaction and non-stability in response to the season change (about two-fold), as compared to the change in the irrigation conditions. It could also be concluded that barley genotypes might experience a higher decrease in GY and sensitivity to water deficit in the autumn sowing season, as compared to the spring planting season, which was also intensified by nitrogen application. However, the response to nitrogen application depends on the plant genotype.

The production of specialty coffee has several factors and parameters that are added up in the course of production, so that the quality is expressed in the act of consumption. Based on this scenario, this study included the analysis of ten genotypes of arabica coffee, the materials being subjected to irrigated and rainfed water regimes, in a low altitude region, to identify responses for sensory and physical–chemical quality. The genotypes were evaluated in a split-plot scheme with a randomized block design, with three replications. Arabica coffee fruits were harvested with 80% cherry seeds and processed by the wet method. Subsequently, the characteristics related to physical–chemical and sensory analyses were evaluated. The genotypes of the Paraíso group showed great variability for the physical–chemical and sensory variables for rainfed and irrigated regimes. The genotypes of the Catuaí group, however, showed less variability for sensory characteristics in both cultivation environments and for physical–chemical characteristics in the irrigated regime. In the sensorial data set, the genotypes Catuaí 144 CCF and Catuaí 144 SFC (when irrigated) and Paraíso H 419-3-3-7-16-2, Paraíso H 419-3-3-7-16-11 and Catucaí 24-137 (rainfed cultivation), are more favourable to the production of specialty coffee at low altitude.

Grain-filling, as the final growth stage of rice, is sensitive to environmental temperature change. Previous studies mainly concerned about the effects of high temperature stress during grain-filling on rice growth, and most experiments were carried out with pot for cultivating rice and greenhouse for warming. This research investigated the response of rice grain-filling of superior spikelets (SS) and inferior spikelets (IS) of two japonica cultivars to elevated temperature during grain-filling stage under open-field warming conditions in lower reaches of Yangtze River Basin using free-air temperature enhancement facility. Results indicated that rice yield was not significantly changed by warming less than 4°C. SS and IS showed different responses to elevated temperature during the grain-filling stage, whereas there were similar trends between two cultivars and years. For SS, although elevated temperature enhanced its filling rate during the early grain-filling period, and caused a shorter grain-filling period and a lighter grain weight; for IS, elevated temperature improved its grain weight by enhancing its filling rate during middle and late grain-filling period due to the increased number of days with suitable temperature. For both SS and IS, key starch biosynthesis enzymes and indole-3-acetic acid content exhibited generally a similar dynamics trend with grain-filling rates, and these sink strength parameters presented higher levels under elevated temperature relative to natural temperature for IS during middle and late grain-filling period. Consequently, warming less than 4°C presented different influences on SS and IS; the improvement of IS filling under warming regime was associated with the intensification of grain sink strength.

The main aim of the current study was to present the abilities of widely used crop models to simulate four different field crops (winter wheat, spring barley, silage maize and winter oilseed rape). The 13 models were tested under Central European conditions represented by three locations in the Czech Republic, selected using temperature and precipitation gradients for the target crops in this region. Based on observed crop phenology and yield from 1991 to 2010, performances of individual models and their ensemble were analyzed. Modelling of anthesis and maturity was generally best simulated by the ensemble median (EnsMED) compared to the ensemble mean and individual models. The yield was better simulated by the best models than estimated by an ensemble. Higher accuracy was achieved for spring crops, with the best results for silage maize, while the lowest accuracy was for winter oilseed rape according to the index of agreement (IA). Based on EnsMED, the root mean square errors (RMSEs) for yield was 1365 kg/ha for winter wheat, 1105 kg/ha for spring barley, 1861 kg/ha for silage maize and 969 kg/ha for winter oilseed rape. The AQUACROP and EPIC models performed best in terms of spread around the line of best fit (RMSE, IA). In some cases, the individual models failed. For crop rotation simulations, only models with reasonable accuracy (i.e. without failures) across all included crops within the target environment should be selected. Application crop models ensemble is one way to increase the accuracy of predictions, but lower variability of ensemble outputs was confirmed.

Factorial experiments were carried out on spring- and winter-sown oat crops in Ireland in 2016–2019 to identify plant responses in yield formation and grain filling procedures. Two cultivars (Husky and Keely), three seed rates (250, 350 and 450 seeds/m2), six applied nitrogen (N) rates (40, 70, 100, 130, 160 and 190 kg N/ha) with a plant growth regulator (PGR) were examined in spring and winter sowings, using the same cultivars at five rates of applied N (80, 110, 140, 170 and 200 kg N/ha). The applied N rate and seed rate significantly (P < 0.05) modified the yield components determining grains/m2 in spring- and winter-sown crops with increases in panicle number of key importance. Increases were also observed in the proportion of primary grain by weight (0.9–1.6%) and number (0.7–1.4%) ratios in spring-sown crops in response to applied N rate, with winter-sown crops exhibiting a more consistent pattern of grain fill. Seed rate and PGR application had minor effects on yield components and panicle conformation. Grain fill procedures played an important role in the maximization of grain yield under seasonal conditions. Significant positive correlations were observed between the number of aborted grain sites and yield under drought conditions (0.22), with negative associations observed in near-optimal conditions (−0.22 to −0.41). Agronomic effects on grain site abortion were minimal in comparison with seasonal effects. In seasons characterized by cool, consistent conditions, grain yield was maximized by the utilization of all available grain sites. Where assimilate shortages were encountered during grain fill the abortion of grains sites was positively associated with grain yield.

The North China Plain suffers excessive application of nitrogen (N) and soil degradation. Recently, biochar has been promoted as an agricultural soil amendment to ameliorate soil quality, increase crop yield and mitigate greenhouse gas emissions. However, most proofs on the positive effects of biochar addition have been based on small plots or short-term field studies located in tropical or subtropical regions with defective soils. A long-term field experiment was designed with five N levels and two biochar rates to observe the changes in crop (winter wheat and summer maize) growth and soil chemical quality. Notably, crop yield was strongly dependent upon N application, with both wheat and maize yields increasing with N application. Biochar addition increased crop yield but not significantly, although the increase in grain yield was 0.96 t/ha in a rotation. Correlation analysis revealed that the optimal root-layer soil mineral N (Nmin) for crop production was around N120, especially with biochar addition. The effect of fertilization on soil chemical quality was mainly reflected in the increase in soil organic carbon (SOC), and the highest value was obtained at N60 whether or not biochar was applied. Overall, biochar addition did not appear to promote wheat and maize growth or increase the yield on calcareous alluvial soils in temperate regions, but significantly enhanced SOC content, especially at N60, which may play an important role in sustainable agricultural production.

This study evaluated the yield and water productiivty response of quinoa to regulated deficit irrigation (RDI), partial root-zone drying (PRD) and conventional deficit irrigation (DI) and full irrigation (FI) using surface (SD) and subsurface drip (SSD) systems in 2016 and 2017 in the eastern Mediterranean region of Turkey. The treatments consisted of RDI, PRD50, DI50, DI75 and FI. A rainfed treatment (RF) was also included in the study. The experimental design was split plots with four replications. DI75 and DI50 received 75 and 50% of FI, respectively. PRD50 received 50% of FI, but from alternative laterals. RDI received 50% of FI during vegetative stage until flowering, and then received 100% of water requirement. The results showed that quinoa under SD used slightly more water than SSD due to reduced surface evaporation. RDI resulted in water saving of 23 and 21% for SD and SSD, respectively, compared to FI; and RDI produced statistically similar grain yields to FI. DI75 treatment resulted in water savings of 16% for both drip methods in the first year and 10 and 25% for SD and SSD systems, respectively, in the second year. PRD50 produced greater yield than DI50 eventhough they received the same amount of irrigation water. RF and PRD50 treatments resulted in significantly greater water productivity (WP) values than other treatments. There was no significant difference between SD and SSD regarding the grain and dry matter yields and WP values. Thus, RDI and DI75 appear to be good alternatives to FI for sustainable quinoa production in the Mediterranean region.

Glutamine synthetase (GS) and glutamate synthase (GOGAT) play a central role in plant nitrogen (N) metabolism. In order to study the effect of powdery mildew (Blumeria graminis f. sp. tritici, Bgt) on N metabolism, field experiments were carried out to evaluate GS and GOGAT activity, GS expression and grain protein content (GPC) in susceptible (Xi'nong 979) and resistant (Zhengmai 103) wheat cultivars under three treatments. The three treatments were no inoculation (CK), inoculated once with Bgt (MP) and inoculated nine times with Bgt (HP). For Xi'nong 979, the activities of GS and GOGAT in grains as well as GS activity in flag leaves increased at 10–15 days after anthesis (DAA), and decreased significantly at 15 or 20–30 DAA in HP and MP. However, GS activity in grains decreased from 20 DAA, which was later than that of flag leaves (15 DAA). At the same time, GS expression in grains was up-regulated at early stage, with GS1 at 10 DAA and GS2 at 15 DAA, followed by a continuous down-regulation. This result indicated that GS and GOGAT activity as well as GS expression were inhibited by powdery mildew, indicating that N metabolism in grains was inhibited at 20–30 DAA. The current study also found out that the yield of the susceptible cultivar decreased significantly, while its GPC increased obviously in HP. It was shown that the increase of GPC was not due to the enhancement of N metabolism, but due to the passive increase caused by yield reduction.

This work aimed to evaluate the effects of sire's and dam's biological type, dam's age class at calving and individual heterozygosis, and to estimate variance components for weaning weight adjusted to 210 days (WW210) in beef cattle of different breed groups. Records of 13 687 animals, obtained from 2000 to 2007, were used. Bulls from the biological types Zebu (N), Adapted (A), British (B), Continental (C) and ¼N|¼A|¼B|¼C were mated with purebred zebu (N) and crossbred females (½C|½N and ½B|½N). Dam age at calving was 3–12 years. The influence of several effects on WW210 was tested using the least square method. Variance component analysis was performed using a Bayesian approach. The model included contemporary group, dam's age class at calving, sire's and dam's biological types as systematic effects, animal's age and individual heterozygosis as linear covariates, and direct and maternal additive genetic, maternal permanent environmental and residual effects as random effects. The progeny of bulls from biological type B and the crossbred cows showed higher WW210 means. Cows at 6–7 years old weaned heavier calves. Direct and maternal heritability estimates for WW210 were 0.5 ± 0.04 and 0.1 ± 0.02, respectively. Calves with 100% individual heterozygosis weighed on average 25.98 kg more at weaning compared to progenies from pure breeds. Sire's and dam's biological types influence the WW210 of the crossed progenies. Crossbred cows produce heavier calves compared to biological type N cows. These results and the obtained direct and maternal heritabilities suggest it is possible to choose the lines of sires and dams that could be used to make the crosses to obtain progenies with better performance for WW210.

The present study was conducted to evaluate the effect of a live culture of Aspergillus oryzae (A; CCT4359) and fibrolytic enzyme (E; Fibrozyme Alltech Inc.) on fibre digestibility by a gas production bioassay and in vitro degradation of maize silage and sugarcane silage. A completely randomized design trial was performed to evaluate: A doses (0, 20, 60 and 100 mg/l), E doses (0, 160, 320 and 480 mg/l) and roughage source (R; maize and sugarcane silage) in a 4 × 4 × 2 factorial arrangement. The inclusion of increasing doses of A and E increased dry matter and neutral detergent fibre in vitro digestibility linearly, but for E this effect occurred only in maize silage. There was a linear increase in the potential for gas production at the highest dose of A only in sugarcane silage, with no effect on lag time (L). Increasing doses of E increased the volume of gases produced linearly, and a trend of linear reduction of L, regardless of the roughage. There was a linear reduction in ammonia-nitrogen concentration in response to increasing doses of A and E, and an increase in acetic acid concentration at the highest dose of A, regardless of roughage. The additives had no synergistic effect on gas production and digestibility, but were efficient in altering the fermentative pattern, demonstrating the potential to increase fibre degradation.